LOCUS       LT726876                5707 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Vector pENTR1A-NLS-LacZ, complete sequence.
ACCESSION   LT726876
VERSION     LT726876.1
KEYWORDS    .
SOURCE      Vector pENTR1A-NLS-LacZ
  ORGANISM  Vector pENTR1A-NLS-LacZ
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Vector pENTR1A-NLS-LacZ"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 8208."
                     /db_xref="taxon:1945294"
     regulatory      1..96
                     /note="attL2"
                     /regulatory_class="attenuator"
     CDS             complement(375..>3422)
                     /codon_start=1
                     /transl_table=11
                     /note="lacZ"
                     /protein_id="SJL86746.1"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
                     SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVN
                     PPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLP
                     SEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
                     VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGG
                     YADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLL
                     LLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYT
                     LCDRYGLYVVDEANIETHGMVPMNLLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSL
                     GNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQS
                     LIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLS
                     GQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPES
                     AGQLWLTVRVVQPNATGWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFC
                     IELGNKRWQFNRQSGFLSQLWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAW
                     VERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQM
                     AITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPL
                     SDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEE
                     GTWLNIDGFHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             complement(<3441..>3461)
                     /codon_start=1
                     /transl_table=11
                     /note="SV40 large T NLS"
                     /protein_id="SJL86747.1"
                     /translation="PKKKRKV"
     regulatory      complement(3581..3676)
                     /note="attL1"
                     /regulatory_class="attenuator"
     CDS             3800..4609
                     /transl_table=11
                     /note="Tn903 neomycin resistance"
                     /protein_id="SJL86748.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      5069..5394
                     /note="pMB1 ori"
     regulatory      complement(5455..5498)
                     /note="T1 terminator"
                     /regulatory_class="terminator"
     regulatory      5630..5657
                     /note="T2 terminator"
                     /regulatory_class="terminator"
BASE COUNT         1363 a         1472 c         1484 g         1388 t
ORIGIN      
        1 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat
       61 gctttcttat aatgccaact ttgtacaaga aagctgggtc tagagtcgag gccgagtttg
      121 tcagaaagca gaccaaacag cggttggaat aatagcgaga acagagaaat agcggcaaaa
      181 ataatacccg tatcactttt gctgatatgg ttgatgtcat gtagccaaat cgggaaaaac
      241 gggaagtagg ctcccatgat aaaaaagtaa aagaaaaaga ataaaccgaa catccaaaag
      301 tttgtgtttt ttaaatagta cataatggat ttccttacgc gaaatacggg cagacatggc
      361 ctgcccggtt attattattt ttgacaccag accaactggt aatggtagcg accggcgctc
      421 agctgtaatt ccgccgatac tgacgggctc caggagtcgt cgccaccaat ccccatatgg
      481 aaaccgtcga tattcagcca tgtgccttct tccgcgtgca gcagatggcg atggctggtt
      541 tccatcagtt gctgttgact gtagcggctg atgttgaact ggaagtcgcc gcgccactgg
      601 tgtgggccat aattcaattc gcgcgtcccg cagcgcagac cgttttcgct cgggaagacg
      661 tacggggtat acatgtctga caatggcaga tcccagcggt caaaacaggc ggcagtaagg
      721 cggtcgggat agttttcttg cggccctaat ccgagccagt ttacccgctc tgctacctgc
      781 gccagctggc agttcaggcc aatccgcgcc ggatgcggtg tatcgctcgc cacttcaaca
      841 tcaacggtaa tcgccatttg accactacca tcaatccggt aggttttccg gctgataaat
      901 aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa tcagcaccgc atcagcaagt
      961 gtatctgccg tgcactgcaa caacgctgct tcggcctggt aatggcccgc cgccttccag
     1021 cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac ttacgccaat gtcgttatcc
     1081 agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca gttgtttttt atcgccaatc
     1141 cacagctgtg aaagaaagcc tgactggcgg ttaaattgcc aacgcttatt acccagctcg
     1201 atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg cgtgggacgc ggcggggagc
     1261 gtcacactga ggttttccgc cagacgccac tgctgccagg cgctgatgtg cccggcttct
     1321 gaccatccgg tcgcgttcgg ttgcactacg cgtactgtga gccagagttg cccggcgctc
     1381 tccggctgcg gtagttcagg cagttcaatc aactgtttac cttgtggagc gacatccaga
