LOCUS LT726844 5489 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAG-MDA5-CARD, complete
sequence.
ACCESSION LT726844
VERSION LT726844.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAG-MDA5-CARD
ORGANISM Mammalian expression vector pCAGGS-FLAG-MDA5-CARD
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5489)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5489
/organism="Mammalian expression vector
pCAGGS-FLAG-MDA5-CARD"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 6920."
/db_xref="taxon:1944862"
misc_feature 4..27
/note="FLAG epitope"
CDS 34..>753
/transl_table=11
/note="hIFIH1 CARD"
/protein_id="SJL86661.1"
/translation="MSNGYSTDENFRYLISCFRARVKMYIQVEPVLDYLTFLPAEVKE
QIQRTVATSGNMQAVELLLSTLEKGVWHLGWTREFVEALRRTGSPLAARYMNPELTDL
PSPSFENAHDEYLQLLNLLQPTLVDKLLVRDVLDKCMEEELLTIEDRNRIAAAENNGN
ESGVRELLKRIVQKENWFSAFLNVLRQTGNNELVQELTGSDCSESNAEIENLSQVDGP
QVEEQLLSTTVQPNLEKEVWGM"
regulatory 766..857
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 858..1214
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1292..1374)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1516..1591
/note="SV40 ori"
regulatory 1622..1696
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1622..1696)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1811..2212)
/note="pMB1 ori"
CDS complement(2753..3613)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86662.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3753..4130
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4136..5477
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4505..5429
/note="intron AG promoter"
BASE COUNT 1199 a 1426 c 1521 g 1343 t
ORIGIN
1 atggactaca aggacgacga tgacaagggt accatgtcga atgggtattc cacagacgag
61 aatttccgct atctcatctc gtgcttcagg gccagggtga aaatgtacat ccaggtggag
121 cctgtgctgg actacctgac ctttctgcct gcagaggtga aggagcagat tcagaggaca
181 gtcgccacct ccgggaacat gcaggcagtt gaactgctgc tgagcacctt ggagaaggga
241 gtctggcacc ttggttggac tcgggaattc gtggaggccc tccggagaac cggcagccct
301 ctggccgccc gctacatgaa ccctgagctc acggacttgc cctctccatc gtttgagaac
361 gctcatgatg aatatctcca actgctgaac ctccttcagc ccactctggt ggacaagctt
421 ctagttagag acgtcttgga taagtgcatg gaggaggaac tgttgacaat tgaagacaga
481 aaccggattg ctgctgcaga aaacaatgga aatgaatcag gtgtaagaga gctactaaaa
541 aggattgtgc agaaagaaaa ctggttctct gcatttctga atgttcttcg tcaaacagga
601 aacaatgaac ttgtccaaga gttaacaggc tctgattgct cagaaagcaa tgcagagatt
661 gagaatttat cacaagttga tggtcctcaa gtggaagagc aacttctttc aaccacagtt
721 cagccaaatc tggagaagga ggtctggggc atgtgactcg acgaagatct ttttccctct
781 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
841 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
901 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
961 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
1021 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
1081 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
1141 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
1201 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
1261 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
1321 gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
1381 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa
1441 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
1501 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
1561 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
1621 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
1681 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
1741 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg
1801 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
1861 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcagggata
1921 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
1981 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
2041 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
2101 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
2161 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
2221 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
2281 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
2341 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
2401 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
2461 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
2521 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
2581 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
2641 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
2701 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
2761 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
2821 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
2881 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
2941 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
3001 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
3061 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
3121 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
3181 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
3241 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
3301 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
3361 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
3421 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
3481 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
3541 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
3601 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
3661 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
3721 catttccccg aaaagtgcca cctgggtcga tcgacattga ttattgacta gttattaata
3781 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
3841 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
3901 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
3961 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
4021 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
4081 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
4141 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
4201 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc
4261 cccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc
4321 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
4381 gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt
4441 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
4501 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
4561 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt
4621 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
4681 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct
4741 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg
4801 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
4861 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc
4921 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
4981 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
5041 ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
5101 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
5161 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
5221 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
5281 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
5341 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
5401 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
5461 ctcatcattt tggcaaagaa ttcctcgag
//