LOCUS LT726862 5843 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCR3-pro-IL-1B-D108A, complete
sequence.
ACCESSION LT726862
VERSION LT726862.1
KEYWORDS .
SOURCE Mammalian expression vector pCR3-pro-IL-1B-D108A
ORGANISM Mammalian expression vector pCR3-pro-IL-1B-D108A
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5843)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5843
/organism="Mammalian expression vector
pCR3-pro-IL-1B-D108A"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 7534."
/db_xref="taxon:1944962"
regulatory 10..593
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
regulatory 638..654
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 691..723
/note="VSV epitope"
CDS 736..1545
/transl_table=11
/note="mIL1B mutant"
/protein_id="SJL86700.1"
/translation="MATVPELNCEMPPFDSDENDLFFEVDGPQKMKGCFQTFDLGCPD
ESIQLQISQQHINKSFRQAVSLIVAVEKLWQLPVSFPWTFQDEDMSTFFSFIFEEEPI
LCDSWADDDNLLVCDVPIRQLHYGLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFS
MSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVF
NKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS"
misc_feature 1057..1059
/note="D108A"
misc_feature 1111..1113
/note="R126G"
regulatory complement(1560..1576)
/note="SP6 promoter"
/regulatory_class="promoter"
regulatory 1602..1826
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin complement(1850..2176)
/note="pMB1 ori"
regulatory complement(2894..2963)
/note="HSV-TK polyA"
/regulatory_class="terminator"
CDS complement(3154..3942)
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL86701.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH
QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
rep_origin complement(4002..4077)
/note="SV40 ori"
regulatory complement(4034..4315)
/note="SV40 early promoter"
/regulatory_class="promoter"
CDS complement(4394..5254)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86702.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 5386..5841
/note="f1 ori"
BASE COUNT 1423 a 1523 c 1478 g 1416 t
ORIGIN
1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
661 cccaagcttg gtaccggatc cgccaccatg tacaccgaca tcgagatgaa ccggttgggc
721 aaggaattgg gtcagatggc aactgttcct gaactcaact gtgaaatgcc accttttgac
781 agtgatgaga atgacctgtt ctttgaagtt gacggacccc aaaagatgaa gggctgcttc
841 caaacctttg acctgggctg tcctgatgag agcatccagc ttcaaatctc gcagcagcac
901 atcaacaaga gcttcaggca ggcagtatca ctcattgtgg ctgtggagaa gctgtggcag
961 ctacctgtgt ctttcccgtg gaccttccag gatgaggaca tgagcacctt cttttccttc
1021 atctttgaag aagagcccat cctctgtgac tcatgggctg atgatgataa cctgctggtg
1081 tgtgacgttc ccattagaca actgcactac gggctccgag atgaacaaca aaaaagcctc
1141 gtgctgtcgg acccatatga gctgaaagct ctccacctca atggacagaa tatcaaccaa
1201 caagtgatat tctccatgag ctttgtacaa ggagaaccaa gcaacgacaa aatacctgtg
1261 gccttgggcc tcaaaggaaa gaatctatac ctgtcctgtg taatgaaaga cggcacaccc
1321 accctgcagc tggagagtgt ggatcccaag caatacccaa agaagaagat ggaaaaacgg
1381 tttgtcttca acaagataga agtcaagagc aaagtggagt ttgagtctgc agagttcccc
1441 aactggtaca tcagcacctc acaagcagag cacaagcctg tcttcctggg aaacaacagt
1501 ggtcaggaca taattgactt caccatggaa tctgtgtctt cctaannngg gccctattct
1561 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
1621 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
1681 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
1741 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
1801 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagtg gcggtaatac
1861 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
1921 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
1981 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
2041 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
2101 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
2161 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
2221 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
2281 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
2341 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
2401 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
2461 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
2521 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
2581 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
2641 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
2701 aacctgaggc tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac
2761 ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg
2821 gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa
2881 acgacccaac accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt
2941 ccggtattgt ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca
3001 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
3061 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg
3121 cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
3181 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
3241 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
3301 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
3361 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct
3421 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt gatcatcctg
3481 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
3541 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
3601 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
3661 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
3721 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc
3781 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
3841 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
3901 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
3961 tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct
4021 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
4081 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
4141 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
4201 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
4261 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctggt
4321 tctttccgcc tcaggactct tcctttttca ataaatcaat ctaaagtata tatgagtaaa
4381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
4441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
4501 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
4561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
4621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
4681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
4741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
4801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
4861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
4921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
4981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
5041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
5101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
5161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
5221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
5281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
5341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
5401 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
5461 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
5521 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
5581 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
5641 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
5701 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
5761 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
5821 aaatattaac gcttacaatt tac
//