LOCUS LT726874 7613 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian vector pCOIN-DV, complete sequence. ACCESSION LT726874 VERSION LT726874.1 KEYWORDS . SOURCE Mammalian vector pCOIN-DV ORGANISM Mammalian vector pCOIN-DV other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7613) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7613 /organism="Mammalian vector pCOIN-DV" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 8192." /db_xref="taxon:1945165" misc_feature 35..277 /note="chicken beta-globin 5'HS4 insulator core sequence" misc_feature 278..520 /note="chicken beta-globin 5'HS4 insulator core sequence" regulatory 539..1046 /note="mPGK1 promoter" /regulatory_class="promoter" misc_feature 1070..1117 /note="FRT mutant" regulatory complement(1205..1288) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(1380..1782) /note="pMB1 ori" CDS complement(2323..3183) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL86740.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" misc_feature 3801..3848 /note="FRT" misc_feature 3865..3968 /note="SA" misc_feature complement(4031..4064) /note="loxP" regulatory 4751..4847 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(4751..4847) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 5005..5101 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(5005..5101) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 5259..5355 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(5259..5355) /note="SV40 polyA late" /regulatory_class="terminator" misc_feature complement(5444..5477) /note="loxP" regulatory complement(5575..5699) /note="attR4" /regulatory_class="attenuator" CDS complement(5740..6045) /transl_table=11 /note="ccdB" /protein_id="SJL86741.1" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD KVPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" CDS complement(6387..7046) /transl_table=11 /note="chloramphenicol resistance" /protein_id="SJL86742.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" regulatory complement(7095..7147) /note="lacUV5 promoter" /regulatory_class="promoter" regulatory 7155..7279 /note="attR3" /regulatory_class="attenuator" regulatory 7344..7568 /note="BGH polyA" /regulatory_class="terminator" BASE COUNT 1878 a 1967 c 1810 g 1956 t ORIGIN 1 cgcgccgggc cctcgcgact cgaggaataa attcgagctc acggggacag ccccccccca 61 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg 121 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc 181 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg 241 agcctgcaga cacctggggg atacggggaa aaagcttgag ctcacgggga cagccccccc 301 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc 361 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac 421 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct 481 ttgagcctgc agacacctgg gggatacggg gaaaaagctt gtcgacgaat aaattcaatt 541 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 601 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 661 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 721 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 781 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 841 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 901 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 961 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 1021 ctcttcctca tctccgggcc tttcgacctg caatcgccgc caccatggag aagttcctat 1081 tccgaagttc ctattcttca aaaggtatag gaacttccga tcgggatccc caatcctgca 1141 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 1201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 1261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 1321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 1381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 1441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 1501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 1561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 1621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 1681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 1741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 1801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 1861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 1921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 1981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 2041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 2101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 2161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 2221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 2281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 2341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 2401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 2461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 2521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 2581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 2641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 2701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 2761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 2821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 2881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 2941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 3001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 3061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 3121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 3181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 3241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 3301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 3361 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 3541 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 3601 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 3661 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 3721 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 3781 attccacaaa agtgcgatcg gaagttccta ttccgaagtt cctattctct agaaagtata 3841 ggaacttctt aattaaggga tctgtagggc gcagtagtcc agggtttcct tgatgatgtc 3901 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc 3961 tttccagtgg ggatcgacgg tatcgtagag tcgaggccgc tctagaacta gtggatccgg 4021 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg 4081 caggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta 4141 gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 4201 ccaccggtag cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 4261 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 4321 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 4381 ggtaggatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc 4441 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt 4501 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca 4561 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat 4621 cagaagcttg cagatctgcg actctagagg atctgcgact ctagaggatc ataatcagcc 4681 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 4741 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 4801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 4861 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctgc gactctagag 4921 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 4981 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 5041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 5101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 5161 ctgcgactct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta 5221 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt 5281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 5341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 5401 tatcatgtct ggatccccat caagctgatc cggaaccctt aatataactt cgtataatgt 5461 atgctatacg aagttattag gtccctcgac ctgcagccca agctagctta tcgataccgt 5521 cgacnctcga gnttttccca gtcacgacgt tgtaaaacga cggccagtga attatcaact 5581 ttgtatagaa aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag 5641 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg 5701 tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag 5761 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat 5821 aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat 5881 atgcaccacc gggtaaagtt cacgggggac tttatctgac agcagacgtg cactggccag 5941 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag 6001 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt 6061 tctcgtcggc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct 6121 gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc 6181 ttaccgaacc ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc 6241 aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtatac 6301 atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata ctgttatctg 6361 gcttttagta agccggatcc tctagattac gccccgccct gccactcatc gcagtactgt 6421 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga 6481 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg 6541 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag 6601 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt 6661 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg 6721 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg 6781 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga 6841 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc 6901 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga 6961 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg 7021 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga 7081 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg 7141 cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt 7201 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa 7261 ctttattata catagttgat aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa 7321 acgagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 7381 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 7441 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 7501 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 7561 ctctatggct tctgaggcgg aaagaaccag ctggggctcg atcctctagt tgg //