LOCUS LT726884 6765 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-HA-mABIN-1(54-647), complete
sequence.
ACCESSION LT726884
VERSION LT726884.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-HA-mABIN-1(54-647)
ORGANISM Mammalian expression vector pCAGGS-HA-mABIN-1(54-647)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6765)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6765
/organism="Mammalian expression vector
pCAGGS-HA-mABIN-1(54-647)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5388."
/db_xref="taxon:1944869"
misc_feature 15..41
/note="HA epitope"
CDS 66..1850
/transl_table=11
/note="mABIN-1(54-647)"
/protein_id="SJL86767.1"
/translation="MEASRLRQKAEELVKDSELSPPTSAPSLVSFDDLAELTGQDTKV
QVHPATSTAATTTATATTGNSMEKPEPASKSPSNGASSDFEVVPTEEQNSPETGSHPT
NMMDLGPPPPEDSNLKLHLQRLETTLSVCAEEPDHSQLFTHLGRMALEFNRLASKVHK
NEQRTSILQTLCEQLRQENEALKAKLDKGLEQRDLAAERLREENTELKKLLMNSSCKE
GLCGQPSSPKPEGAGKKGVAGQQQASVMASKVPEAGAFGAAEKKVKLLEQQRMELLEV
NKQWDQHFRSMKQQYEQKITELRQKLVDLQKQVTELEAEREQKQRDFDRKLLLAKSKI
EMEETDKEQLTAEAKELRQKVRYLQDQLSPLTRQREYQEKEIQRLNKALEEALSIQAS
PSSPPAAFGSPEGVGGHLRKQELVTQNELLKQQVKIFEEDFQRERSDRERMNEEKEEL
KKQVEKLQAQVTLTNAQLKTLKEEEKAKEALKQQKRKAKASGERYHMEPHPEHVCGAY
PYAYPPMPAMVPHHAYKDWSQIRYPPPPVPMEHPPPHPNSRLFHLPEYTWRPPCAGIR
NQSSQVMDPPPDRPAEPESADNDCDGPQ"
regulatory 2055..2146
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 2147..2503
/note="3' FR of beta-globin"
regulatory complement(2581..2663)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2805..2880
/note="SV40 ori"
regulatory 2911..2985
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2911..2985)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3100..3501)
/note="pMB1 ori"
CDS complement(4042..4902)
/transl_table=11
/note="ampicillin"
/protein_id="SJL86768.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5038..5415
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5421..6762
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5790..6714
/note="intron AG promoter"
BASE COUNT 1528 a 1845 c 1897 g 1495 t
ORIGIN
1 ttgaattcat ggcttaccca tacgatgttc cagattacgc tagcttgggt ggtcatatgg
61 ccgcgatgga agcgtccaga ctccggcaga aggcagagga gctggtcaag gacagcgagc
121 tgtcaccacc gacatctgcc ccctccttgg tctcctttga tgacctggct gagctcacag
181 gacaggatac aaaggtccag gtacatcctg ctaccagcac tgccgccacc accaccgcca
241 ccgccaccac gggaaactcc atggagaagc ccgagccagc ctccaaatct ccgtccaatg
301 gcgcctcctc ggactttgaa gtggtcccta ctgaggagca gaattcaccc gaaactggca
361 gccaccctac gaacatgatg gacctggggc ccccaccccc agaggacagc aacctgaagc
421 tccacctgca gcgcctggag accaccctta gcgtgtgtgc agaggagcca gaccacagcc
481 agctcttcac ccacctgggc cgcatggccc tcgagttcaa caggttggcc tccaaagtgc
541 ataaaaatga gcagcgcacc tccatcctgc agaccttatg tgagcagctg cgccaggaga
601 atgaagccct gaaggccaag ctggacaagg gcctggaaca gcgggatctg gctgctgaga
661 ggctgcggga ggaaaacacg gagctcaaga aactgttgat gaacagcagc tgcaaagagg
721 gactctgtgg gcagcccagc tccccaaagc cagagggtgc tggcaagaag ggcgtggctg
781 gacagcagca ggccagtgtg atggcgagta aagtccctga agcgggggcc tttggagcag
841 ctgagaagaa agtgaagttg ctagaacagc aacgcatgga gctgctggaa gtgaacaagc
901 agtgggacca gcatttccgg tccatgaagc agcagtatga gcagaagatc acagagcttc
961 gccagaagct ggtggacctg cagaaacagg taactgagct ggaggccgaa cgggagcaga
1021 agcagcgtga ctttgaccgg aaactcctcc tggccaaatc gaagatagag atggaagaga
1081 ccgacaagga gcagctgaca gcagaggcca aggaactgcg ccagaaggtc aggtacctac
1141 aggatcagct gagcccgctc acaaggcaac gagaatacca ggagaaggag atccagcggc
1201 tcaataaggc cctggaggag gccctcagca tccaggcctc tccatcatct ccgcctgcag
1261 cttttgggag tccagaaggc gttgggggcc atctgaggaa gcaggaacta gtgacacaga
1321 atgagttgct gaaacagcag gtaaagatct ttgaagagga cttccagagg gaacggagtg
1381 accgtgaacg catgaatgaa gagaaggagg agctgaagaa gcaagtagag aagctgcagg
1441 cccaggtcac cctgactaat gcccagctca aaactctcaa agaggaggag aaggccaagg
1501 aagccctcaa acagcagaag aggaaagcaa aggcttcggg agagcgctac cacatggaac
1561 cccaccctga gcacgtctgc ggcgcctatc cctatgccta cccacccatg ccagccatgg
1621 tacctcacca tgcctacaag gactggtccc agatccgata ccctccaccc cctgtgccca
1681 tggagcaccc gcccccacac cccaactctc gcctcttcca tctgccggag tacacctggc
1741 gtccaccctg tgcagggatt cggaatcaga gctctcaagt gatggacccg cccccagaca
1801 ggcctgcaga gccagagtct gcagacaatg actgtgatgg gccccagtga ggctgcagtg
1861 ggtcatttgg ttccaccttc atctttcaga gccagctgac ctcagattgc caaaagtttg
1921 aaggccatgt gcatgttctg tgtgacccaa gccttggcag aggagaggct gggatgggta
1981 gctggctcac atccccagcc aagcctcgaa ctgttgacaa gacccccaat gccctggctc
2041 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
2101 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
2161 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
2221 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
2281 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
2341 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
2401 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
2461 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
2521 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
2581 ccacacaaca tacgagccgg aagcataaag tgtaaagcct gggtgcctaa tgagtgagct
2641 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
2701 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
2761 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
2821 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
2881 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
2941 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
3001 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
3061 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
3121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
3181 taatacggtt atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca
3241 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
3301 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
3361 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
3421 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
3481 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3541 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
3601 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
3661 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
3721 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
3781 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
3841 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
3901 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
3961 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
4021 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
4081 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
4141 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
4201 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
4261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
4321 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
4381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
4441 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
4501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
4561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
4621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
4681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
4741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
4801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
4861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
4921 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
4981 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac
5041 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
5101 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
5161 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
5221 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
5281 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
5341 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
5401 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
5461 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
5521 gggcgggggg gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg
5581 gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
5641 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
5701 tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
5761 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
5821 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
5881 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
5941 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
6001 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
6061 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
6121 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
6181 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
6241 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
6301 cgcctcgggc cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc
6361 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
6421 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
6481 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
6541 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
6601 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
6661 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
6721 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaa
//