LOCUS LT726840 7549 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial two-hybrid vector pB2H-delta-alfa, complete sequence.
ACCESSION LT726840
VERSION LT726840.1
KEYWORDS .
SOURCE Bacterial two-hybrid vector pB2H-delta-alfa
ORGANISM Bacterial two-hybrid vector pB2H-delta-alfa
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7549)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7549
/organism="Bacterial two-hybrid vector pB2H-delta-alfa"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 6700."
/db_xref="taxon:1944714"
CDS complement(75..3056)
/transl_table=11
/note="lacZ delta-alfa fragment"
/protein_id="SJL86648.1"
/translation="MTMITDSLAVVARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWL
ECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDES
WLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLR
WSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCG
ELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIP
NLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHG
QVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVP
MNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDP
SRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEY
AHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTP
NDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHW
MVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAG
HISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWI
GDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTAD
TLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNC
QLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTREL
NYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPS
VSAEFQLSAGRYHYQLVWCQK"
misc_feature complement(3118..3153)
/note="5' UTR of lacZ, incl. RBS"
regulatory complement(3154..3188)
/note="tac promoter"
/regulatory_class="promoter"
regulatory complement(3218..3234)
/note="SP6 promoter"
/regulatory_class="promoter"
regulatory complement(3295..3378)
/note="lac promoter"
/regulatory_class="promoter"
regulatory 4003..4050
/note="T7 terminator"
/regulatory_class="terminator"
CDS complement(4270..4929)
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL86649.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
rep_origin complement(5483..5981)
/note="p15A ori"
CDS complement(6327..7409)
/transl_table=11
/note="lacI"
/protein_id="SJL86650.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
regulatory complement(7410..7487)
/note="lacI promoter"
/regulatory_class="promoter"
regulatory 7533..7549
/note="T7 promoter"
/regulatory_class="promoter"
BASE COUNT 1746 a 2043 c 1954 g 1806 t
ORIGIN
1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
61 gagatatacc atggttattt ttgacaccag accaactggt aatggtagcg accggcgctc
121 agctggaatt ccgccgatac tgacgggctc caggagtcgt cgccaccaat ccccatatgg
181 aaaccgtcga tattcagcca tgtgccttct tccgcgtgca gcagatggcg atggctggtt
241 tccatcagtt gctgttgact gtagcggctg atgttgaact ggaagtcgcc gcgccactgg
301 tgtgggccat aattcaattc gcgcgtcccg cagcgcagac cgttttcgct cgggaagacg
361 tacggggtat acatgtctga caatggcaga tcccagcggt caaaacaggc ggcagtaagg
421 cggtcgggat agttttcttg cggccctaat ccgagccagt ttacccgctc tgctacctgc
481 gccagctggc agttcaggcc aatccgcgcc ggatgcggtg tatcgctcgc cacttcaaca
541 tcaacggtaa tcgccatttg accactacca tcaatccggt aggttttccg gctgataaat
601 aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa tcagcaccgc atcagcaagt
661 gtatctgccg tgcactgcaa caacgctgct tcggcctggt aatggcccgc cgccttccag
721 cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac ttacgccaat gtcgttatcc
781 agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca gttgtttttt atcgccaatc
841 cacatctgtg aaagaaagcc tgactggcgg ttaaattgcc aacgcttatt acccagctcg
901 atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg cgtgggacgc ggcggggagc
961 gtcacactga ggttttccgc cagacgccac tgctgccagg cgctgatgtg cccggcttct
1021 gaccatgcgg tcgcgttcgg ttgcactacg cgtactgtga gccagagttg cccggcgctc
1081 tccggctgcg gtagttcagg cagttcaatc aactgtttac cttgtggagc gacatccaga
1141 ggcacttcac cgcttgccag cggcttacca tccagcgcca ccatccagtg caggagctcg
1201 ttatcgctat gacggaacag gtattcgctg gtcacttcga tggtttgccc ggataaacgg
1261 aactggaaaa actgctgctg gtgttttgct tccgtcagcg ctggatgcgg cgtgcggtcg
1321 gcaaagacca gaccgttcat acagaactgg cgatcgttcg gcgtatcgcc aaaatcaccg
1381 ccgtaagccg accacgggtt gccgttttca tcatatttaa tcagcgactg atccacccag
1441 tcccagacga agccgccctg taaacgggga tactgacgaa acgcctgcca gtatttagcg
1501 aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc aaaggatcag cgggcgcgtc
1561 tctccaggta gcgaaagcca ttttttgatg gaccatttcg gcacagccgg gaagggctgg
1621 tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg tggccgtggt gtcggctccg
1681 ccgccttcat actgcaccgg gcgggaagga tcgacagatt tgatccagcg atacagcgcg
1741 tcgtgattag cgccgtggcc tgattcattc cccagcgacc agatgatcac actcgggtga
1801 ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca tcgccggtag ccagcgcgga
1861 tcatcggtca gacgattcat tggcaccatg ccgtgggttt caatattggc ttcatccacc
1921 acatacaggc cgtagcggtc gcacagcgtg taccacagcg gatggttcgg ataatgcgaa
1981 cagcgcacgg cgttaaagtt gttctgcttc atcagcagga tatcctgcac catcgtctgc
2041 tcatccatga cctgaccatg cagaggatga tgctcgtgac ggttaacgcc tcgaatcagc
2101 aacggcttgc cgttcagcag cagcagacca ttttcaatcc gcacctcgcg gaaaccgaca
2161 tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac caccgcacga
2221 tagagattcg ggatttcggc gctccacagt ttcgggtttt cgacgttcag acgtagtgtg
2281 acgcgatcgg cataaccacc acgctcatcg ataatttcac cgccgaaagg cgcggtgccg
2341 ctggcgacct gcgtttcacc ctgccataaa gaaactgtta cccgtaggta gtcacgcaac
