LOCUS LT726876 5707 bp DNA circular SYN 06-FEB-2017 DEFINITION Vector pENTR1A-NLS-LacZ, complete sequence. ACCESSION LT726876 VERSION LT726876.1 KEYWORDS . SOURCE Vector pENTR1A-NLS-LacZ ORGANISM Vector pENTR1A-NLS-LacZ other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5707) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5707 /organism="Vector pENTR1A-NLS-LacZ" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 8208." /db_xref="taxon:1945294" regulatory 1..96 /note="attL2" /regulatory_class="attenuator" CDS complement(375..>3422) /codon_start=1 /transl_table=11 /note="lacZ" /protein_id="SJL86746.1" /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVN PPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLP SEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGG YADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLL LLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYT LCDRYGLYVVDEANIETHGMVPMNLLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSL GNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQS LIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLS GQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPES AGQLWLTVRVVQPNATGWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFC IELGNKRWQFNRQSGFLSQLWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAW VERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQM AITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPL SDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEE GTWLNIDGFHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK" CDS complement(<3441..>3461) /codon_start=1 /transl_table=11 /note="SV40 large T NLS" /protein_id="SJL86747.1" /translation="PKKKRKV" regulatory complement(3581..3676) /note="attL1" /regulatory_class="attenuator" CDS 3800..4609 /transl_table=11 /note="Tn903 neomycin resistance" /protein_id="SJL86748.1" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 5069..5394 /note="pMB1 ori" regulatory complement(5455..5498) /note="T1 terminator" /regulatory_class="terminator" regulatory 5630..5657 /note="T2 terminator" /regulatory_class="terminator" BASE COUNT 1363 a 1472 c 1484 g 1388 t ORIGIN 1 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat 61 gctttcttat aatgccaact ttgtacaaga aagctgggtc tagagtcgag gccgagtttg 121 tcagaaagca gaccaaacag cggttggaat aatagcgaga acagagaaat agcggcaaaa 181 ataatacccg tatcactttt gctgatatgg ttgatgtcat gtagccaaat cgggaaaaac 241 gggaagtagg ctcccatgat aaaaaagtaa aagaaaaaga ataaaccgaa catccaaaag 301 tttgtgtttt ttaaatagta cataatggat ttccttacgc gaaatacggg cagacatggc 361 ctgcccggtt attattattt ttgacaccag accaactggt aatggtagcg accggcgctc 421 agctgtaatt ccgccgatac tgacgggctc caggagtcgt cgccaccaat ccccatatgg 481 aaaccgtcga tattcagcca tgtgccttct tccgcgtgca gcagatggcg atggctggtt 541 tccatcagtt gctgttgact gtagcggctg atgttgaact ggaagtcgcc gcgccactgg 601 tgtgggccat aattcaattc gcgcgtcccg cagcgcagac cgttttcgct cgggaagacg 661 tacggggtat acatgtctga caatggcaga tcccagcggt caaaacaggc ggcagtaagg 721 cggtcgggat agttttcttg cggccctaat ccgagccagt ttacccgctc tgctacctgc 781 gccagctggc agttcaggcc aatccgcgcc ggatgcggtg tatcgctcgc cacttcaaca 841 tcaacggtaa tcgccatttg accactacca tcaatccggt aggttttccg gctgataaat 901 aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa tcagcaccgc atcagcaagt 961 gtatctgccg tgcactgcaa caacgctgct tcggcctggt aatggcccgc cgccttccag 1021 cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac ttacgccaat gtcgttatcc 1081 agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca gttgtttttt atcgccaatc 1141 cacagctgtg aaagaaagcc tgactggcgg ttaaattgcc aacgcttatt acccagctcg 1201 atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg cgtgggacgc ggcggggagc 1261 gtcacactga ggttttccgc cagacgccac tgctgccagg cgctgatgtg cccggcttct 1321 gaccatccgg tcgcgttcgg ttgcactacg cgtactgtga gccagagttg cccggcgctc 1381 tccggctgcg gtagttcagg cagttcaatc aactgtttac cttgtggagc gacatccaga 1441 ggcacttcac cgcttgccag cggcttacca tccagcgcca ccatccagtg caggagctcg 1501 ttatcgctat gacggaacag gtattcgctg gtcacttcga tggtttgccc ggataaacgg 1561 aactggaaaa actgctgctg gtgttttgct tccgtcagcg ctggatgcgg cgtgcggtcg 1621 gcaaagacca gaccgttcat acagaactgg cgatcgttcg gcgtatcgcc aaaatcaccg 1681 ccgtaagccg accacgggtt gccgttttca tcatatttaa tcagcgactg atccacccag 1741 tcccagacga agccgccctg taaacgggga tactgacgaa acgcctgcca gtatttagcg 1801 aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc aaaggatcag cgggcgcgtc 1861 tctccaggta gcgaaagcca ttttttgatg gaccatttcg gcacagccgg gaagggctgg 1921 tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg tggccgtggt gtcggctccg 1981 ccgccttcat actgcaccgg gcgggaagga tcgacagatt tgatccagcg atacagcgcg 2041 tcgtgattag cgccgtggcc tgattcattc cccagcgacc agatgatcac actcgggtga 2101 ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca tcgccggtag ccagcgcgga 2161 tcatcggtca gcagattcat tggcaccatg ccgtgggttt caatattggc ttcatccacc 2221 acatacaggc cgtagcggtc gcacagcgtg taccacagcg gatggttcgg ataatgcgaa 2281 cagcgcacgg cgttaaagtt gttctgcttc atcagcagga tatcctgcac catcgtctgc 2341 tcatccatga cctgaccatg cagaggatga tgctcgtgac ggttaacgcc tcgaatcagc 2401 aacggcttgc cgttcagcag cagcagacca ttttcaatcc gcacctcgcg gaaaccgaca 2461 tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac caccgcacga 2521 tagagattcg ggatttcggc gctccacagt ttcgggtttt cgacgttcag acgtagtgtg 2581 acgcgatcgg cataaccacc acgctcatcg ataatttcac cgccgaaagg cgcggtgccg 2641 ctggcgacct gcgtttcacc ctgccataaa gaaactgtta cccgtaggta gtcacgcaac 2701 tcgccgcaca tctgaacttc agcctccagt acagcgcggc tgaaatcatc attaaagcga 2761 gtggcaacat ggaaatcgct gatttgtgta gtcggtttat gcagcaacga gacgtcacgg 2821 aaaatgccgc tcatccgcca catatcctga tcttccagat aactgccgtc actccaacgc 2881 agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag gtcaaattca 2941 gacggcaaac gactgtcctg gccgtaaccg acccagcgcc cgttgcacca cagatgaaac 3001 gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca gctttcatca 3061 acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa cggcggattg 3121 accgtaatgg gataggttac gttggtgtag atgggcgcat cgtaaccgtg catctgccag 3181 tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca gctttccggc 3241 accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct gcgcaactgt 3301 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 3361 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 3421 acgggatccc ccatgctccc cactttgcgt ttcttcttgg gccccatggt ggatcctgag 3481 actggggaat tccccggatc cgtcgatcga ctctagatat ctcgagtgcg gccgcgaatt 3541 cggtaccgga tccagtcgac tgaattggtt cctttaaagc ctgctttttt gtacaaagtt 3601 ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa 3661 aataaaatca ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg 3721 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa 3781 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa 3841 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc 3901 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca 3961 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac 4021 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt 4081 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc 4141 aggtgaaaat attgttgatg cgctggcagt gtccctgcgc cggttgcatt cgattcctgt 4201 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat 4261 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga 4321 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca 4381 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga 4441 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct 4501 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc 4561 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt 4621 taattggttg taacattatt cagattgggc cccgttccac tgagcgtcag accccgtaga 4681 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 4741 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 4801 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 4861 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 4921 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 4981 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 5041 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 5101 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 5161 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 5221 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 5281 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 5341 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgctagcat 5401 ggatctcggg gacgtctaac tactaagcga gagtagggaa ctgccaggca tcaaataaaa 5461 cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 5521 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga 5581 gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc 5641 ctgacggatg gcctttttgc gtttctacaa actcttcctg ttagttagtt acttaagctc 5701 gggcccc //