LOCUS LT726858 5363 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-E-ASC, complete sequence. ACCESSION LT726858 VERSION LT726858.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-E-ASC ORGANISM Mammalian expression vector pCAGGS-E-ASC other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5363) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5363 /organism="Mammalian expression vector pCAGGS-E-ASC" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 7414." /db_xref="taxon:1944760" misc_feature 19..57 /note="E-tag" CDS 67..654 /transl_table=11 /note="hPYCARD" /protein_id="SJL86691.1" /translation="MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGAL LSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQA PPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMR KLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS" regulatory 656..747 /note="3' UTR of rabbit beta-globin, incl. polyA" /regulatory_class="terminator" misc_feature 748..1104 /note="3' flanking region of rabbit beta-globin" regulatory complement(1182..1264) /note="lac promoter" /regulatory_class="promoter" rep_origin 1406..1481 /note="SV40 ori" regulatory 1512..1586 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(1512..1586) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin complement(1701..2102) /note="pMB1 ori" CDS complement(2643..3503) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL86692.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 3639..4016 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 4022..5363 /note="AG promoter" /regulatory_class="promoter" misc_feature 4391..5315 /note="intron AG promoter" BASE COUNT 1105 a 1457 c 1529 g 1272 t ORIGIN 1 gaattgaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg 61 gccgctatgg ggcgcgcgcg cgacgccatc ctggatgcgc tggagaacct gaccgccgag 121 gagctcaaga agttcaagct gaagctgctg tcggtgccgc tgcgcgaggg ctacgggcgc 181 atcccgcggg gcgcgctgct gtccatggac gccttggacc tcaccgacaa gctggtcagc 241 ttctacctgg agacctacgg cgccgagctc accgctaacg tgctgcgcga catgggcctg 301 caggagatgg ccgggcagct gcaggcggcc acgcaccagg gctctggagc cgcgccagct 361 gggatccagg cccctcctca gtcggcagcc aagccaggcc tgcactttat agaccagcac 421 cgggctgcgc ttatcgcgag ggtcacaaac gttgagtggc tgctggatgc tctgtacggg 481 aaggtcctga cggatgagca gtaccaggca gtgcgggccg agcccaccaa cccaagcaag 541 atgcggaagc tcttcagttt cacaccagcc tggaactgga cctgcaagga cttgctcctc 601 caggccctaa gggagtccca gtcctacctg gtggaggacc tggagcggag ctgaagatct 661 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 721 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 781 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag 841 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca 901 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg 961 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt 1021 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc 1081 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt 1141 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 1201 gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt 1261 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat 1321 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 1381 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 1441 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 1501 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 1561 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 1621 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag 1681 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 1741 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 1801 atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 1861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 1921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 1981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 2041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 2101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 2161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 2221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 2281 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 2341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 2401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 2461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 2521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 2581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 2641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 2701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 2761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 2821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 2881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 2941 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 3001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 3061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 3121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 3181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 3241 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 3301 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 3361 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 3421 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 3481 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 3541 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 3601 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta 3661 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 3721 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 3781 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 3841 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac 3901 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 3961 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 4021 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat 4081 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg 4141 gcccccccca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc 4201 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg 4261 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc 4321 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt 4381 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt 4441 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg 4501 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc 4561 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg 4621 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg 4681 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg 4741 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca 4801 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg 4861 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg 4921 ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc 4981 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa 5041 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga 5101 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc 5161 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg 5221 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg 5281 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg 5341 tgctgtctca tcattttggc aaa //