LOCUS LT726854 7095 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF6-hbeclin-1-Myc-His, complete
sequence.
ACCESSION LT726854
VERSION LT726854.1
KEYWORDS .
SOURCE Mammalian expression vector pEF6-hbeclin-1-Myc-His
ORGANISM Mammalian expression vector pEF6-hbeclin-1-Myc-His
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7095)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7095
/organism="Mammalian expression vector
pEF6-hbeclin-1-Myc-His"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 7300."
/db_xref="taxon:1945001"
regulatory 386..1529
/note="hEF1a promoter"
/regulatory_class="promoter"
misc_feature 582..1520
/note="intron hEF1a promoter"
regulatory 1546..1562
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1597..>2946
/transl_table=11
/note="hBECN1"
/protein_id="SJL86680.1"
/translation="MEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTA
PLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGE
ASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQ
NYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAE
AERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHI
WHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYG
NHSYLESLTDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCL
PYRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQFYNK
"
misc_feature 2977..3006
/note="Myc epitope"
misc_feature 3022..3039
/note="His-tag"
regulatory 3068..3292
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 3338..3766
/note="f1 ori"
regulatory 3771..4052
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4009..4084
/note="SV40 ori"
regulatory 4157..4222
/note="synthetic EM7 promoter"
/regulatory_class="promoter"
CDS 4223..4621
/transl_table=11
/note="blasticidin resistance"
/protein_id="SJL86681.1"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
regulatory 4779..4853
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4779..4853)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(4992..5075)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(5167..5558)
/note="pMB1 ori"
CDS complement(6099..6959)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86682.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1669 a 1766 c 1902 g 1756 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
1561 tagggagacc caagctggct aggtaagctt ggtaccatgg aagggtctaa gacgtccaac
1621 aacagcacca tgcaggtgag cttcgtgtgc cagcgctgca gccagcccct gaaactggac
1681 acgagtttca agatcctgga ccgtgtcacc atccaggaac tcacagctcc attacttacc
1741 acagcccagg cgaaaccagg agagacccag gaggaagaga ctaactcagg agaggagcca
1801 tttattgaaa ctcctcgcca ggatggtgtc tctcgcagat tcatcccccc agccaggatg
1861 atgtccacag aaagtgccaa cagcttcact ctgattgggg aggcatctga tggcggcacc
1921 atggagaacc tcagccgaag actgaaggtc actggggacc tttttgacat catgtcgggc
1981 cagacagatg tggatcaccc actctgtgag gaatgcacag atactctttt agaccagctg
2041 gacactcagc tcaacgtcac tgaaaatgag tgtcagaact acaaacgctg tttggagatc
2101 ttagagcaaa tgaatgagga tgacagtgaa cagttacaga tggagctaaa ggagctggca
2161 ctagaggagg agaggctgat ccaggagctg gaagacgtgg aaaagaaccg caagatagtg
2221 gcagaaaatc tcgagaaggt ccaggctgag gctgagagac tggatcagga ggaagctcag
2281 tatcagagag aatacagtga atttaaacga cagcagctgg agctggatga tgagctgaag
2341 agtgttgaaa accagatgcg ttatgcccag acgcagctgg ataagctgaa gaaaaccaac
2401 gtctttaatg caaccttcca catctggcac agtggacagt ttggcacaat caataacttc
2461 aggctgggtc gcctgcccag tgttcccgtg gaatggaatg agattaatgc tgcttggggc
2521 cagactgtgt tgctgctcca tgctctggcc aataagatgg gtctgaaatt tcagagatac
2581 cgacttgttc cttacggaaa ccattcatat ctggagtctc tgacagacaa atctaaggag
2641 ctgccgttat actgttctgg ggggttgcgg tttttctggg acaacaagtt tgaccatgca
2701 atggtggctt tcctggactg tgtgcagcag ttcaaagaag aggttgagaa aggcgagact
2761 cgtttttgtc ttccctacag gatggatgtg gagaaaggca agattgaaga cacaggaggc
2821 agtggcggct cctattccat caaaacccag tttaactctg aggagcagtg gacaaaagct
2881 ctcaagttca tgctgacgaa tcttaagtgg ggtcttgctt gggtgtcctc acaattttat
2941 aacaaanngc ggccgctcga gtctagaggg cccttcgaac aaaaactcat ctcagaagag
3001 gatctgaata tgcataccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
3061 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
3121 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
3181 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
3241 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
3301 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
3361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
3421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
3481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
3541 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
3601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
3661 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
3721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
3781 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
3841 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
3901 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
3961 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
4021 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
4081 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
4141 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
4201 cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat
4261 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
4321 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
4381 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
4441 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
4501 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
4561 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
4621 agcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
4681 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
4741 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
4801 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
4861 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
4921 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
4981 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
5041 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
5101 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
5161 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
5221 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
5281 caggaaagaa catgtgagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
5341 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
5401 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
5461 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
5521 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
5581 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
5641 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
5701 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
5761 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
5821 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
5881 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
5941 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
6001 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
6061 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
6121 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
6181 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
6241 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
6301 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
6361 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
6421 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
6481 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
6541 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
6601 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
6661 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
6721 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
6781 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
6841 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
6901 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
6961 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
7021 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
7081 gtgccacctg acgtc
//