LOCUS LT726847 2734 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pPLc32, complete sequence.
ACCESSION LT726847
VERSION LT726847.1
KEYWORDS .
SOURCE Bacterial expression vector pPLc32
ORGANISM Bacterial expression vector pPLc32
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 2734)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..2734
/organism="Bacterial expression vector pPLc32"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 719."
/db_xref="taxon:1944701"
rep_origin complement(306..708)
/note="pMB1 ori"
CDS complement(1262..2122)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86667.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 2488..2618
/note="PL"
/regulatory_class="promoter"
BASE COUNT 703 a 693 c 679 g 659 t
ORIGIN
1 ttccggatcc ggccaagctt ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
61 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
121 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
181 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
241 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
301 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
361 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
421 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
481 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
541 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
601 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
661 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
721 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
781 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
841 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
901 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
961 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
1021 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
1081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacctctaga gggaacgaaa
1141 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
1201 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
1261 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
1321 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
1381 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
1441 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
1501 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
1561 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
1621 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
1681 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
1741 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
1801 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
1861 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
1921 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
1981 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
2041 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
2101 cacggaaatg ttgaatactc atactcttcc tttttcaata ttatgtaagc agacagtttt
2161 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
2221 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt
2281 cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc
2341 tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt
2401 caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg ccggtgatgc
2461 cggccacgat gcgtccggcg tagaggatct ctcacctacc aaacaatgcc cccctgcaaa
2521 aaataaattc atataaaaaa catacagata accatctgcg gtgataaatt atctctggcg
2581 gtgttgacat aaataccact ggcggtgata ctgagcacat cagcaggacg cactgaccac
2641 catgaaggtg acgctcttaa aattaagccc tgaagaaggg cagcattcaa agcagaaggc
2701 tttggggtgt gtgatacgaa acgaagcatt ggaa
//