LOCUS LT726845 5900 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAG-STING, complete sequence.
ACCESSION LT726845
VERSION LT726845.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAG-STING
ORGANISM Mammalian expression vector pCAGGS-FLAG-STING
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5900)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5900
/organism="Mammalian expression vector pCAGGS-FLAG-STING"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 6921."
/db_xref="taxon:1944863"
misc_feature 4..27
/note="FLAG epitope"
CDS 28..1167
/transl_table=11
/note="hTMEM173"
/protein_id="SJL86663.1"
/translation="MPHSSLHPSIPCPRGHGAQKAALVLLSACLVTLWGLGEPPEHTL
RYLVLHLASLQLGLLLNGVCSLAEELHHIHSRYRGSYWRTVRACLGCPLRRGALLLLS
IYFYYSLPNAVGPPFTWMLALLGLSQALNILLGLKGLAPAEISAVCEKGNFNVAHGLA
WSYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPN
IRFLDKLPQQTADRAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQ
AGFSREDRLEQAKLFCQTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLR
QEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS"
regulatory 1177..1268
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1269..1625
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1703..1785)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1927..2002
/note="SV40 ori"
regulatory 2033..2107
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2033..2107)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2222..2623)
/note="pMB1 ori"
CDS complement(3164..4024)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86664.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4164..4541
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4547..5888
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4916..5840
/note="intron AG promoter"
BASE COUNT 1222 a 1619 c 1640 g 1419 t
ORIGIN
1 atggactaca aggacgacga tgacaagatg ccccactcca gcctgcatcc atccatcccg
61 tgtcccaggg gtcacggggc ccagaaggca gccttggttc tgctgagtgc ctgcctggtg
121 accctttggg ggctaggaga gccaccagag cacactctcc ggtacctggt gctccaccta
181 gcctccctgc agctgggact gctgttaaac ggggtctgca gcctggctga ggagctgcac
241 cacatccact ccaggtaccg gggcagctac tggaggactg tgcgggcctg cctgggctgc
301 cccctccgcc gtggggccct gttgctgctg tccatctatt tctactactc cctcccaaat
361 gcggtcggcc cgcccttcac ttggatgctt gccctcctgg gcctctcgca ggcactgaac
421 atcctcctgg gcctcaaggg cctggcccca gctgagatct ctgcagtgtg tgaaaaaggg
481 aatttcaacg tggcccatgg gctggcatgg tcatattaca tcggatatct gcggctgatc
541 ctgccagagc tccaggcccg gattcgaact tacaatcagc attacaacaa cctgctacgg
601 ggtgcagtga gccagcggct gtatattctc ctcccattgg actgtggggt gcctgataac
661 ctgagtatgg ctgaccccaa cattcgcttc ctggataaac tgccccagca gaccgctgac
721 cgtgctggca tcaaggatcg ggtttacagc aacagcatct atgagcttct ggagaacggg
781 cagcgggcgg gcacctgtgt cctggagtac gccaccccct tgcagacttt gtttgccatg
841 tcacaataca gtcaagctgg ctttagccgg gaggataggc ttgagcaggc caaactcttc
901 tgccagacac ttgaggacat cctggcagat gcccctgagt ctcagaacaa ctgccgcctc
961 attgcctacc aggaacctgc agatgacagc agcttctcgc tgtcccagga ggttctccgg
1021 cacctgcggc aggaggaaaa ggaagaggtt actgtgggca gcttgaagac ctcagcggtg
1081 cccagtacct ccacgatgtc ccaagagcct gagctcctca tcagtggaat ggaaaagccc
1141 ctccctctcc gcacggattt ctcttgagtc gacgaagatc tttttccctc tgccaaaaat
1201 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
1261 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
1321 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
1381 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
1441 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
1501 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
1561 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
1621 agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
1681 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
1741 ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
1801 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc
1861 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
1921 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
1981 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt
2041 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
2101 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
2161 ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
2221 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
2281 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggat aacgcaggaa
2341 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
2401 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
2461 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
2521 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
2581 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
2641 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
2701 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
2761 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
2821 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
2881 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
2941 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
3001 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
3061 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
3121 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
3181 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
3241 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
3301 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
3361 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
3421 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
3481 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
3541 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
3601 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
3661 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
3721 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
3781 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
3841 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
3901 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
3961 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
4021 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
4081 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
4141 gaaaagtgcc acctgggtcg atcgacattg attattgact agttattaat agtaatcaat
4201 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
4261 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
4321 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
4381 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
4441 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
4501 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
4561 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
4621 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg
4681 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca
4741 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
4801 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc
4861 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
4921 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
4981 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
5041 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
5101 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
5161 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
5221 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
5281 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
5341 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
5401 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc
5461 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
5521 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
5581 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
5641 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
5701 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
5761 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
5821 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
5881 ttggcaaaga attcctcgag
//