LOCUS LT726859 5969 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAG-hCASP-1-C285A, complete
sequence.
ACCESSION LT726859
VERSION LT726859.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAG-hCASP-1-C285A
ORGANISM Mammalian expression vector pCAGGS-FLAG-hCASP-1-C285A
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5969)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5969
/organism="Mammalian expression vector
pCAGGS-FLAG-hCASP-1-C285A"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 7424."
/db_xref="taxon:1944828"
misc_feature 13..39
/note="FLAG epitope"
CDS 46..1260
/transl_table=11
/note="hCASP1 mutant"
/protein_id="SJL86693.1"
/translation="MADKVLKEKRKLFIRSMGEGTINGLLDELLQTRVLNKEEMEKVK
RENATVMDKTRALIDSVIPKGAQACQICITYICEEDSYLAGTLGLSADQTSGNYLNMQ
DSQGVLSSFPAPQAVQDNPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSS
RTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAF
AHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDK
PKVIIIQAARGDSPGVVWFKDSVGVSGNLSLPTTEEFEDDAIKKAHIEKDFIAFCSST
PDNVSWRHPTMGSVFIGRLIEHMQEYACSCDVEEIFRKVRFSFEQPDGRAQMPTTERV
TLTRCFYLFPGH"
misc_feature 898..900
/note="C285A"
regulatory 1262..1353
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1354..1710
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1788..1870)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2012..2087
/note="SV40 ori"
regulatory 2118..2192
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2118..2192)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2307..2708)
/note="pMB1 ori"
CDS complement(3249..4109)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86694.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4245..4622
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4628..5969
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4997..5921
/note="intron AG promoter"
BASE COUNT 1369 a 1516 c 1610 g 1474 t
ORIGIN
1 gaattcctcg agatggacta caaggacgac gatgacaagg gtaccatggc cgacaaggtc
61 ctgaaggaga agagaaagct gtttatccgt tccatgggtg aaggtacaat aaatggctta
121 ctggatgaat tattacagac aagggtgctg aacaaggaag agatggagaa agtaaaacgt
181 gaaaatgcta cagttatgga taagacccga gctttgattg actccgttat tccgaaaggg
241 gcacaggcat gccaaatttg catcacatac atttgtgaag aagacagtta cctggcaggg
301 acgctgggac tctcagcaga tcaaacatct ggaaattacc ttaatatgca agactctcaa
361 ggagtacttt cttcctttcc agctcctcag gcagtgcagg acaacccagc tatgcccaca
421 tcctcaggct cagaagggaa tgtcaagctt tgctccctag aagaagctca aaggatatgg
481 aaacaaaagt cggcagagat ttatccaata atggacaagt caagccgcac acgtcttgct
541 ctcattatct gcaatgaaga atttgacagt attcctagaa gaactggagc tgaggttgac
601 atcacaggca tgacaatgct gctacaaaat ctggggtaca gcgtagatgt gaaaaaaaat
661 ctcactgctt cggacatgac tacagagctg gaggcatttg cacaccgccc agagcacaag
721 acctctgaca gcacgttcct ggtgttcatg tctcatggta ttcgggaagg catttgtggg
781 aagaaacact ctgagcaagt cccagatata ctacaactca atgcaatctt taacatgttg
841 aataccaaga actgcccaag tttgaaggac aaaccgaagg tgatcatcat ccaggccgcc
901 cgtggtgaca gccctggtgt ggtgtggttt aaagattcag taggagtttc tggaaaccta
961 tctttaccaa ctacagaaga gtttgaggat gatgctatta agaaagccca catagagaag
1021 gattttatcg ctttctgctc ttccacacca gataatgttt cttggagaca tcccacaatg
1081 ggctctgttt ttattggaag actcattgaa catatgcaag aatatgcctg ttcctgtgat
1141 gtggaggaaa ttttccgcaa ggttcgattt tcatttgagc agccagatgg tagagcgcag
1201 atgcccacca ctgaaagagt gactttgaca agatgtttct acctcttccc aggacattaa
1261 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
1321 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
1381 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
1441 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
1501 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
1561 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
1621 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
1681 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
1741 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
1801 gagccggaag cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat
1861 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat
1921 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
1981 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
2041 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
2101 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
2161 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
2221 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc
2281 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
2341 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
2401 cacagaatca gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
2461 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
2521 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
2581 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
2641 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
2701 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
2761 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
2821 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
2881 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
2941 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
3001 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
3061 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
3121 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
3181 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
3241 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
3301 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
3361 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
3421 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
3481 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
3541 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
3601 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
3661 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
3721 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
3781 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
3841 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
3901 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
3961 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
4021 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
4081 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
4141 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
4201 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac
4261 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
4321 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
4381 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
4441 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
4501 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
4561 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
4621 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
4681 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
4741 gggggggccc cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa
4801 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
4861 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg
4921 ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
4981 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
5041 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
5101 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
5161 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
5221 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
5281 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
5341 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
5401 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
5461 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
5521 ggagggctcg ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
5581 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
5641 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
5701 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
5761 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
5821 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
5881 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
5941 ttattgtgct gtctcatcat tttggcaaa
//