LOCUS LT726871 6245 bp DNA circular SYN 06-FEB-2017 DEFINITION Shuttle vector pAM1, complete sequence. ACCESSION LT726871 VERSION LT726871.1 KEYWORDS . SOURCE Shuttle vector pAM1 ORGANISM Shuttle vector pAM1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6245) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6245 /organism="Shuttle vector pAM1" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 8058." /db_xref="taxon:1945223" CDS complement(499..1233) /transl_table=11 /note="erythromycin resistance" /protein_id="SJL86727.1" /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF TLELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNI PYNISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPR EYFHPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH AGIDDLNNISFEQFLSLFNSYKLFNK" CDS 1698..1982 /transl_table=11 /note="copG-like" /protein_id="SJL86728.1" /translation="MSDFSERDRELLAMFGMGEDQVRDDVERMESETAGHGITGPVHY ALHMLPEHGEEMVSMTVKLPKSQLEKVTETARRYHISRSEYVRRQLAGAV" CDS complement(2174..2464) /transl_table=11 /note="orfX-like" /protein_id="SJL86729.1" /translation="MTIQTIRKKRPLPAKELAAMYDVSVRTIQRWASQTRKDWIDEQA TLRESIRAYHDDEGHTWPQTAEHFGMSQDAVRSRCYRARKERAAEAKAARPE" CDS complement(2461..3408) /transl_table=11 /note="repB" /protein_id="SJL86730.1" /translation="MSDEYSQPTLELSRTFEGWWLPRRPLCCDDDYSQLVRRSRTDAL RCKHIEANPSALVNTIVVDIDDANAKAMALWGHRGMLPNWIAENPANGHAHAGWVLTY PVPRTDMARLKPLKLLHAVTEGLRRSVDGDEGYSGLLMKNPLSDAWDSDLCREDTYDL PDLVAALEEHGDMPPKSWTRTKRAREVGVGRNCTLFDEARTLAYRQVRRLPDRTPASS DLLREYVRRTCHEINASFPDPLPVREVNDTAKSIHKWITTRSRMWRDGAVANAATFVA IQSARGRKGGRGNKRDKQGNVQNAFKQKAELFGKEMMGQ" misc_feature complement(4029..4066) /note="5' UTR of lacZ, incl. RBS" regulatory complement(4067..4150) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(4242..4644) /note="pMB1 ori" CDS complement(5185..6045) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL86731.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1447 a 1604 c 1645 g 1543 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccnnngtc 421 gacaaaagac ataatcgatt cacaaaaaat aggcacacga aaaacaagtt aagggatgca 481 gtttatgcat cccttaactt acttattaaa taatttatag ctattgaaaa gagataagaa 541 ttgttcaaag ctaatattgt ttaaatcgtc aattcctgca tgttttaagg aattgttaaa 601 ttgatttttt gtaaatattt tcttgtattc tttgttaacc catttcataa cgaaataatt 661 atacttttgt ttatctttgt gtgatattct tgattttttt ctacttaatc tgataagtga 721 gctattcact ttaggtttag gatgaaaata ttctcttgga accatactta atatagaaat 781 atcaacttct gccattaaaa gtaatgccaa tgagcgtttt gtatttaata atcttttagc 841 aaacccgtat tccacgatta aataaatctc attagctata ctatcaaaaa caattttgcg 901 tattatatcc gtacttatgt tataaggtat attaccatat attttatagg attggttttt 961 aggaaattta aactgcaata tatccttgtt taaaacttgg aaattatcgt gatcaacaag 1021 tttattttct gtagttttgc ataatttatg gtctatttca atggcagtta cgaaattaca 1081 cctctttact aattcaaggg taaaatggcc ttttcctgag ccgatttcaa agatattatc 1141 atgttcattt aatcttatat ttgtcattat tttatctata ttatgttttg aagtaataaa 1201 gttttgactg tgttttatat ttttctcgtt cattataacc ctctttaatt tggttatatg 1261 aattttgctt attaacgatt cattataacc acttattttt tgtttggttg ataatgaact 1321 gtgctgatta caaaaatact aaaaatgccc atattttttc ctccttataa aattagtata 1381 attatagcac gagctctgat aaatatgaac atgatgagtg atcgttaacn nngggggatc 1441 tacgtgctgc cgcgcgtgca tgagcggcat cccgagctgt ccgtggagga cgtgcttgcg 1501 gcgttcagga gcgtcatggt ggacgcccgc agggacaacg gcacgtgggt ctgcatcggc 1561 ctcgacgggc gcggcaggga cgtggagatg gtctacgagc agcgcggcga ccgcgcgctc 1621 atctaccacg ccatgacgcc gccgacgaag aagacccgaa gcgagatagc ccgcctgcgc 1681 gggggaagga gcgaccgatg agcgatttca gcgaacgcga cagggagctg ttggcgatgt 1741 tcggcatggg cgaggatcag gtcagggacg acgtggagcg catggagtcc gagaccgcgg 1801 gccacggcat aaccggaccc gtccactacg cgctgcacat gctccccgag cacggcgagg 1861 agatggtcag catgaccgtc aagctgccca agagtcagct ggagaaggtc acggagaccg 1921 ccaggcgcta ccacatcagc cgcagcgagt acgtgcgccg gcagctcgcc ggagccgtct 1981 gaccgccgcc gcgcgaagac ggaacacccg tgtgccatca ttcgtggcac acgggtgttc 2041 ccataggtga caccgcggtg tcaccatgcc gcgcgagcgg tctggaggcg cagccgacca 2101 tggggtacgg gtcttcccgg caagccgatt cgcggtgcga atcgagtgaa cgtatatacg 2161 ttcacgtcac ttgctactcc ggccttgcgg cttttgcctc ggccgcacgc tccttgcgcg 2221 cgcggtagca gcggctacgc accgcgtctt ggctcatgcc gaagtgctcg gcggtctgcg 2281 gccacgtatg