LOCUS LT726875 6273 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pEntry-attR2-IRES-eGFP-luc+-pA-attL3, complete sequence.
ACCESSION LT726875
VERSION LT726875.1
KEYWORDS .
SOURCE Vector pEntry-attR2-IRES-eGFP-luc+-pA-attL3
ORGANISM Vector pEntry-attR2-IRES-eGFP-luc+-pA-attL3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6273)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6273
/organism="Vector pEntry-attR2-IRES-eGFP-luc+-pA-attL3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 8200."
/db_xref="taxon:1945295"
regulatory complement(14..109)
/note="attL3"
/regulatory_class="attenuator"
regulatory complement(149..165)
/note="T7 promoter"
/regulatory_class="promoter"
CDS 299..1108
/transl_table=11
/note="Tn903 neomycin resistance"
/protein_id="SJL86743.1"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 1625..2027
/note="pMB1 ori"
regulatory complement(2173..2200)
/note="T2 terminator"
/regulatory_class="terminator"
regulatory 2332..2375
/note="T1 terminator"
/regulatory_class="terminator"
regulatory complement(2529..2653)
/note="attR2"
/regulatory_class="attenuator"
misc_feature 2711..3291
/note="5' UTR of the EMCV polyprotein gene (IRES)"
CDS 3321..>4037
/transl_table=11
/note="EGFP"
/protein_id="SJL86744.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
CDS <4062..5711
/codon_start=1
/transl_table=11
/note="LUCm"
/protein_id="SJL86745.1"
/translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVA
PANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGF
QSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRF
SHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSL
QDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPG
IRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVR
GPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAP
AELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTA
KKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
regulatory 5727..5818
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 5819..6175
/note="3' flanking region of rabbit beta-globin"
BASE COUNT 1597 a 1542 c 1536 g 1598 t
ORIGIN
1 tcgagatatc tagcaacttt attatacaaa gttggcatta taaaaaagca ttgcttatca
61 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg gagctccatg
121 gtagcgttaa cgcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt
181 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
241 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
301 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
361 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
421 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
481 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
541 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
601 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
661 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
721 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
781 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
841 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
901 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
961 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
1021 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
1081 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
1141 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
1201 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
1261 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
1321 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
1381 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
1441 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
1501 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
1561 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
1621 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa
1681 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
1741 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
1801 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
1861 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
1921 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
1981 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
2041 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
2101 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt
2161 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
2221 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
2281 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
2341 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
2401 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
2461 taagctcggg ccctgcagct ctagagctcg aattctacag gtcactaata ccatctaagt
2521 agttgattca tagtgactgc atatgttgtg ttttacagta ttatgtagtc tgttttttat
2581 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcaactttct
2641 tgtacaaagt ggttaaagga accaattcag tcgaggtcga cggtatcgat aagcttgata
2701 tcgaattccg cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct
2761 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
2821 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
2881 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
2941 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
3001 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
3061 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
3121 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
3181 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
3241 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
3301 accatggtat ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
3361 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
3421 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
3481 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
3541 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
3601 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
3661 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
3721 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
3781 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
3841 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
3901 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
3961 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
4021 tggacgagct gtacaagtac tcagatccac cggtcgccac cgaagacgcc aaaaacataa
4081 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata
4141 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
4201 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga
4261 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
4321 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
4381 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg
4441 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa
4501 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
4561 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata
4621 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg
4681 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg
4741 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
4801 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
4861 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg
4921 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
4981 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg
5041 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag
5101 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
5161 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag
5221 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga
5281 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
5341 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc
5401 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag
5461 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
5521 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
5581 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
5641 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga
5701 tcgccgtgta attctagagc ggccgcgatc tttttccctc tgccaaaaat tatggggaca
5761 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
5821 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
5881 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
5941 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
6001 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
6061 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
6121 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
6181 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaagggcgaa
6241 ttctgcagat atccatcaca ctggcggccg cac
//