LOCUS LT726877 6006 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pEntry-L4 rtTA-IRES-Puro-pA-Ins/Ins-TRE R1, complete
sequence.
ACCESSION LT726877
VERSION LT726877.1
KEYWORDS .
SOURCE Vector pEntry-L4 rtTA-IRES-Puro-pA-Ins/Ins-TRE R1
ORGANISM Vector pEntry-L4 rtTA-IRES-Puro-pA-Ins/Ins-TRE R1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6006)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6006
/organism="Vector pEntry-L4 rtTA-IRES-Puro-pA-Ins/Ins-TRE
R1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 8212."
/db_xref="taxon:1945296"
misc_feature 15..257
/note="chicken beta-globin 5'HS4 insulator core sequence"
misc_feature 271..513
/note="chicken beta-globin 5'HS4 insulator core sequence"
regulatory 532..900
/note="hCMVT promoter"
/regulatory_class="promoter"
regulatory complement(1030..1153)
/note="attR1"
/regulatory_class="attenuator"
CDS 1277..2086
/transl_table=11
/note="Tn903 neomycin resistance"
/protein_id="SJL86749.1"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 2546..2948
/note="pMB1 ori"
regulatory complement(3027..3070)
/note="T1 terminator"
/regulatory_class="terminator"
regulatory 3202..3229
/note="T2 terminator"
/regulatory_class="terminator"
regulatory 3303..3398
/note="attL4"
/regulatory_class="attenuator"
CDS <3496..4500
/codon_start=1
/transl_table=11
/note="rtTA"
/protein_id="SJL86750.1"
/translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLKGESWQDFLRNNAKSFRCALLSHRNGAKVHSDT
RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETP
TTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYG
STIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVA
MAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG
"
misc_feature 4501..5044
/note="5' UTR of the EMCV polyprotein gene (IRES)"
CDS 5045..5644
/transl_table=11
/note="puromycin resistance"
/protein_id="SJL86751.1"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
regulatory 5731..5955
/note="BGH polyA"
/regulatory_class="terminator"
BASE COUNT 1455 a 1581 c 1622 g 1348 t
ORIGIN
1 ggccgcggcg cgccgagctc acggggacag ccccccccca aagcccccag ggatgtaatt
61 acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg
121 ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc
181 acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg
241 atacggggaa aaagcttggc caaaaaggcc gagctcacgg ggacagcccc cccccaaagc
301 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
361 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
421 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
481 tgcagacacc tgggggatac ggggaaaaag cttttaatta agcggccgca ctcgagttta
541 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
601 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
661 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
721 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
781 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc
841 gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg
901 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
961 atccagcctc cgcggccccg aattcggtac cggatccagt cgagatatct agaaagggcg
1021 aattcgaccc aagtttgtac aaaaaagttg aacgagaaac gtaaaatgat ataaatatca
1081 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
1141 tgcagtcact atgatgcagt cactatggct ctggcccgtg tctcaaaatc tctgatgtta
1201 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
1261 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
1321 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
1381 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
1441 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
1501 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
1561 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
1621 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
1681 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
1741 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
1801 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
1861 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
1921 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
1981 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
2041 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
2101 ttggttgtaa cattattcag attgggcccc gttccactga gcgtcagacc ccgtagaaaa
2161 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
2221 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
2281 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
2341 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
2401 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
2461 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
2521 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc
2581 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
2641 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
2701 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
2761 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
2821 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
2881 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
2941 ggaagagcgc ccaatacgca aaccgctagc atggatctcg gggacgtcta actactaagc
3001 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcggaa gactgggcct
3061 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
3121 cggatttgaa cgttgtgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa
3181 ctgccaggca tcaaactaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
3241 aaactcttcc tgttagttag ttacttaagc tcgggcccgc gttaacgcta ccatggagct
3301 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca
3361 atgctttttt ataatgccaa ctttgtatag aaaagttggc tccgaattcg cccttttaaa
3421 ggaaccaatt cagtcgaggt cgacggtatc gataagcttg atatcgaatt caccatgcca
3481 aagagaccca gaccctctag attagataaa agtaaagtga ttaacagcgc attagagctg
3541 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gcttggtgta
3601 gagcagccta cactgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc
3661 attgagatgt tagataggca ccatactcac ttttgccctt taaaagggga aagctggcaa
3721 gattttttac gcaataacgc taaaagtttt agatgtgctt tactaagtca tcgcaatgga
3781 gcaaaagtac attcagatac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa
3841 ttagcctttt tatgccaaca aggtttttca ctagagaacg cgttatatgc actcagcgct
3901 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa
3961 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta
4021 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga
4081 ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca gccgcgcgcg tacgaaaaac
4141 aattacgggt ctaccatcga gggcctgctc gatctcccgg acgacgacgc ccccgaagag
4201 gcggggctgg cggctccgcg cctgtccttt ctccccgcgg gacacacgcg cagactgtcg
4261 acggcccccc cgaccgatgt cagcctgggg gacgagctcc acttagacgg cgaggacgtg
4321 gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg ggacggggat
4381 tccccgggtc cgggatttac cccccacgac tccgccccct acggcgctct ggatatggcc
4441 gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta cggtgggtag
4501 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
4561 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
4621 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
4681 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
4741 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
4801 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
4861 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
4921 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
4981 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
5041 taatatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
5101 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
5161 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
5221 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
5281 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
5341 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
5401 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
5461 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
5521 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
5581 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
5641 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
5701 gatgatctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
5761 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
5821 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
5881 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
5941 cggtgggctc tatggcttct gaggcggaaa gaacctgcag cccgggggat ccactagttc
6001 tagagc
//