gccctcgtca tcgtggtagg cacggatgga ttcgcgcaac gttgcctgtt 2341 cgtctatcca gtccttacgg gtttgtgaag cccatcgctg aatagtgcgc acggagacgt 2401 cgtacattgc cgccagctcc ttggcgggaa gcggacgctt cttgcgaatc gtctgaatcg 2461 tcattgcccc atcatctcct tgccgaacag ctctgccttt tgcttgaagg cattttgaac 2521 gttcccttgc ttgtcgcgtt tgtttccgcg accgcctttg cgtccgcgcg cggattggat 2581 ggcgacgaac gtggcggcgt tggcgacggc accgtccctc cacatgcggc ttcgcgtggt 2641 gatccacttg tggatgctct tggcggtgtc gttgacctcg cgcacgggca gcgggtcggg 2701 gaacgaggcg ttgatttcgt ggcaggtgcg gcgcacgtac tcgcgcagca ggtcggagga 2761 ggcgggcgtg cggtcgggca gtcggcgcac ctgacggtag gcgagggtgc gggcctcgtc 2821 gaacaaggtg cagttgcggc ccacgccgac ctcgcgggcg cgtttggtgc gcgtccagct 2881 cttgggcggc atgtccccgt gctcttcgag cgcggccacg aggtcgggca ggtcgtaggt 2941 gtcctcgcgg cacaggtcgc tgtcccacgc gtcgctcagc gggttcttca tcagcaggcc 3001 ggaatagccc tcgtcgccgt ccacgctgcg gcgcagcccc tcggtgacgg cgtgcaggag 3061 cttcagcggt ttgagccggg ccatgtcggt gcggggcacg gggtaggtca gcacccagcc 3121 cgcgtgagcg tgcccgttgg ccgggttctc cgcgatccag ttcggcagca tcccacggtg 3181 cccccacagg gccatcgcct tggcgttcgc gtcgtcgatg tccaccacga tcgtgttcac 3241 cagcgccgag ggattcgcct cgatgtgctt gcatctgagc gcgtcggtgc ggctccggcg 3301 caccagctgg ctgtagtcgt cgtcgcagca cagcggacgc cggggcagcc accagccctc 3361 gaacgtgcgc gacagctcaa gcgtcggctg cgaatactcg tcagacatgg gcactagtgt 3421 acgtcgtact tgaccactgg tcaagttcgc ctaaaaccaa gtcagatatt tcagcccagc 3481 aaagcatggg ctgctccttc ccagagttac ccaaaactgt cgaaatctgg ccaaaaacac 3541 cccaaaccga acgatcatcc accctcatgc gcttcctatg cgctcagatg gatatatcca 3601 tggataccca catggacacg tctatggaca cccacatgga tatgtccatg gacatccaca 3661 tggagatgtc catcaaatcg aaggtaagca agctagacgg ttcatgtaca tgaaccgaac 3721 gcagcacctc ctgcgccctt cggcgcagga gcaacaaccc gaggccgagg aaaaagccgc 3781 aaggccggag tagcaagcga cgtgattgta gctacaatca ctcgattcgc ggtgcgaatc 3841 ggcttgccgg gaagacccgt accccatggt cggcggagcc tccagaccgc tcgcgcggct 3901 tgtgccacaa cggtgttcca cttgtgtgac cgtggtgtta ctctggtatc aactttgctt 3961 ggaggtgctg tttggagatc ctctagagtc gacctgcagg catgcaagct tggcgtaatc 4021 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 4081 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 4141 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 4201 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 4261 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 4321 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 4381 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 4441 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 4501 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 4561 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 4621 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 4681 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 4741 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 4801 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 4861 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 4921 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 4981 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 5041 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 5101 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 5161 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 5221 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 5281 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 5341 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 5401 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 5461 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 5521 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 5581 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 5641 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 5701 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 5761 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 5821 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 5881 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 5941 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 6001 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 6061 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 6121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 6181 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 6241 tcgtc //