LOCUS LT726829 7258 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-E-hUNR, complete sequence. ACCESSION LT726829 VERSION LT726829.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-E-hUNR ORGANISM Mammalian expression vector pCAGGS-E-hUNR other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7258) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7258 /organism="Mammalian expression vector pCAGGS-E-hUNR" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 6421." /db_xref="taxon:1944786" misc_feature 11..49 /note="E-tag" CDS 59..2455 /transl_table=11 /note="hUNR" /protein_id="SJL86617.1" /translation="MSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSE RQARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEILPEERMNG QVVCAVPHNLESKSPAAPGQSPTGSVCYERNGEVFYLTYTPEDVEGNVQLETGDKINF VIDNNKHTGAVSARNIMLLKKKQARCQGVVCAMKEAFGFIERGDVVKEIFFHYSEFKG DLETLQPGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPS KNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLLEGDHVRFNISTDRRDKLERATNI EVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDVRMFFHFSEILDGNQLHIADEVE FTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTTSPNKGK EKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVEFSISDKQRPGQQVATCVR LLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGDMVEYSL SKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRSVDPTQTEYQGMIEIVEE GDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTMAYNITPLRRATVECVK DQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQRTGKCSACNVWRVCE GPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMGFGAERKIRQAGVID " regulatory 2543..2634 /note="beta-globin polyA" /regulatory_class="terminator" misc_feature 2635..2991 /note="3' FR of beta-globin" regulatory complement(3069..3151) /note="lac promoter" /regulatory_class="promoter" rep_origin 3293..3368 /note="SV40 ori" regulatory complement(3399..3473) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 3399..3473 /note="SV40 polyA early" /regulatory_class="terminator" rep_origin complement(3588..3989) /note="pMB1 ori" CDS complement(4530..5390) /transl_table=11 /note="ampicillin" /protein_id="SJL86618.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 5526..5903 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 5909..7250 /note="AG promoter" /regulatory_class="promoter" misc_feature 6278..7202 /note="intron AG promoter" BASE COUNT 1737 a 1747 c 1949 g 1825 t ORIGIN 1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgctat 61 gagctttgat ccaaaccttc tccacaacaa tggacataat gggtacccta atggtacttc 121 agcagcactg cgtgaaactg gggttattga aaaactgtta acctcttacg gatttattca 181 gtgttcagaa cgtcaagcta gacttttctt ccactgttca cagtataatg gcaacctgca 241 agacttaaaa gtaggagatg atgttgaatt tgaagtatca tcggaccgac ggactgggaa 301 acccattgct gttaaactgg tgaagataaa acaagaaatc ctccctgaag aacgaatgaa 361 tggacaagtt gtgtgcgctg ttcctcacaa cttagagagt aaatctccag ctgccccggg 421 tcagagtcca acagggagtg tatgctacga acgtaatggg gaagtgtttt atctgactta 481 cacccctgaa gatgtcgaag ggaacgttca gctggaaact ggagataaaa taaactttgt 541 aattgataac aataaacata ctggtgctgt aagtgctcgc aacattatgc tgttgaaaaa 601 gaaacaagcc cgctgtcagg gagtagtttg tgccatgaag gaggcatttg gctttattga 661 aagaggtgat gttgtaaaag agatattctt tcactatagt gaatttaagg gtgacttaga 721 aaccttacag cctggcgatg atgtggaatt cacaatcaag gacagaaatg gtaaagaagt 781 tgcaacagat gtcagactat tgcctcaagg aacagtcatt tttgaagata tcagcattga 841 acattttgaa ggaactgtaa ccaaagttat cccaaaagta cccagtaaaa accagaatga 901 cccattgcca ggacgcatca aagttgactt tgtgatccct aaagaacttc cctttggaga 961 caaagatacg aaatccaagg tgaccctgct ggaaggtgac catgttaggt ttaatatttc 1021 aacagaccga cgtgacaaat tagagcgagc aaccaatata gaagttctgt caaatacatt 1081 tcagttcact aatgaagccc gagaaatggg tgtgattgct gccatgagag atggttttgg 1141 tttcatcaag tgtgtggatc gtgatgttcg tatgttcttc cacttcagtg aaattctgga 1201 tgggaaccag ctccatattg cagatgaagt agagtttact gtggttcctg atatgctctc 1261 tgctcaaaga aatcatgcta ttaggattaa aaaacttccc aagggcacgg tttcatttca 1321 ttcccattca gatcaccgtt ttctgggcac ggtagaaaaa gaagccactt tttccaatcc 1381 taaaaccact agcccaaata aaggcaaaga gaaggaggct gaggatggca ttattgctta 1441 tgatgactgt ggggtgaaac tgactattgc ttttcaagcc aaggatgtgg aaggatctac 1501 ttctcctcaa ataggagata aggttgaatt tagtattagt gacaaacaga ggcctggaca 1561 gcaggttgca acttgtgtgc gacttttagg tcgtaattct aactccaaga ggctcttggg 1621 ttatgtggca actctgaagg ataattttgg atttattgaa acagccaatc atgataagga 1681 aatctttttc cattacagtg agttctctgg tgatgttgat agcctggaac tgggggacat 1741 ggtcgagtat agcttgtcca aaggcaaagg caacaaagtc agtgcagaaa aagtgaacaa 1801 aacacactca gtgaatggca ttactgagga agctgatccc accatttact ctggcaaagt 1861 aattcgcccc ctgaggagtg ttgatccaac acagactgag taccaaggaa tgattgagat 1921 tgtggaggag ggcgatatga aaggtgaggt ctatccattt ggcatcgttg ggatggccaa 1981 caaaggggat tgcctgcaga aaggggagag cgtcaagttc caattgtgtg tcctgggcca 2041 aaatgcacaa actatggctt acaacatcac acccctgcgc agggccacag tggaatgtgt 2101 gaaagatcag tttggcttca ttaactatga agtaggagat agcaagaagc tctttttcca 2161 tgtgaaagaa gttcaggatg gcattgagct acaggcagga gatgaggtgg agttctcagt 2221 gattcttaat cagcgcactg gcaagtgcag cgcctgtaat gtttggcgag tctgtgaggg 2281 ccccaaggct gttgcagctc ctcgacctga tcggttggtc aatcgcttga agaatatcac 2341 tctggatgat gccagtgctc ctcgcctaat ggttcttcgt cagccaaggg gaccagataa 2401 ctcaatgggg tttggtgcag aaagaaagat ccgtcaagct ggtgtcattg attaactcga 2461 ggaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc 2521 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat 2581 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt 2641 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa 2701 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat 2761 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc 2821 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt 2881 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct 2941 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct 3001 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 3061 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg 3121 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 3181 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 3241 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 3301 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 3361 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata 3421 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 3481 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca 3541 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 3601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 3661 aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca 3721 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 3781 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 3841 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 3901 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 3961 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 4021 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 4081 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 4141 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 4201 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 4261 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 4321 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 4381 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 4441 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 4501 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 4561 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 4621 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 4681 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 4741 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 4801 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 4861 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 4921 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 4981 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 5041 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 5101 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 5161 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 5221 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 5281 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 5341 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 5401 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 5461 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 5521 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca 5581 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 5641 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 5701 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 5761 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 5821 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 5881 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc 5941 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc 6001 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg 6061 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa 6121 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg 6181 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg 6241 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc 6301 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg 6361 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc 6421 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga 6481 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc 6541 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg 6601 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct 6661 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg 6721 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg 6781 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg 6841 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga 6901 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc 6961 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg 7021 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct 7081 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc 7141 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac 7201 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgaa //