LOCUS LT726846 5711 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAG-STING-SV, complete
sequence.
ACCESSION LT726846
VERSION LT726846.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAG-STING-SV
ORGANISM Mammalian expression vector pCAGGS-FLAG-STING-SV
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5711)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5711
/organism="Mammalian expression vector
pCAGGS-FLAG-STING-SV"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 6922."
/db_xref="taxon:1944864"
misc_feature 4..27
/note="FLAG epitope"
CDS 28..879
/transl_table=11
/note="hTMEM173 splice variant"
/protein_id="SJL86665.1"
/translation="MPHSSLHPSIPCPRGHGAQKAALVLLSACLVTLWGLGEPPEHTL
RYLVLHLASLQLGLLLNGVCSLAEELHHIHSRYRGSYWRTVRACLGCPLRRGALLLLS
IYFYYSLPNAVGPPFTWMLALLGLSQALNILLGLKGLAPAEISAVCEKGNFNVAHGLA
WSYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPN
IRFLDKLPQQTADRAGIKDRVYSNSIYELLENGQRNLQMTAASRCPRRFSGTCGRRKR
KRLLWAA"
regulatory 990..1081
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1082..1438
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1516..1598)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1740..1815
/note="SV40 ori"
regulatory 1846..1920
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1846..1920)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2035..2436)
/note="pMB1 ori"
CDS complement(2977..3837)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86666.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3977..4354
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4360..5701
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4729..5653
/note="intron AG promoter"
BASE COUNT 1182 a 1562 c 1590 g 1377 t
ORIGIN
1 atggactaca aggacgacga tgacaagatg ccccactcca gcctgcatcc atccatcccg
61 tgtcccaggg gtcacggggc ccagaaggca gccttggttc tgctgagtgc ctgcctggtg
121 accctttggg ggctaggaga gccaccagag cacactctcc ggtacctggt gctccaccta
181 gcctccctgc agctgggact gctgttaaac ggggtctgca gcctggctga ggagctgcac
241 cacatccact ccaggtaccg gggcagctac tggaggactg tgcgggcctg cctgggctgc
301 cccctccgcc gtggggccct gttgctgctg tccatctatt tctactactc cctcccaaat
361 gcggtcggcc cgcccttcac ttggatgctt gccctcctgg gcctctcgca ggcactgaac
421 atcctcctgg gcctcaaggg cctggcccca gctgagatct ctgcagtgtg tgaaaaaggg
481 aatttcaacg tggcccatgg gctggcatgg tcatattaca tcggatatct gcggctgatc
541 ctgccagagc tccaggcccg gattcgaact tacaatcagc attacaacaa cctgctacgg
601 ggtgcagtga gccagcggct gtatattctc ctcccattgg actgtggggt gcctgataac
661 ctgagtatgg ctgaccccaa cattcgcttc ctggataaac tgccccagca gaccgctgac
721 cgtgctggca tcaaggatcg ggtttacagc aacagcatct atgagcttct ggagaacggg
781 cagcggaacc tgcagatgac agcagcttct cgctgtccca ggaggttctc cggcacctgc
841 ggcaggagga aaaggaagag gttactgtgg gcagcttgaa gacctcagcg gtgcccagta
901 cctccacgat gtcccaagag cctgagctcc tcatcagtgg aatggaaaag cccctccctc
961 tccgcacgga tttctcttga gtcgacgaag atctttttcc ctctgccaaa aattatgggg
1021 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
1081 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
1141 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
1201 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
1261 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
1321 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
1381 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
1441 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
1501 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg
1561 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
1621 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
1681 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
1741 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
1801 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
1861 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
1921 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
1981 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
2041 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
2101 ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat
2161 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
2221 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
2281 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
2341 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
2401 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
2461 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
2521 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
2581 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
2641 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
2701 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
2761 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
2821 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
2881 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
2941 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
3001 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
3061 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
3121 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
3181 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
3241 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
3301 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
3361 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
3421 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
3481 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
3541 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
3601 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
3661 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
3721 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
3781 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
3841 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
3901 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
3961 gccacctggg tcgatcgaca ttgattattg actagttatt aatagtaatc aattacgggg
4021 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
4081 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
4141 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
4201 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
4261 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
4321 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
4381 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
4441 ttattttgtg cagcgatggg ggcggggggg gggggggggc cccccccagg cggggcgggg
4501 cggggcgagg ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa tcagagcggc
4561 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
4621 agcgcgcggc gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg ctccgccgcc
4681 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
4741 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
4801 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
4861 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
4921 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
4981 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
5041 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
5101 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
5161 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
5221 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaagg ggcgcggcgg
5281 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
5341 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
5401 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
5461 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
5521 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
5581 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
5641 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
5701 agaattccac c
//