LOCUS LT726849 7354 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian vector pdcDNA-Myc, complete sequence.
ACCESSION LT726849
VERSION LT726849.1
KEYWORDS .
SOURCE Mammalian vector pdcDNA-Myc
ORGANISM Mammalian vector pdcDNA-Myc
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7354)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7354
/organism="Mammalian vector pdcDNA-Myc"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 7212."
/db_xref="taxon:1945173"
regulatory 235..818
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
regulatory 863..879
/note="T7 promoter"
/regulatory_class="promoter"
regulatory 896..912
/note="SP6 promoter"
/regulatory_class="promoter"
misc_feature 964..993
/note="Myc epitope"
misc_feature 1003..1032
/note="Myc epitope"
misc_feature 1042..1071
/note="Myc epitope"
misc_feature 1081..1110
/note="Myc epitope"
misc_feature 1120..1149
/note="Myc epitope"
misc_feature 1183..1212
/note="Myc epitope"
regulatory complement(1240..1364)
/note="attR1"
/regulatory_class="attenuator"
regulatory 1372..1424
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 1473..2132
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL86669.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 2474..2779
/transl_table=11
/note="ccdB"
/protein_id="SJL86670.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 2820..2944
/note="attR2"
/regulatory_class="attenuator"
regulatory complement(2981..2997)
/note="SP6 promoter"
/regulatory_class="promoter"
regulatory 3023..3247
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 3293..3721
/note="f1 ori"
regulatory 3726..3935
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3892..3967
/note="SV40 ori"
CDS 4059..4853
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL86671.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 5027..5101
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5027..5101)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(5240..5323)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(5415..5817)
/note="pMB1 ori"
CDS complement(6358..7218)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86672.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1818 a 1780 c 1835 g 1921 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgattta
901 ggtgacacta tagaatacaa gctacttgtt ctttttgcag gatcccatcg atttaaagct
961 atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct
1021 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
1081 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
1141 gaggacttga atgaaatgga gagcttgggc gacctcacca tggagcaaaa gctcatttct
1201 gaagaggact tgaatttaga gatctcgaat tcaaggatca caagtttgta caaaaaagct
1261 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
1321 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
1381 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
1441 cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
1501 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
1561 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
1621 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
1681 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
1741 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
1801 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
1861 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
1921 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
1981 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
2041 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
2101 agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca
2161 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
2221 atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga
2281 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
2341 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
2401 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
2461 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
2521 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
2581 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
2641 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
2701 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
2761 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
2821 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
2881 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
2941 tggtgattcc cctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct
3001 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
3061 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
3121 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
3181 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
3241 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
3301 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
3361 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
3421 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta
3481 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
3541 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
3601 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
3661 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
3721 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
3781 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
3841 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
3901 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
3961 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
4021 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
4081 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
4141 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
4201 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
4261 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
4321 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
4381 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
4441 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
4501 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
4561 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
4621 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
4681 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
4741 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
4801 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
4861 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
4921 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
4981 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
5041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
5101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
5161 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
5221 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
5281 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
5341 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
5401 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
5461 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
5521 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
5581 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
5641 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
5701 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
5761 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
5821 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
5881 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
5941 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
6001 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
6061 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
6121 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
6181 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
6241 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
6301 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
6361 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
6421 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
6481 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
6541 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
6601 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
6661 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
6721 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
6781 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
6841 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
6901 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
6961 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
7021 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
7081 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
7141 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
7201 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
7261 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
7321 gcgcacattt ccccgaaaag tgccacctga cgtc
//