LOCUS LT726873 6228 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian vector Cre-excised pCOIN DV, complete sequence.
ACCESSION LT726873
VERSION LT726873.1
KEYWORDS .
SOURCE Mammalian vector Cre-excised pCOIN DV
ORGANISM Mammalian vector Cre-excised pCOIN DV
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6228)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6228
/organism="Mammalian vector Cre-excised pCOIN DV"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 8191."
/db_xref="taxon:1945163"
misc_feature 35..277
/note="chicken beta-globin 5'HS4 insulator core sequence"
misc_feature 278..520
/note="chicken beta-globin 5'HS4 insulator core sequence"
regulatory 539..1046
/note="mPGK1 promoter"
/regulatory_class="promoter"
misc_feature 1070..1117
/note="FRT mutant"
regulatory complement(1205..1288)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(1380..1782)
/note="pMB1 ori"
CDS complement(2323..3183)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86737.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 3801..3848
/note="FRT"
misc_feature 3865..3968
/note="SA"
misc_feature complement(4031..4064)
/note="loxP"
regulatory complement(4162..4286)
/note="attR4"
/regulatory_class="attenuator"
CDS complement(4327..4632)
/transl_table=11
/note="ccdB"
/protein_id="SJL86738.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(4974..5633)
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL86739.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
regulatory complement(5682..5735)
/note="lacUV5 promoter"
/regulatory_class="promoter"
regulatory 5743..5867
/note="attR3"
/regulatory_class="attenuator"
regulatory 5959..6183
/note="BGH polyA"
/regulatory_class="terminator"
BASE COUNT 1489 a 1626 c 1569 g 1542 t
ORIGIN
1 cgcgccgggc cctcgcgact cgaggaataa attcgagctc acggggacag ccccccccca
61 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg
121 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc
181 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg
241 agcctgcaga cacctggggg atacggggaa aaagcttgag ctcacgggga cagccccccc
301 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc
361 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac
421 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct
481 ttgagcctgc agacacctgg gggatacggg gaaaaagctt gtcgacgaat aaattcaatt
541 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
601 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
661 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
721 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
781 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
841 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
901 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
961 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
1021 ctcttcctca tctccgggcc tttcgacctg caatcgccgc caccatggag aagttcctat
1081 tccgaagttc ctattcttca aaaggtatag gaacttccga tcgggatccc caatcctgca
1141 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
1201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
1261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
1321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
1381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
1441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
1501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
1561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
1621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
1681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
1741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
1801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
1861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
1921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
1981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
2041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
2101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
2161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
2221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
2281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
2341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
2401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
2461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
2521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
2581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
2641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
2701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
2761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
2821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
2881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
2941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
3001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
3061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
3121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
3181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
3241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
3301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
3361 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
3541 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
3601 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
3661 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
3721 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
3781 attccacaaa agtgcgatcg gaagttccta ttccgaagtt cctattctct agaaagtata
3841 ggaacttctt aattaaggga tctgtagggc gcagtagtcc agggtttcct tgatgatgtc
3901 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc
3961 tttccagtgg ggatcgacgg tatcgtagag tcgaggccgc tctagaacta gtggatccgg
4021 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg
4081 cagcccaagc tagcttatcg ataccgtcga cnctcgagnt tttcccagtc acgacgttgt
4141 aaaacgacgg ccagtgaatt atcaactttg tatagaaaag ttgaacgaga aacgtaaaat
4201 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
4261 aaaacacaac atatccagtc actatggtcg acctgcagac tggctgtgta taagggagcc
4321 tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg
4381 gctgagatca gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt
4441 catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac gggggacttt
4501 atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat
4561 aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac
4621 cttaaactgc atttcaccag cccctgttct cgtcggcaaa agagccgttc atttcaataa
4681 accgggcgac ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac
4741 gacgggcttc attctgcatg gttgtgctta ccgaaccgga gatattgaca tcatatatgc
4801 cttgagcaac tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata
4861 cctctttttg acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca
4921 gcgcgcaaat acgcatactg ttatctggct tttagtaagc cggatcctct agattacgcc
4981 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
5041 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
5101 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
5161 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
5221 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
5281 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
5341 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
5401 ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
5461 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
5521 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
5581 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
5641 tccttagctc ctgaaaatct cgacggatcc taactcaaaa tccacacatt atacgagccg
5701 gaagcataaa gtgtaaagcc tgggggtgcc taatgcggcc gccatagtga ctggatatgt
5761 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
5821 tttatatcat tttacgtttc tcgttcaact ttattataca tagttgatag cttgatagct
5881 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc gggccgcgag
5941 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
6001 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
6061 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
6121 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
6181 tggcttctga ggcggaaaga accagctggg gctcgatcct ctagttgg
//