LOCUS LT726853 5263 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-hbeclin-1-N, complete
sequence.
ACCESSION LT726853
VERSION LT726853.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-hbeclin-1-N
ORGANISM Mammalian expression vector pCAGGS-E-hbeclin-1-N
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5263)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5263
/organism="Mammalian expression vector
pCAGGS-E-hbeclin-1-N"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 7299."
/db_xref="taxon:1944774"
misc_feature 16..54
/note="E-tag"
CDS 64..465
/transl_table=11
/note="hBECN1-N fragment"
/protein_id="SJL86678.1"
/translation="MEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTA
PLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGE
ASDGGTMENLSRRLKVTGDLFDIMSGQTDVD"
regulatory 553..644
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 645..1001
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1079..1161)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1303..1378
/note="SV40 ori"
regulatory complement(1409..1483)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1409..1483
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1598..1999)
/note="pMB1 ori"
CDS complement(2540..3400)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86679.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3536..3913
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3919..5260
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4288..5212
/note="intron AG promoter"
BASE COUNT 1120 a 1403 c 1467 g 1273 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gctatggaag ggtctaagac gtccaacaac agcaccatgc aggtgagctt cgtgtgccag
121 cgctgcagcc agcccctgaa actggacacg agtttcaaga tcctggaccg tgtcaccatc
181 caggaactca cagctccatt acttaccaca gcccaggcga aaccaggaga gacccaggag
241 gaagagacta actcaggaga ggagccattt attgaaactc ctcgccagga tggtgtctct
301 cgcagattca tccccccagc caggatgatg tccacagaaa gtgccaacag cttcactctg
361 attggggagg catctgatgg cggcaccatg gagaacctca gccgaagact gaaggtcact
421 ggggaccttt ttgacatcat gtcgggccag acagatgtgg attgagtcga ggaattcact
481 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
541 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
601 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
661 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
721 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
781 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
841 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
901 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
961 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
1021 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
1081 acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg agtgagctaa
1141 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
1201 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
1261 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
1321 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
1381 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa
1441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
1501 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc
1561 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
1621 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
1681 atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca aaaggccagc
1741 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
1801 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
1861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
1921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
1981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
2041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
2101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
2161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
2221 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
2281 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
2341 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
2401 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
2461 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
2521 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
2581 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
2641 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
2701 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
2761 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
2821 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
2881 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
2941 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
3001 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
3061 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
3121 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
3181 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
3241 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
3301 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
3361 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
3421 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
3481 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat
3541 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
3601 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
3661 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
3721 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
3781 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
3841 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
3901 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
3961 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
4021 gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg gcggggcggg
4081 gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
4141 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
4201 gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
4261 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
4321 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
4381 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
4441 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
4501 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
4561 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
4621 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
4681 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
4741 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
4801 cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg ccggcggctg
4861 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
4921 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
4981 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
5041 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
5101 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
5161 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
5221 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa
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