LOCUS LT726843 2872 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pPLc245, complete sequence.
ACCESSION LT726843
VERSION LT726843.1
KEYWORDS .
SOURCE Bacterial expression vector pPLc245
ORGANISM Bacterial expression vector pPLc245
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 2872)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..2872
/organism="Bacterial expression vector pPLc245"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 682."
/db_xref="taxon:1944693"
misc_feature 98..130
/note="5UTR"
rep_origin complement(457..859)
/note="pMB1 ori"
CDS complement(1400..2260)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86660.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 2626..2756
/note="PL"
/regulatory_class="promoter"
BASE COUNT 744 a 733 c 706 g 689 t
ORIGIN
1 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
61 tccctcagca atcgcagcaa actccggcat ctactaatag acgccggcca ttcaaacatg
121 aggattaccc atgtcgaccc tctagaggct gcagcctcta gagccaagct tggctgcctc
181 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
241 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
301 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
361 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
421 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
481 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
541 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
601 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
661 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
721 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
781 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
841 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
901 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
961 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
1021 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
1081 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
1141 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
1201 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
1261 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
1321 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
1381 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
1441 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
1501 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
1561 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
1621 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
1681 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
1741 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
1801 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
1861 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
1921 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
1981 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
2041 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
2101 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
2161 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
2221 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
2281 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
2341 cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag
2401 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
2461 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
2521 ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga
2581 cctcagcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctct cacctaccaa
2641 acaatgcccc cctgcaaaaa ataaattcat ataaaaaaca tacagataac catctgcggt
2701 gataaattat ctctggcggt gttgacataa ataccactgg cggtgatact gagcacatca
2761 gcaggacgca ctgaccacca tgaaggtgac gctcttaaaa ttaagccctg aagaagggca
2821 gcattcaaag cagaaggctt tggggtgtgt gatacgaaac gaagcattgg aa
//