LOCUS LT726839 10353 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pAS2-mRIP1, complete sequence.
ACCESSION LT726839
VERSION LT726839.1
KEYWORDS .
SOURCE Yeast expression vector pAS2-mRIP1
ORGANISM Yeast expression vector pAS2-mRIP1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 10353)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..10353
/organism="Yeast expression vector pAS2-mRIP1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 6680."
/db_xref="taxon:1945462"
rep_origin complement(2..982)
/note="2mu ori"
regulatory complement(1060..1164)
/note="FLP terminator"
/regulatory_class="terminator"
CDS 1884..2558
/transl_table=11
/note="TRP1"
/protein_id="SJL86643.1"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK"
rep_origin complement(2661..3116)
/note="f1 ori"
regulatory complement(3312..3586)
/note="CYC1 terminator"
/regulatory_class="terminator"
CDS complement(3855..4304)
/transl_table=11
/note="CYH2"
/protein_id="SJL86644.1"
/translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN
MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI
DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA"
regulatory 5075..5478
/note="ADH1 promoter"
/regulatory_class="promoter"
CDS 5501..>5941
/transl_table=11
/note="GALbd"
/protein_id="SJL86645.1"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
misc_feature 5957..5983
/note="HA epitope"
CDS 5987..7957
/transl_table=11
/note="mRIP1"
/protein_id="SJL86646.1"
/translation="MQPDMSLDNIKMASSDLLEKTDLDSGGFGKVSLCYHRSHGFVIL
KKVYTGPNRAEYNEVLLEEGKMMHRLRHSRVVKLLGIIIEEGNYSLVMEYMEKGNLMH
VLKTQIDVPLSLKGRIIVEAIEGMCYLHDKGVIHKDLKPENILVDRDFHIKIADLGVA
SFKTWSKLTKEKDNKQKEVSSTTKKNNGGTLYYMAPEHLNDINAKPTEKSDVYSFGIV
LWAIFAKKEPYENVICTEQFVICIKSGNRPNVEEILEYCPREIISLMERCWQAIPEDR
PTFLGIEEEFRPFYLSHFEEYVEEDVASLKKEYPDQSPVLQRMFSLQHDCVPLPPSRS
NSEQPGSLHSSQGLQMGPVEESWFSSSPEYPQDENDRSVQAKLQEEASYHAFGIFAEK
QTKPQPRQNEAYNREEERKRRVSHDPFAQQRARENIKSAGARGHSDPSTTSRGIAVQQ
LSWPATQTVWNNGLYNQHGFGTTGTGVWYPPNLSQMYSTYKTPVPETNIPGSTPTMPY
FSGPVADDLIKYTIFNSSGIQIGNHNYMDVGLNSQPPNNTCKEESTSRHQAIFDNTTS
LTDEHLNPIRENLGRQWKNCARKLGFTESQIDEIDHDYERDGLKEKVYQMLQKWLMRE
GTKGATVGKLAQALHQCCRIDLLNHLIRASQS"
regulatory 7991..8092
/note="ADH1 terminator"
/regulatory_class="terminator"
regulatory complement(8245..8328)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(8420..8822)
/note="pMB1 ori"
CDS complement(9363..10223)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86647.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2973 a 2179 c 2366 g 2835 t
ORIGIN
1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt
1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta
1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat
1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac
1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa
1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg
2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag
2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg
2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct
2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa
2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga
3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt
3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca
3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga
3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct
3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct
3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc
3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta
4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc
4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc
4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg
4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag
4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat
4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag
4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata
4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg
4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat
4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt
4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc
4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca
4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg
4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt
4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca
4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa
4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt
5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt
5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac
5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
5221 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt
5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat
5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcatgc aaccagacat
6001 gtccttggac aatattaaga tggcatccag tgacctgctg gagaagacag acctagacag
6061 cggaggcttc gggaaggtgt ccttgtgtta ccacagaagc catggatttg tcatcctgaa
6121 aaaagtatac acagggccca accgcgctga gtacaatgag gttctcttgg aagaggggaa
6181 gatgatgcac agactgagac acagtcgagt ggtgaagcta ctgggcatca tcatagaaga
6241 agggaactat tcgctggtga tggagtacat ggagaagggc aacctgatgc acgtgctaaa
6301 gacccagata gatgtcccac tttcattgaa aggaaggata atcgtggagg ccatagaagg
6361 catgtgctac ttacatgaca aaggtgtgat acacaaggac ctgaagcctg agaatatcct
6421 cgttgatcgt gactttcaca ttaagatagc cgatcttggt gtggcttcct ttaagacatg
6481 gagcaaactg actaaggaga aagacaacaa gcagaaagaa gtgagcagca ccactaagaa
6541 gaacaatggt ggtacccttt actacatggc acccgaacac ctgaatgaca tcaatgcaaa
6601 gcccacggag aagtcggacg tgtacagctt tggcattgtc ctttgggcaa tatttgcaaa
6661 aaaggagccc tatgagaatg tcatctgtac tgagcagttc gtgatctgca taaaatctgg
6721 gaacaggcca aatgtagagg aaatccttga gtactgtcca agggagatca tcagcctcat
6781 ggagcggtgc tggcaggcga tcccagaaga caggccaaca tttcttggca ttgaagaaga
6841 atttaggcct ttttacttaa gtcattttga agaatatgta gaagaggatg tggcaagttt
6901 aaagaaagag tatccagatc aaagcccagt gctgcagaga atgttttcac tgcagcatga
6961 ctgtgtaccc ttacctccga gcaggtcaaa ttcagaacaa cctggatcgc tgcacagttc
7021 ccaggggctc cagatgggtc ctgtggagga gtcctggttt tcttcctccc cagagtaccc
7081 acaggacgag aatgatcgca gtgtgcaggc taagctgcaa gaggaagcca gctatcatgc
7141 ttttggaata tttgcagaga aacagacaaa accgcagcca aggcagaatg aggcttacaa
7201 cagagaggag gaaaggaaac gaagggtctc tcatgacccc tttgcacagc agagagctcg
7261 tgagaatatt aagagtgcag gagcaagagg tcattctgat cccagcacaa cgagtcgtgg
7321 aattgcagtg caacagctgt catggccagc cacccaaaca gtttggaaca atggattgta
7381 taatcagcat ggatttggaa ctacaggtac aggagtttgg tatccgccaa atctaagcca
7441 aatgtatagt acttataaaa ctccagtgcc agagaccaac ataccgggaa gcacacccac
7501 catgccatac ttctctgggc cagtagcaga tgacctcata aaatatacta tattcaatag
7561 ttctggtatt cagattggaa accacaatta tatggatgtt ggactgaatt cacaaccacc
7621 aaacaatact tgcaaagaag agtcgacttc cagacaccaa gccatctttg ataacaccac
7681 tagtctgact gatgaacacc tgaaccctat cagggaaaac ctgggaaggc agtggaaaaa
7741 ctgtgcccgc aagctgggct tcactgagtc tcagatcgat gaaatcgacc atgactatga
7801 aagagatgga ctgaaagaga aagtttacca aatgcttcag aagtggctga tgcgggaagg
7861 caccaaaggg gccacagtgg gaaagttggc ccaggcactt caccaatgtt gcaggataga
7921 cctgctgaac cacttgattc gtgccagcca gagctaagga tccgtcgacc tgcagccaag
7981 ctaattccgg gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
8041 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttgttctt
8101 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
8161 tgaccacacc tctaccggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
8221 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
8281 gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct
8341 ttccagtcgg gaaacctgtc gtgccagctg gattaatgaa tcggccaacg cgcggggaga
8401 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
8461 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
8521 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
8581 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
8641 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
8701 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
8761 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
8821 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
8881 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
8941 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
9001 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
9061 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
9121 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
9181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
9241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
9301 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
9361 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
9421 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
9481 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
9541 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
9601 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
9661 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
9721 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
9781 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
9841 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
9901 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
9961 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
10021 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
10081 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
10141 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
10201 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
10261 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
10321 gttccgcgca catttccccg aaaagtgcca cct
//