LOCUS LT726834 7736 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-CYLD, complete sequence.
ACCESSION LT726834
VERSION LT726834.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-CYLD
ORGANISM Mammalian expression vector pCAGGS-E-CYLD
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7736)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7736
/organism="Mammalian expression vector pCAGGS-E-CYLD"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 6613."
/db_xref="taxon:1944762"
misc_feature <11..49
/note="E-tag"
CDS 59..2929
/transl_table=11
/note="hCYLD"
/protein_id="SJL86632.1"
/translation="MSSGLWSQEKVTSPYWEERIFYLLLQECSVTDKQTQKLLKVPKG
SIGQYIQDRSVGHSRIPSAKGKKNQIGLKILEQPHAVLFVDEKDVVEINEKFTELLLA
ITNCEERFSLFKNRNRLSKGLQIDVGCPVKVQLRSGEEKFPGVVRFRGPLLAERTVSG
IFFGVELLEEGRGQGFTDGVYQGKQLFQCDEDCGVFVALDKLELIEDDDTALESDYAG
PGDTMQVELPPLEINSRVSLKVGETIESGTVIFCDVLPGKESLGYFVGVDMDNPIGNW
DGRFDGVQLCSFACVESTILLHINDIIPALSESVTQERRPPKLAFMSRGVGDKGSSSH
NKPKATGSTSDPGNRNRSELFYTLNGSSVDSQPQSKSKNTWYIDEVAEDPAKSLTEIS
TDFDRSSPPLQPPPVNSLTTENRFHSLPFSLTKMPNTNGSIGHSPLSLSAQSVMEELN
TAPVQESPPLAMPPGNSHGLEVGSLAEVKENPPFYGVIRWIGQPPGLNEVLAGLELED
ECAGCTDGTFRGTRYFTCALKKALFVKLKSCRPDSRFASLQPVSNQIERCNSLAFGGY
LSEVVEENTPPKMEKEGLEIMIGKKKGIQGHYNSCYLDSTLFCLFAFSSVLDTVLLRP
KEKNDVEYYSETQELLRTEIVNPLRIYGYVCATKIMKLRKILEKVEAASGFTSEEKDP
EEFLNILFHHILRVEPLLKIRSAGQKVQDCYFYQIFMEKNEKVGVPTIQQLLEWSFIN
SNLKFAEAPSCLIIQMPRFGKDFKLFKKIFPSLELNITDLLEDTPRQCRICGGLAMYE
CRECYDDPDISAGKIKQFCKTCNTQVHLHPKRLNHKYNPVSLPKDLPDWDWRHGCIPC
QNMELFAVLCIETSHYVAFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVGE
YLKMSLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK"
regulatory 3026..3117
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 3118..3474
/note="3' flanking region of rabbit beta-globin"
regulatory complement(3552..3634)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 3776..3851
/note="SV40 ori"
regulatory 3882..3956
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3882..3956)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(4071..4472)
/note="pMB1 ori"
CDS complement(5013..5873)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86633.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 6009..6386
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 6392..7733
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 6761..7685
/note="intron AG promoter"
BASE COUNT 1891 a 1856 c 2023 g 1966 t
ORIGIN
1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgcaat
61 gagttcaggc ttatggagcc aagaaaaagt cacttcaccc tactgggaag agcggatttt
121 ttacttgctt cttcaagaat gcagcgttac agacaaacaa acacaaaagc tccttaaagt
181 accgaaggga agtataggac agtatattca agatcgttct gtggggcatt caaggattcc
241 ttctgcaaaa ggcaagaaaa atcagattgg attaaaaatt ctagagcaac ctcatgcagt
301 tctctttgtt gatgaaaagg atgttgtaga gataaatgaa aagttcacag agttactttt
361 ggcaattacc aattgtgagg agaggttcag cctgtttaaa aacagaaaca gactaagtaa
421 aggcctccaa atagacgtgg gctgtcctgt gaaagtacag ctgagatctg gggaagaaaa
481 atttcctgga gttgtacgct tcagaggacc cctgttagca gagaggacag tctccggaat
541 attctttgga gttgaattgc tggaagaagg tcgtggtcaa ggtttcactg acggggtgta
601 ccaagggaaa cagctttttc agtgtgatga agattgtggc gtgtttgttg cattggacaa
661 gctagaactc atagaagatg atgacactgc attggaaagt gattacgcag gtcctgggga
721 cacaatgcag gtcgaacttc ctcctttgga aataaactcc agagtttctt tgaaggttgg
781 agaaacaata gaatctggaa cagttatatt ctgtgatgtt ttgccaggaa aagaaagctt
841 aggatatttt gttggtgtgg acatggataa ccctattggc aactgggatg gaagatttga
901 tggagtgcag ctttgtagtt ttgcgtgtgt tgaaagtaca attctattgc acatcaatga
961 tatcatccca gctttatcag agagtgtgac gcaggaaagg aggcctccca aacttgcctt
1021 tatgtcaaga ggtgttgggg acaaaggttc atccagtcat aataaaccaa aggctacagg
1081 atctacctca gaccctggaa atagaaacag atctgaatta ttttatacct taaatgggtc
1141 ttctgttgac tcacaaccac aatccaaatc aaaaaataca tggtacattg atgaagttgc
1201 agaagaccct gcaaaatctc ttacagagat atctacagac tttgaccgtt cttcaccacc
1261 actccagcct cctcctgtga actcactgac caccgagaac agattccact ctttaccatt
1321 cagtctcacc aagatgccca ataccaatgg aagtattggc cacagtccac tttctctgtc
1381 agcccagtct gtaatggaag agctaaacac tgcacccgtc caagagagtc cacccttggc
1441 catgcctcct gggaactcac atggtctaga agtgggctca ttggctgaag ttaaggagaa
1501 ccctcctttc tatggggtaa tccgttggat cggtcagcca ccaggactga atgaagtgct
1561 cgctggactg gaactggaag atgagtgtgc aggctgtacg gatggaacct tcagaggcac
1621 tcggtatttc acctgtgccc tgaagaaggc gctgtttgtg aaactgaaga gctgcaggcc
1681 tgactctagg tttgcatcat tgcagccggt ttccaatcag attgagcgct gtaactcttt
1741 agcatttgga ggctacttaa gtgaagtagt agaagaaaat actccaccaa aaatggaaaa
1801 agaaggcttg gagataatga ttgggaagaa gaaaggcatc cagggtcatt acaattcttg
1861 ttacttagac tcaaccttat tctgcttatt tgcttttagt tctgttctgg acactgtgtt
1921 acttagaccc aaagaaaaga acgatgtaga atattatagt gaaacccaag agctactgag
1981 gacagaaatt gttaatcctc tgagaatata tggatatgtg tgtgccacaa aaattatgaa
2041 actgaggaaa atacttgaaa aggtggaggc tgcatcagga tttacctctg aagaaaaaga
2101 tcctgaggaa ttcttgaata ttctgtttca tcatatttta agggtagaac ctttgctaaa
2161 aataagatca gcaggtcaaa aggtacaaga ttgttacttc tatcaaattt ttatggaaaa
2221 aaatgagaaa gttggcgttc ccacaattca gcagttgtta gaatggtctt ttatcaacag
2281 taacctgaaa tttgcagagg caccatcatg tctgattatt cagatgcctc gatttggaaa
2341 agactttaaa ctatttaaaa aaatttttcc ttctctggaa ttaaatataa cagatttact
2401 tgaagacact cccagacagt gccggatatg tggagggctt gcaatgtatg agtgtagaga
2461 atgctacgac gatccggaca tctcagctgg aaaaatcaag cagttttgta aaacctgcaa