     1441 ggcacttcac cgcttgccag cggcttacca tccagcgcca ccatccagtg caggagctcg
     1501 ttatcgctat gacggaacag gtattcgctg gtcacttcga tggtttgccc ggataaacgg
     1561 aactggaaaa actgctgctg gtgttttgct tccgtcagcg ctggatgcgg cgtgcggtcg
     1621 gcaaagacca gaccgttcat acagaactgg cgatcgttcg gcgtatcgcc aaaatcaccg
     1681 ccgtaagccg accacgggtt gccgttttca tcatatttaa tcagcgactg atccacccag
     1741 tcccagacga agccgccctg taaacgggga tactgacgaa acgcctgcca gtatttagcg
     1801 aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc aaaggatcag cgggcgcgtc
     1861 tctccaggta gcgaaagcca ttttttgatg gaccatttcg gcacagccgg gaagggctgg
     1921 tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg tggccgtggt gtcggctccg
     1981 ccgccttcat actgcaccgg gcgggaagga tcgacagatt tgatccagcg atacagcgcg
     2041 tcgtgattag cgccgtggcc tgattcattc cccagcgacc agatgatcac actcgggtga
     2101 ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca tcgccggtag ccagcgcgga
     2161 tcatcggtca gcagattcat tggcaccatg ccgtgggttt caatattggc ttcatccacc
     2221 acatacaggc cgtagcggtc gcacagcgtg taccacagcg gatggttcgg ataatgcgaa
     2281 cagcgcacgg cgttaaagtt gttctgcttc atcagcagga tatcctgcac catcgtctgc
     2341 tcatccatga cctgaccatg cagaggatga tgctcgtgac ggttaacgcc tcgaatcagc
     2401 aacggcttgc cgttcagcag cagcagacca ttttcaatcc gcacctcgcg gaaaccgaca
     2461 tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac caccgcacga
     2521 tagagattcg ggatttcggc gctccacagt ttcgggtttt cgacgttcag acgtagtgtg
     2581 acgcgatcgg cataaccacc acgctcatcg ataatttcac cgccgaaagg cgcggtgccg
     2641 ctggcgacct gcgtttcacc ctgccataaa gaaactgtta cccgtaggta gtcacgcaac
     2701 tcgccgcaca tctgaacttc agcctccagt acagcgcggc tgaaatcatc attaaagcga
     2761 gtggcaacat ggaaatcgct gatttgtgta gtcggtttat gcagcaacga gacgtcacgg
     2821 aaaatgccgc tcatccgcca catatcctga tcttccagat aactgccgtc actccaacgc
     2881 agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag gtcaaattca
     2941 gacggcaaac gactgtcctg gccgtaaccg acccagcgcc cgttgcacca cagatgaaac
     3001 gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca gctttcatca
     3061 acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa cggcggattg
     3121 accgtaatgg gataggttac gttggtgtag atgggcgcat cgtaaccgtg catctgccag
     3181 tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca gctttccggc
     3241 accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct gcgcaactgt
     3301 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3361 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3421 acgggatccc ccatgctccc cactttgcgt ttcttcttgg gccccatggt ggatcctgag
     3481 actggggaat tccccggatc cgtcgatcga ctctagatat ctcgagtgcg gccgcgaatt
     3541 cggtaccgga tccagtcgac tgaattggtt cctttaaagc ctgctttttt gtacaaagtt
     3601 ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     3661 aataaaatca ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg
     3721 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     3781 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     3841 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     3901 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     3961 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     4021 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     4081 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     4141 aggtgaaaat attgttgatg cgctggcagt gtccctgcgc cggttgcatt cgattcctgt
     4201 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     4261 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     4321 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     4381 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     4441 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     4501 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     4561 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     4621 taattggttg taacattatt cagattgggc cccgttccac tgagcgtcag accccgtaga
     4681 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4741 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4801 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     4861 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4921 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4981 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5041 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5101 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5161 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5221 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5281 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5341 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgctagcat
     5401 ggatctcggg gacgtctaac tactaagcga gagtagggaa ctgccaggca tcaaataaaa
     5461 cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     5521 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga
     5581 gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc
     5641 ctgacggatg gcctttttgc gtttctacaa actcttcctg ttagttagtt acttaagctc
     5701 gggcccc
//