LOCUS LT726836 3912 bp DNA circular SYN 06-FEB-2017 DEFINITION Yeast expression vector pPICZBBAX, complete sequence. ACCESSION LT726836 VERSION LT726836.1 KEYWORDS . SOURCE Yeast expression vector pPICZBBAX ORGANISM Yeast expression vector pPICZBBAX other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3912) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..3912 /organism="Yeast expression vector pPICZBBAX" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 6635." /db_xref="taxon:1945491" misc_feature 17..46 /note="Myc epitope" misc_feature 62..79 /note="His-tag" regulatory 159..418 /note="AOX1 terminator" /regulatory_class="terminator" regulatory 425..830 /note="TEF1 promoter" /regulatory_class="promoter" regulatory 839..904 /note="synthetic EM7 promoter" /regulatory_class="promoter" CDS 905..1279 /transl_table=11 /note="zeocin resistance" /protein_id="SJL86637.1" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW GREFALRDPAGNCVHFVAEEQD" regulatory 1325..1595 /note="CYC1 terminator" /regulatory_class="terminator" rep_origin complement(1605..1885) /note="pMB1 ori" regulatory 2342..3107 /note="AOX1 promoter" /regulatory_class="promoter" misc_feature 3108..3272 /note="5' UTR of AOX1" CDS 3314..3892 /transl_table=11 /note="mBax" /protein_id="SJL86638.1" /translation="MDGSGEQLGSGGPTSSEQIMKTGAFLLQGFIQDRAGRMAGETPE LTLEQPPQDASTKKLSECLRRIGDELDSNMELQRMIADVDTDSPREVFFRVAADMFAD GNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLVWIQDQGGWEG LLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG" BASE COUNT 940 a 985 c 930 g 1057 t ORIGIN 1 ggccgccagc tttctagaac aaaaactcat ctcagaagag gatctgaata gcgccgtcga 61 ccatcatcat catcatcatt gagtttgtag ccttagacat gactgttcct cagttcaagt 121 tgggcactta cgagaagacc ggtcttgcta gattctaatc aagaggatgt cagaatgcca 181 tttgcctgag agatgcaggc ttcatttttg atactttttt atttgtaacc tatatagtat 241 aggatttttt ttgtcatttt gtttcttctc gtacgagctt gctcctgatc agcctatctc 301 gcagctgatg aatatcttgt ggtaggggtt tgggaaaatc attcgagttt gatgtttttc 361 ttggtatttc ccactcctct tcagagtaca gaagattaag tgagaccttc gtttgtgcgg 421 atcccccaca caccatagct tcaaaatgtt tctactcctt ttttactctt ccagattttc 481 tcggactccg cgcatcgccg taccacttca aaacacccaa gcacagcata ctaaattttc 541 cctctttctt cctctagggt gtcgttaatt acccgtacta aaggtttgga aaagaaaaaa 601 gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt atcacgtttc 661 tttttcttga aatttttttt tttagttttt ttctctttca gtgacctcca ttgatattta 721 agttaataaa cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa 781 ctttttttac ttcttgttca ttagaaagaa agcatagcaa tctaatctaa ggggcggtgt 841 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta 901 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc 961 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc 1021 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc 1081 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc 1141 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga 1201 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt 1261 ggccgaggag caggactgac acgtccgacg gcggcccacg ggtcccaggc ctcggagatc 1321 cgtccccctt ttcctttgtc gatatcatgt aattagttat gtcacgctta cattcacgcc 1381 ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc 1441 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc 1501 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga 1561 aggttttggg acgctcgaag gctttaattt gcaagctgga gaccaacatg tgagcaaaag 1621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 1681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 1741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 1801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 1861 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 1921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 1981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 2041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 2101 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 2161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 2221 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 2281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agatcagatc 2341 taacatccaa agacgaaagg ttgaatgaaa cctttttgcc atccgacatc cacaggtcca 2401 ttctcacaca taagtgccaa acgcaacagg aggggataca ctagcagcag accgttgcaa 2461 acgcaggacc tccactcctc ttctcctcaa cacccacttt tgccatcgaa aaaccagccc 2521 agttattggg cttgattgga gctcgctcat tccaattcct tctattaggc tactaacacc 2581 atgactttat tagcctgtct atcctggccc ccctggcgag gttcatgttt gtttatttcc 2641 gaatgcaaca agctccgcat tacacccgaa catcactcca gatgagggct ttctgagtgt 2701 ggggtcaaat agtttcatgt tccccaaatg gcccaaaact gacagtttaa acgctgtctt 2761 ggaacctaat atgacaaaag cgtgatctca tccaagatga actaagtttg gttcgttgaa 2821 atgctaacgg ccagttggtc aaaaagaaac ttccaaaagt cggcataccg tttgtcttgt 2881 ttggtattga ttgacgaatg ctcaaaaata atctcattaa tgcttagcgc agtctctcta 2941 tcgcttctga accccggtgc acctgtgccg aaacgcaaat ggggaaacac ccgctttttg 3001 gatgattatg cattgtctcc acattgtatg cttccaagat tctggtggga atactgctga 3061 tagcctaacg ttcatgatca aaatttaact gttctaaccc ctacttgaca gcaatatata 3121 aacagaagga agctgccctg tcttaaacct ttttttttat catcattatt agcttacttt 3181 cataattgcg actggttcca attgacaagc ttttgatttt aacgactttt aacgacaact 3241 tgagaagatc aaaaaacaac taattattcg aaacgaggaa ttcgagctcg gtacccgggg 3301 atcctctaga gtcatggacg ggtccgggga gcagcttggg agcggcgggc ccaccagctc 3361 tgaacagatc atgaagacag gggccttttt gctacagggt ttcatccagg atcgagcagg 3421 gaggatggct ggggagacac ctgagctgac cttggagcag ccgccccagg atgcgtccac 3481 caagaagctg agcgagtgtc tccggcgaat tggagatgaa ctggatagca atatggagct 3541 gcagaggatg attgctgacg tggacacgga ctccccccga gaggtcttct tccgggtggc 3601 agctgacatg tttgctgatg gcaacttcaa ctggggccgc gtggttgccc tcttctactt 3661 tgctagcaaa ctggtgctca aggccctgtg cactaaagtg cccgagctga tcagaaccat 3721 catgggctgg acactggact tcctccgtga gcggctgctt gtctggatcc aagaccaggg 3781 tggctgggaa ggcctcctct cctacttcgg gacccccaca tggcagacag tgaccatctt 3841 tgtggctgga gtcctcaccg cctcgctcac catctggaag aagatgggct gagacctgca 3901 ggcatgcaag ct //