LOCUS LT726868 5374 bp DNA circular SYN 06-FEB-2017
DEFINITION Diatom vector pDEST-N-HA, complete sequence.
ACCESSION LT726868
VERSION LT726868.1
KEYWORDS .
SOURCE Diatom vector pDEST-N-HA
ORGANISM Diatom vector pDEST-N-HA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5374)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5374
/organism="Diatom vector pDEST-N-HA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 7881."
/db_xref="taxon:1944723"
rep_origin complement(6..461)
/note="f1 ori"
regulatory 626..642
/note="T7 promoter"
/regulatory_class="promoter"
regulatory 865..1100
/note="FcpB promoter"
/regulatory_class="promoter"
misc_feature 1114..1140
/note="HA epitope"
misc_feature 1141..1167
/note="HA epitope"
misc_feature 1183..1209
/note="HA epitope"
regulatory complement(1225..1349)
/note="attR1"
/regulatory_class="attenuator"
regulatory 1357..1409
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 1458..2117
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL86720.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 2459..2764
/transl_table=11
/note="ccdB"
/protein_id="SJL86721.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 2805..2929
/note="attR2"
/regulatory_class="attenuator"
regulatory 3097..3170
/note="FcpA terminator"
/regulatory_class="terminator"
regulatory complement(3191..3207)
/note="T3 promoter"
/regulatory_class="promoter"
misc_feature complement(3233..3270)
/note="lacZ RBS"
regulatory complement(3271..3354)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(3446..3848)
/note="pMB1 ori"
CDS complement(4389..5249)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86722.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1411 a 1275 c 1319 g 1369 t
ORIGIN
1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
661 ccgcggaatc tcgcccattc atggtgtata caagttcaac atccaaagct ggaacttttg
721 gaaacagaaa gaatgtccga atagggcacg gcgtgccgta ttgttggagt ggactagcag
781 aaagtgagga aggcacagga tgagtttcct cgagacacat agcttcagcg tcgtgtaggc
841 taggcagagg tgagttttct cgagacatac cttcagcgtc gtcttcactg tcacagtcaa
901 ctgacagtaa tcgttgatcc ggagagattc aaaattcaat ctgtttggac ctggataaga
961 cacaagagcg acatcctgac atgaacgccg taaacagcaa atcctggttg aacacgtatc
1021 cttttggggg cctccagcta cgacgctcgc cccagctggg gcttccttac tatacacagc
1081 gcatatttca cggttgccag cggccgcatg cgatacccct acgacgtgcc cgactacgcc
1141 tacccctacg acgtgcccga ctacgccgat cgatccggac cgtaccccta cgacgtgccc
1201 gactacgccg ctggatcccc catcacaagt ttgtacaaaa aagctgaacg agaaacgtaa
1261 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
1321 tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac
1381 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
1441 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
1501 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
1561 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
1621 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
1681 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
1741 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
1801 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
1861 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
1921 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
1981 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
2041 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
2101 ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt
2161 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
2221 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
2281 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
2341 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
2401 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
2461 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
2521 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
2581 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
2641 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
2701 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
2761 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
2821 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
2881 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtg atgggctgca
2941 ggaattccga gctacctcga ctttggctgg gacactttca gtgaggacaa gaagcttcag
3001 aagcgtgcta tcgaactcaa ccagggacgt gcggcacaaa tgggcatcct tgctctcatg
3061 gtgcacgaac agttgggagt ctctatcctt ccttaaaaat ttaattttca ttagttgcag
3121 tcactccgct ttggtttcac agtcaggaat aacactagct cgtcttcacc ggtacccagc
3181 ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt
3241 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
3301 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
3361 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
3421 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
3481 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
3541 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
3601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
3661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
3721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
3781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
3841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
3901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
3961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
4021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
4081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
4141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
4201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
4261 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
4321 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
4381 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
4441 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
4501 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
4561 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
4621 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
4681 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
4741 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
4801 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
4861 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
4921 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
4981 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
5041 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
5101 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
5161 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
5221 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
5281 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
5341 ataggggttc cgcgcacatt tccccgaaaa gtgc
//