2401 tcgccgcaca tctgaacttc agcctccagt acagcgcggc tgaaatcatc attaaagcga
2461 gtggcaacat ggaaatcgct gatttgtgta gtcggtttat gcagcaacga gacgtcacgg
2521 aaaatgccgc tcatccgcca catatcctga tcttccagat aactgccgtc actccaacgc
2581 agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag gtcaaattca
2641 gacggcaaac gactgtcctg gccgtaaccg acccagcgcc cgttgcacca cagatgaaac
2701 gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca gctttcatca
2761 acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa cggcggattg
2821 accgtaatgg gataggttac gttggtgtag atgggcgcat cgtaaccgtg catctgccag
2881 tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca gctttccggc
2941 accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct gcgcaactgt
3001 tgggaagggc gatcggtgcg ggcaacgacg gccagtgaat ccgtaatcat ggtcataccc
3061 ccggatccga attcgagctc ggcgcgcctg cagcggccgc atgcatatgg atcgatcctg
3121 tttcctgtgt gaaattgtta tccgctcaca attccacaca ttatacgagc cgatgattaa
3181 ttgtcaacag ggggatgggg agtaagcttg agtattctat agtgtcacct aaatagcttg
3241 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
3301 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
3361 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
3421 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
3481 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
3541 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgaat
3601 taattctcat gtttgacagc ttatcatcgg ataacccctt ggggcctcta aacgggtctt
3661 gaggggtttt ttgttctcat gtttgacagc ttatcttcga ttagctttaa tgcggtagtt
3721 tatcacagtt aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca
3781 tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac
3841 tgccgggcct cttgcgggat atcggccggc cacgcgatcg ctgacgtcgg taccctcgag
3901 tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc acatggactc gtctactagc
3961 gcagcttaat taacctaggc tgctgccacc gctgagcaat aactagcata accccttggg
4021 gcctctaaac gggtcttgag gggttttttg ctgaaacctc aggcatttga gaagcacacg
4081 gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt
4141 taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca
4201 tccgcttatt atcacttatt caggcgtagc accaggcgtt taagggcacc aataactgcc
4261 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
4321 tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag
4381 caccttgtcg ccttgcgtat aatatttgcc catagtgaaa acgggggcga agaagttgtc
4441 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
4501 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
4561 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
4621 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
4681 caccagctca ccgtctttca ttgccatacg gaactccgga tgagcattca tcaggcgggc
4741 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
4801 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
4861 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
4921 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
4981 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
5041 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
5101 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc
5161 tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt
5221 ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg
5281 ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt
5341 cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg
5401 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
5461 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata
5521 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
5581 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
5641 gataccaggc gtttcccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt
5701 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt
5761 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc
5821 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac
5881 cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt
5941 taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg
6001 ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg
6061 ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat
6121 cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
6181 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg
6241 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
6301 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
6361 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc
6421 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg
6481 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt
6541 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact
6601 accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc
6661 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc
6721 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga
6781 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa
6841 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg
6901 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag
6961 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg
7021 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc
7081 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc
7141 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga
7201 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg
7261 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa
7321 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct
7381 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg
7441 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg
7501 ctctccctta tgcgactcct gcattaggaa attaatacga ctcactata
//