LOCUS LT726841 8342 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial two-hybrid vector pB2H-delta-omega, complete sequence.
ACCESSION LT726841
VERSION LT726841.1
KEYWORDS .
SOURCE Bacterial two-hybrid vector pB2H-delta-omega
ORGANISM Bacterial two-hybrid vector pB2H-delta-omega
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8342)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8342
/organism="Bacterial two-hybrid vector pB2H-delta-omega"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 6701."
/db_xref="taxon:1944715"
CDS complement(<78..2441)
/transl_table=11
/note="lacZ delta-omega fragment"
/protein_id="SJL86651.1"
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRA"
misc_feature complement(2503..2538)
/note="5' UTR of lacZ gene, incl. RBS"
regulatory complement(2539..2573)
/note="tac promoter"
/regulatory_class="promoter"
regulatory complement(2603..2619)
/note="SP6 promoter"
/regulatory_class="promoter"
regulatory complement(2680..2763)
/note="lac promoter"
/regulatory_class="promoter"
regulatory 3388..3435
/note="T7 terminator"
/regulatory_class="terminator"
rep_origin 3472..3927
/note="f1 ori"
CDS complement(4042..4902)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86652.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 5446..5848
/note="pMB1 ori"
CDS complement(6094..6285)
/transl_table=11
/note="rop"
/protein_id="SJL86653.1"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
ADELYRSCLARFGDDGENL"
CDS complement(6857..7939)
/transl_table=11
/note="lacI"
/protein_id="SJL86654.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
regulatory complement(7940..8017)
/note="lacI promoter"
/regulatory_class="promoter"
regulatory 8326..8342
/note="T7 promoter"
/regulatory_class="promoter"
BASE COUNT 1903 a 2253 c 2198 g 1988 t
ORIGIN
1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
61 gagatatacc atggttatgc acgggtgaac tgatcgcgca gcggcgtcag cagttgtttt
121 ttatcgccaa tccacatctg tgaaagaaag cctgactggc ggttaaattg ccaacgctta
181 ttacccagct cgatgcaaaa atccatttcg ctggtggtca gatgcgggat ggcgtgggac
241 gcggcgggga gcgtcacact gaggttttcc gccagacgcc actgctgcca ggcgctgatg
301 tgcccggctt ctgaccatgc ggtcgcgttc ggttgcacta cgcgtactgt gagccagagt
361 tgcccggcgc tctccggctg cggtagttca ggcagttcaa tcaactgttt accttgtgga
421 gcgacatcca gaggcacttc accgcttgcc agcggcttac catccagcgc caccatccag
481 tgcaggagct cgttatcgct atgacggaac aggtattcgc tggtcacttc gatggtttgc
541 ccggataaac ggaactggaa aaactgctgc tggtgttttg cttccgtcag cgctggatgc
601 ggcgtgcggt cggcaaagac cagaccgttc atacagaact ggcgatcgtt cggcgtatcg
661 ccaaaatcac cgccgtaagc cgaccacggg ttgccgtttt catcatattt aatcagcgac
721 tgatccaccc agtcccagac gaagccgccc tgtaaacggg gatactgacg aaacgcctgc
781 cagtatttag cgaaaccgcc aagactgtta cccatcgcgt gggcgtattc gcaaaggatc
841 agcgggcgcg tctctccagg tagcgaaagc cattttttga tggaccattt cggcacagcc
901 gggaagggct ggtcttcatc cacgcgcgcg tacatcgggc aaataatatc ggtggccgtg
961 gtgtcggctc cgccgccttc atactgcacc gggcgggaag gatcgacaga tttgatccag
1021 cgatacagcg cgtcgtgatt agcgccgtgg cctgattcat tccccagcga ccagatgatc
1081 acactcgggt gattacgatc gcgctgcacc attcgcgtta cgcgttcgct catcgccggt
1141 agccagcgcg gatcatcggt cagacgattc attggcacca tgccgtgggt ttcaatattg
1201 gcttcatcca ccacatacag gccgtagcgg tcgcacagcg tgtaccacag cggatggttc
1261 ggataatgcg aacagcgcac ggcgttaaag ttgttctgct tcatcagcag gatatcctgc
1321 accatcgtct gctcatccat gacctgacca tgcagaggat gatgctcgtg acggttaacg
1381 cctcgaatca gcaacggctt gccgttcagc agcagcagac cattttcaat ccgcacctcg
1441 cggaaaccga catcgcaggc ttctgcttca atcagcgtgc cgtcggcggt gtgcagttca
1501 accaccgcac gatagagatt cgggatttcg gcgctccaca gtttcgggtt ttcgacgttc
1561 agacgtagtg tgacgcgatc ggcataacca ccacgctcat cgataatttc accgccgaaa
1621 ggcgcggtgc cgctggcgac ctgcgtttca ccctgccata aagaaactgt tacccgtagg
1681 tagtcacgca actcgccgca catctgaact tcagcctcca gtacagcgcg gctgaaatca
1741 tcattaaagc gagtggcaac atggaaatcg ctgatttgtg tagtcggttt atgcagcaac
1801 gagacgtcac ggaaaatgcc gctcatccgc cacatatcct gatcttccag ataactgccg
1861 tcactccaac gcagcaccat caccgcgagg cggttttctc cggcgcgtaa aaatgcgctc
1921 aggtcaaatt cagacggcaa acgactgtcc tggccgtaac cgacccagcg cccgttgcac
1981 cacagatgaa acgccgagtt aacgccatca aaaataattc gcgtctggcc ttcctgtagc
2041 cagctttcat caacattaaa tgtgagcgag taacaacccg tcggattctc cgtgggaaca
2101 aacggcggat tgaccgtaat gggataggtt acgttggtgt agatgggcgc atcgtaaccg
2161 tgcatctgcc agtttgaggg gacgacgaca gtatcggcct caggaagatc gcactccagc
2221 cagctttccg gcaccgcttc tggtgccgga aaccaggcaa agcgccattc gccattcagg
2281 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
2341 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
2401 cgttgtaaaa cgacggccag tgaatccgta atcatggtca tacccccgga tccgaattcg
2461 agctcggcgc gcctgcagcg gccgcatgca tatggatcga tcctgtttcc tgtgtgaaat
2521 tgttatccgc tcacaattcc acacattata cgagccgatg attaattgtc aacaggggga
2581 tggggagtaa gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
2641 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
2701 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
2761 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
2821 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
2881 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
2941 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgaattaatt ctcatgtttg
3001 acagcttatc atcggataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt
