LOCUS LT726852 5342 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pAdD26SV(A)-3, complete sequence.
ACCESSION LT726852
VERSION LT726852.1
KEYWORDS .
SOURCE Mammalian expression vector pAdD26SV(A)-3
ORGANISM Mammalian expression vector pAdD26SV(A)-3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5342)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5342
/organism="Mammalian expression vector pAdD26SV(A)-3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 729."
/db_xref="taxon:1944748"
CDS 55..1245
/transl_table=11
/note="tetracycline resistance"
/protein_id="SJL86676.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
rep_origin complement(1400..1668)
/note="pMB1 ori"
regulatory complement(3129..3225)
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory 3129..3225
/note="SV40 polyA late"
/regulatory_class="terminator"
CDS complement(3390..3953)
/transl_table=11
/note="mDhfr"
/protein_id="SJL86677.1"
/translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSV
EGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIE
QPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLL
PEYPGVLSEVQEEKGIKYKFEVYEKKD"
misc_feature complement(4069..4317)
/note="hybrid intron late leader/immunoglobulin"
regulatory complement(4318..5101)
/note="Adenovirus 2 major late promoter"
/regulatory_class="promoter"
regulatory 5110..5282
/note="SV40 early promoter"
/regulatory_class="promoter"
regulatory complement(5110..5245)
/note="SV40 late promoter"
/regulatory_class="promoter"
rep_origin 5239..5314
/note="SV40 ori"
BASE COUNT 1233 a 1480 c 1357 g 1264 t
ORIGIN
1 ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa
61 tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc
121 ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc
181 agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt
241 ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga
301 gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga
361 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
421 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
481 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
541 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
601 gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
661 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
721 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
781 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
841 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
901 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
961 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
1021 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
1081 cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg
1141 gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc
1201 cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc
1261 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
1321 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
1381 gcacgcggcg catctcggga aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
1441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
1501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
1561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
1621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
1681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
1741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
1801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
1861 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
1921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
1981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
2041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
2101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
2161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
2221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
2281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
2341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
2401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
2461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
2521 tgctgcaggg tgtgttagca aactacagga ccaaatatcc tgctcaaact gtaaccccaa
2581 aaaatgctac agttgacagt cagcagatga acactgacca caaggctgtt ttggataagg
2641 ataatgctta tccagtggag tgctgggttc ctgatccaag taaaaatgaa aacactagat
2701 attttggaac ctacacaggt ggggaaaatg tgcctcctgt tttgcacatt actaacacag
2761 caaccacagt gcttcttgat gagcagggtg ttgggccctt gtgcaaagct gacagcttgt
2821 atgtttctgc tgttgacatt tgtgggctgt ttaccaacac ttctggaaca cagcagtgga
2881 agggacttcc cagatatttt aaaattaccc ttagaaagcg gtctgtgaaa aacccctacc
2941 caatttcctt tttgttaagt gacctaatta acaggaggac acagagggtg gatgggcagc
3001 ctatgattgg aatgtcctct caagtagagg aggttagggt ttatgaggac acagaggagc
3061 ttcctgggga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
3121 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
3181 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
3241 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
3301 attatgatct aaagccagca aaagtcccat ggtcttataa aaatgcatag ctttaggagg
3361 ggagcagaga acttgaaagc atcttcctgt tagtctttct tctcgtagac ttcaaactta
3421 tacttgatgc ctttttcctc ctggacctca gagaggacgc ctgggtattc tgggagaagt
3481 ttatatttcc ccaaatcaat ttctgggaaa aacgtgtcac tttcaaattc ctgcatgatc
3541 cttgtcacaa agagtctaag gtggcctggt tgattcatgg cttcctggta aacagaactg
3601 cctccgacta tccaaaccat gtctacttta cttgccaatt ccggttgttc aataagtctt
3661 aaggcatcat ccaaactttt ggcaagaaaa tgagctcctc gtggtggttc tttgagttct
3721 ctactgagaa ctatattaat tctgtccttt aaaggtcgat tcttctcagg aatggagaac
3781 caggttttcc tacccataat caccagattc tgtttacctt ccactgaaga ggttgtggtc
3841 attctttgga agtacttgaa ctcgttcctg agcggaggcc agggtaggtc tccgttcttg
3901 ccaatcccca tattttggga cacggcgacg atgcagttca atggtcgaac catgatggca
3961 gcggggataa aatcctacca gccttcacgc taggattgcc gtcaagtttg gcgcgaaatc
4021 gcagccctga gctgtgtacc acttnnnccc cccccccccc ccctgcagtt ggacctggga
4081 gtggacacct gtggagagaa aggcaaagtg gatgtcattg tcactcaagt gtatggccag
4141 atctcaagcc tgccacacct caagcttgac aacaaaaaga ttgtcttttc tgaccagatg
4201 gacgcggcca ccctcaaagg catcaccgcg ggccaggtga atatcaaatc ctcctcgttt
4261 ttggaaactg acaatcttag cgcagaagtc atgcccgctt ttgagaggga gtactcaccc
4321 caacagctgg ccctcgcaga cagcgatgcg gaagagagtg aggacgaacg cgcccccacc
4381 cccttttata gccccccttc aggaacaccc ggtcacgtgg cctacaccta taaaccaatc
4441 accttccttg atgccgaaga gggcgacatg tgtcttcaca ccctggagcg agtggacccc
4501 ctagtggaca acgaccgcta cccctcccac ttagcctcct tcgtgctggc ctggacgcga
4561 gccttcgtct cagagtggtc cgagtttcta tacgaggagg accgcggaac accgctcgag
4621 gacaggcctc tcaagtctgt atacggggac acggacagcc ttttcgtcac cgagcgtgga
4681 caccggctca tggaaaccag aggtaagaaa cgcatcaaaa agcatggggg aaacctggtt
4741 tttgaccccg aacggccaga gctcacctgg ctcgtggaat gcgagaccgt ctgcggggcc
4801 tgcggcgcgg atgcctactc cccggaatcg gtatttctcg cgcccaagct ctacgccctt
4861 aaaagtctgc actgcccctc gtgcggcgcc tcctccaagg gcaagctgcg cgccaagggc
4921 cacgccgcgg aggggctgga ctatgacacc atggtcaaat gctacctggc cgacgcgcag
4981 ggcgaagacc ggcagcgctt cagcaccagc aggaccagcc tcaagcgcac cctggccagc
5041 gcgcagcccg gagcgcaccc cttcaccgtg acccagacta cgctgacgag gaccctgcgc
5101 cnnngngaat tcnctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
5161 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
5221 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
5281 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
5341 ag
//