LOCUS LT726833 5722 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-RIG-I-CARD, complete sequence.
ACCESSION LT726833
VERSION LT726833.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-RIG-I-CARD
ORGANISM Mammalian expression vector pCAGGS-E-RIG-I-CARD
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5722)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5722
/organism="Mammalian expression vector
pCAGGS-E-RIG-I-CARD"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 6517."
/db_xref="taxon:1944814"
misc_feature 16..54
/note="E-tag"
CDS 64..>915
/transl_table=11
/note="hDDX58 CARD"
/protein_id="SJL86630.1"
/translation="MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAE
KNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEE
YRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLL
RSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPE
CQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLL
ICEHHLKK"
regulatory 1012..1103
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1104..1460
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1538..1620)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1762..1837
/note="SV40 ori"
regulatory 1868..1942
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1868..1942)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2057..2458)
/note="pMB1 ori"
CDS complement(2999..3859)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86631.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3995..4372
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4378..5719
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4747..5671
/note="intron AG promoter"
BASE COUNT 1295 a 1454 c 1551 g 1422 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcaatgacca ccgagcagcg acgcagcctg caagccttcc aggattatat ccggaagacc
121 ctggacccta cctacatcct gagctacatg gccccctggt ttagggagga agaggtgcag
181 tatattcagg ctgagaaaaa caacaagggc ccaatggagg ctgccacact ttttctcaag
241 ttcctgttgg agctccagga ggaaggctgg ttccgtggct ttttggatgc cctagaccat
301 gcaggttatt ctggacttta tgaagccatt gaaagttggg atttcaaaaa aattgaaaag
361 ttggaggagt atagattact tttaaaacgt ttacaaccag aatttaaaac cagaattatc
421 ccaaccgata tcatttctga tctgtctgaa tgtttaatta atcaggaatg tgaagaaatt
481 ctacagattt gctctactaa ggggatgatg gcaggtgcag agaaattggt ggaatgcctt
541 ctcagatcag acaaggaaaa ctggcccaaa actttgaaac ttgctttgga gaaagaaagg
601 aacaagttca gtgaactgtg gattgtagag aaaggtataa aagatgttga aacagaagat
661 cttgaggata agatggaaac ttctgacata cagattttct accaagaaga tccagaatgc
721 cagaatctta gtgagaattc atgtccacct tcagaagtgt ctgatacaaa cttgtacagc
781 ccatttaaac caagaaatta ccaattagag cttgctttgc ctgctatgaa aggaaaaaac
841 acaataatat gtgctcctac aggttgtgga aaaacctttg tttcactgct tatatgtgaa
901 catcatctta aaaaatgact cgaggtcgag gaattcactc ctcaggtgca ggctgcctat
961 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
1021 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
1081 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
1141 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
1201 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
1261 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
1321 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
1381 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
1441 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
1501 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
1561 cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
1621 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt
1681 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
1741 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
1801 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
1861 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
1921 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
1981 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc
2041 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
2101 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
2161 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
2221 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
2281 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
2341 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
2401 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
2461 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
2521 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
2581 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
2641 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
2701 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
2761 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
2821 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
2881 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
2941 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
3001 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
3061 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
3121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
3181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
3241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
3301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
3361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
3421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
3481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
3541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
3601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
3661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
3721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
3781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
3841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
3901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
3961 cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa
4021 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
4081 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
4141 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
4201 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
4261 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
4321 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
4381 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
4441 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc
4501 cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg
4561 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
4621 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc
4681 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
4741 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
4801 tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct
4861 ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
4921 tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
4981 ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc
5041 tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg
5101 ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc
5161 ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg
5221 gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg
5281 ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
5341 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
5401 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
5461 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
5521 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
5581 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
5641 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
5701 gtctcatcat tttggcaaag aa
//