LOCUS LT726838 4679 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pBlue-loxP-PGK-gb2-hyg-loxP, complete
sequence.
ACCESSION LT726838
VERSION LT726838.1
KEYWORDS .
SOURCE Mammalian expression vector pBlue-loxP-PGK-gb2-hyg-loxP
ORGANISM Mammalian expression vector pBlue-loxP-PGK-gb2-hyg-loxP
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4679)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4679
/organism="Mammalian expression vector
pBlue-loxP-PGK-gb2-hyg-loxP"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 6665."
/db_xref="taxon:1944751"
misc_feature 7..40
/note="loxP"
regulatory 42..552
/note="mPGK1 promoter"
/regulatory_class="promoter"
regulatory 565..630
/note="synthetic EM7 promoter"
/regulatory_class="promoter"
CDS 631..1656
/transl_table=11
/note="hygromycin resistance"
/protein_id="SJL86641.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE"
regulatory 1677..1753
/note="synthetic polyA"
/regulatory_class="terminator"
misc_feature 1754..1787
/note="loxP"
regulatory complement(1823..1839)
/note="T3 promoter"
/regulatory_class="promoter"
misc_feature complement(1865..1902)
/note="5' UTR of lacZ, incl. RBS"
regulatory complement(1903..1986)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(2078..2480)
/note="pMB1 ori"
CDS complement(3021..3881)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86642.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin complement(4012..4467)
/note="f1 ori"
regulatory 4635..4651
/note="T7 promoter"
/regulatory_class="promoter"
BASE COUNT 1091 a 1219 c 1268 g 1101 t
ORIGIN
1 ggccgcataa cttcgtatag catacattat acgaagttat attctaccgg gtaggggagg
61 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
121 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
181 cgttctttgg tggccccgtc gcgccacctt ctactcctcc cctagtcagg aagttccccc
241 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
301 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
361 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
421 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
481 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
541 gcctttcgac ctgcagcagc acgtgttgac aattaatcat cggcatagta tatcggcata
601 gtataatacg acaaggtgag gaactaaacc atgaaaaagc ctgaactcac cgcgacgtct
661 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
721 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatacgt cctgcgggta
781 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
841 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
901 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
961 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
1021 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
1081 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
1141 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
1201 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
1261 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
1321 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
1381 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
1441 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
1501 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
1561 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tacttgccga tagtggaaac
1621 cgacgcccca gcactcgtcc gagggcaaag gaataggttt ccctgccaca gtctgagagc
1681 tccctggcga attcggtacc aataaaagag ctttattttc atgatctgtg tgttggtttt
1741 tgtgtgcggc gcgataactt cgtatagcat acattatacg aagttatctc gagggggggc
1801 ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
1861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
1921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
1981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
2041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
2101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
2161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
2221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
2281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
2341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
2401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
2461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
2521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
2581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
2641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
2701 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
2761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
2821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
2881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
2941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
3001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
3061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
3121 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
3181 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
3241 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
3301 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
3361 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
3421 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
3481 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
3541 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
3601 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
3661 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
3721 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
3781 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
3841 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
3901 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
3961 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
4021 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
4081 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
4141 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
4201 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
4261 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
4321 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
4381 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
4441 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
4501 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
4561 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
4621 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctccac cgcggtggc
//