LOCUS LT726827 9444 bp DNA circular SYN 06-FEB-2017 DEFINITION Yeast expression vector pAS2-mRIP1-ID-DD, complete sequence. ACCESSION LT726827 VERSION LT726827.1 KEYWORDS . SOURCE Yeast expression vector pAS2-mRIP1-ID-DD ORGANISM Yeast expression vector pAS2-mRIP1-ID-DD other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9444) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..9444 /organism="Yeast expression vector pAS2-mRIP1-ID-DD" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 6212." /db_xref="taxon:1945464" rep_origin complement(2..982) /note="2mu ori" regulatory complement(1060..1164) /note="FLP terminator" /regulatory_class="terminator" CDS 1884..2558 /transl_table=11 /note="TRP1" /protein_id="SJL86609.1" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK" rep_origin complement(2661..3116) /note="f1 ori" regulatory complement(3312..3586) /note="CYC1 terminator" /regulatory_class="terminator" CDS complement(3855..4304) /transl_table=11 /note="CYH2" /protein_id="SJL86610.1" /translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA" regulatory 5075..5478 /note="ADH1 promoter" /regulatory_class="promoter" CDS 5501..>5941 /transl_table=11 /note="GALbd" /protein_id="SJL86611.1" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS" misc_feature 5957..5983 /note="HA epitope" CDS 5987..7048 /transl_table=11 /note="mRIP1-ID-DD" /protein_id="SJL86612.1" /translation="MLKKEYPDQSPVLQRMFSLQHDCVPLPPSRSNSEQPGSLHSSQG LQMGPVEESWFSSSPEYPQDENDRSVQAKLQEEASYHAFGIFAEKQTKPQPRQNEAYN REEERKRRVSHDPFAQQRARENIKSAGARGHSDPSTTSRGIAVQQLSWPATQTVWNNG LYNQHGFGTTGTGVWYPPNLSQMYSTYKTPVPETNIPGSTPTMPYFSGPVADDLIKYT IFNSSGIQIGNHNYMDVGLNSQPPNNTCKEESTSRHQAIFDNTTSLTDEHLNPIRENL GRQWKNCARKLGFTESQIDEIDHDYERDGLKEKVYQMLQKWLMREGTKGATVGKLAQA LHQCCRIDLLNHLIRASQS" regulatory 7082..7183 /note="ADH1 terminator" /regulatory_class="terminator" regulatory complement(7336..7419) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(7511..7913) /note="pMB1 ori" CDS complement(8454..9314) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL86613.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 2687 a 1998 c 2124 g 2635 t ORIGIN 1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5221 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcatgt taaagaaaga 6001 gtatccagat caaagcccag tgctgcagag aatgttttca ctgcagcatg actgtgtacc 6061 cttacctccg agcaggtcaa attcagaaca acctggatcg ctgcacagtt cccaggggct 6121 ccagatgggt cctgtggagg agtcctggtt ttcttcctcc ccagagtacc cacaggacga 6181 gaatgatcgc agtgtgcagg ctaagctgca agaggaagcc agctatcatg cttttggaat 6241 atttgcagag aaacagacaa aaccgcagcc aaggcagaat gaggcttaca acagagagga 6301 ggaaaggaaa cgaagggtct ctcatgaccc ctttgcacag cagagagctc gtgagaatat 6361 taagagtgca ggagcaagag gtcattctga tcccagcaca acgagtcgtg gaattgcagt 6421 gcaacagctg tcatggccag ccacccaaac agtttggaac aatggattgt ataatcagca 6481 tggatttgga actacaggta caggagtttg gtatccgcca aatctaagcc aaatgtatag 6541 tacttataaa actccagtgc cagagaccaa cataccggga agcacaccca ccatgccata 6601 cttctctggg ccagtagcag atgacctcat aaaatatact atattcaata gttctggtat 6661 tcagattgga aaccacaatt atatggatgt tggactgaat tcacaaccac caaacaatac 6721 ttgcaaagaa gagtcgactt ccagacacca agccatcttt gataacacca ctagtctgac 6781 tgatgaacac ctgaacccta tcagggaaaa cctgggaagg cagtggaaaa actgtgcccg 6841 caagctgggc ttcactgagt ctcagatcga tgaaatcgac catgactatg aaagagatgg 6901 actgaaagag aaagtttacc aaatgcttca gaagtggctg atgcgggaag gcaccaaagg 6961 ggccacagtg ggaaagttgg cccaggcact tcaccaatgt tgcaggatag acctgctgaa 7021 ccacttgatt cgtgccagcc agagctaagg atccgtcgac ctgcagccaa gctaattccg 7081 ggcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt 7141 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttgttct tgagtaactc 7201 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac 7261 ctctaccggc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 7321 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 7381 gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 7441 ggaaacctgt cgtgccagct ggattaatga atcggccaac gcgcggggag aggcggtttg 7501 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 7561 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 7621 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 7681 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 7741 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 7801 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 7861 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 7921 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 7981 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 8041 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 8101 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 8161 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 8221 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 8281 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 8341 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 8401 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 8461 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 8521 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 8581 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 8641 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 8701 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 8761 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 8821 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 8881 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 8941 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 9001 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 9061 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 9121 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 9181 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 9241 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 9301 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 9361 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 9421 acatttcccc gaaaagtgcc acct //