2521 cactcaagtc caccttcatc cgaagaggct gaatcataaa tataacccag tgtcacttcc
2581 caaagactta cccgactggg actggagaca cggctgcatc ccttgccaga atatggagtt
2641 atttgctgtt ctctgcatag aaacaagcca ctatgttgct tttgtgaagt atgggaagga
2701 cgattctgcc tggctcttct ttgacagcat ggccgatcgg gatggtggtc agaatggctt
2761 caacattcct caagtcaccc catgcccaga agtaggagag tacttgaaga tgtctctgga
2821 agacctgcat tccttggact ccaggagaat ccaaggctgt gcacgaagac tgctttgtga
2881 tgcatatatg tgcatgtacc agagtccaac aatgagtttg tacaaataag acctcgaggt
2941 cgaggaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
3001 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
3061 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
3121 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
3181 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
3241 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
3301 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
3361 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
3421 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
3481 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
3541 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tgggtgccta
3601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
3661 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact
3721 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
3781 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
3841 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt
3901 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
3961 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
4021 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
4081 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
4141 ctcaaaggcg gtaatacggt tatccacaga atcagggata acgcaggaaa gaacatgtga
4201 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
4261 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
4321 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
4381 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
4441 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
4501 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
4561 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
4621 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
4681 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
4741 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
4801 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
4861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
4921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
4981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
5041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
5101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
5161 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
5221 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
5281 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
5341 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
5401 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
5461 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
5521 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
5581 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
5641 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
5701 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
5761 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
5821 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
5881 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
5941 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
6001 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
6061 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
6121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
6181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
6241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
6301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
6361 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
6421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
6481 tgcagcgatg ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga
6541 ggggcggggc ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg
6601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
6661 gcgggcggga gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg
6721 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
6781 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
6841 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
6901 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
6961 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
7021 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
7081 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
7141 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
7201 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
7261 cggggcgggg ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga
7321 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
7381 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc
7441 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg
7501 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg
7561 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct
7621 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
7681 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaa
//