3061 ctcatgtttg acagcttatc ttcgattagc tttaatgcgg tagtttatca cagttaaatt
3121 gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca
3181 ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc
3241 gggatatcgg ccggccacgc gatcgctgac gtcggtaccc tcgagtctgg taaagaaacc
3301 gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaacc
3361 taggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
3421 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
3481 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
3541 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
3601 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
3661 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
3721 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
3781 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
3841 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
3901 ttaacaaaat attaacgttt acaatttctg gcggcacgat ggcatgagat tatcaaaaag
3961 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
4021 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
4081 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
4141 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
4201 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
4261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
4321 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
4381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
4441 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
4501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
4561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
4621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
4681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
4741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
4801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
4861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaatc
4921 atgattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
4981 agaaaaataa acaaataggt catgaccaaa atcccttaac gtgagttttc gttccactga
5041 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
5101 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
5161 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
5221 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
5281 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
5341 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
5401 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
5461 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
5521 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
5581 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
5641 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
5701 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
5761 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
5821 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
5881 tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat
5941 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
6001 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
6061 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
6121 acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc
6181 tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct
6241 gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta
6301 agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat
6361 acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc
6421 ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa
6481 tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat
6541 aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac
6601 cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc
6661 gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct
6721 caacgacagg agcacgatca tgctagtcat gccccgcgcc caccggaagg agctgactgg
6781 gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac
6841 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
6901 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt
6961 ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga
7021 gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg
7081 ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt
7141 ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat
7201 cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt
7261 gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc
7321 gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc
7381 ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg
7441 taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa
7501 ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg
7561 gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac
7621 aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg
7681 cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg
7741 caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt
7801 aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg
7861 cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt
7921 ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg
7981 ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta
8041 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
8101 gcaaggaatg gtgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
8161 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
8221 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
8281 tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta
8341 ta
//