LOCUS LT726851 5770 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-E-hbeclin-1-C, complete sequence. ACCESSION LT726851 VERSION LT726851.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-E-hbeclin-1-C ORGANISM Mammalian expression vector pCAGGS-E-hbeclin-1-C other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5770) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5770 /organism="Mammalian expression vector pCAGGS-E-hbeclin-1-C" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 7286." /db_xref="taxon:1944773" misc_feature 16..54 /note="E-tag" CDS 64..972 /transl_table=11 /note="hBECN1-C fragment" /protein_id="SJL86674.1" /translation="MTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEE RLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSV ENQMRYAQTQLDKLKKTNVFNATFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWG QTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLYCSGGLRFFWDNKFD HAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQ WTKALKFMLTNLKWGLAWVSSQFYNK" regulatory 1060..1151 /note="3' UTR of rabbit beta-globin, incl. polyA" /regulatory_class="terminator" misc_feature 1152..1508 /note="3' flanking region of rabbit beta-globin" regulatory complement(1586..1668) /note="lac promoter" /regulatory_class="promoter" rep_origin 1810..1885 /note="SV40 ori" regulatory complement(1916..1990) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 1916..1990 /note="SV40 polyA early" /regulatory_class="terminator" rep_origin complement(2105..2506) /note="pMB1 ori" CDS complement(3047..3907) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL86675.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 4043..4420 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 4426..5767 /note="AG promoter" /regulatory_class="promoter" misc_feature 4795..5719 /note="intron AG promoter" BASE COUNT 1278 a 1468 c 1615 g 1409 t ORIGIN 1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 61 gcaatgactc agctcaacgt cactgaaaat gagtgtcaga actacaaacg ctgtttggag 121 atcttagagc aaatgaatga ggatgacagt gaacagttac agatggagct aaaggagctg 181 gcactagagg aggagaggct gatccaggag ctggaagacg tggaaaagaa ccgcaagata 241 gtggcagaaa atctcgagaa ggtccaggct gaggctgaga gactggatca ggaggaagct 301 cagtatcaga gagaatacag tgaatttaaa cgacagcagc tggagctgga tgatgagctg 361 aagagtgttg aaaaccagat gcgttatgcc cagacgcagc tggataagct gaagaaaacc 421 aacgtcttta atgcaacctt ccacatctgg cacagtggac agtttggcac aatcaataac 481 ttcaggctgg gtcgcctgcc cagtgttccc gtggaatgga atgagattaa tgctgcttgg 541 ggccagactg tgttgctgct ccatgctctg gccaataaga tgggtctgaa atttcagaga 601 taccgacttg ttccttacgg aaaccattca tatctggagt ctctgacaga caaatctaag 661 gagctgccgt tatactgttc tggggggttg cggtttttct gggacaacaa gtttgaccat 721 gcaatggtgg ctttcctgga ctgtgtgcag cagttcaaag aagaggttga gaaaggcgag 781 actcgttttt gtcttcccta caggatggat gtggagaaag gcaagattga agacacagga 841 ggcagtggcg gctcctattc catcaaaacc cagtttaact ctgaggagca gtggacaaaa 901 gctctcaagt tcatgctgac gaatcttaag tggggtcttg cttgggtgtc ctcacaattt 961 tataacaaat gagtcgagga attcactcct caggtgcagg ctgcctatca gaaggtggtg 1021 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa 1081 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt 1141 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg 1201 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc 1261 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag 1321 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt 1381 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta 1441 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta 1501 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt 1561 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 1621 agcctgggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 1681 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag 1741 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 1801 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 1861 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt 1921 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 1981 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 2041 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 2101 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 2161 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag 2221 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 2281 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 2341 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 2401 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 2461 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 2521 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 2581 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 2641 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 2701 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 2761 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 2821 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 2881 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 2941 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 3001 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 3061 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 3121 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 3181 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 3241 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 3301 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 3361 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 3421 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 3481 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 3541 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 3601 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 3661 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 3721 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 3781 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 3841 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 3901 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 3961 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 4021 cccgaaaagt gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt 4081 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 4141 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 4201 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 4261 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 4321 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 4381 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg 4441 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt 4501 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg 4561 gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag 4621 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 4681 aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc 4741 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc 4801 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt 4861 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg 4921 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg 4981 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg 5041 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac 5101 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc 5161 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt 5221 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag 5281 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg 5341 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt 5401 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa 5461 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg 5521 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc 5581 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 5641 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc 5701 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt 5761 tggcaaagaa //