LOCUS LT726824 6395 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mRip2, complete sequence.
ACCESSION LT726824
VERSION LT726824.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mRip2
ORGANISM Mammalian expression vector pCAGGS-E-mRip2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6395)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6395
/organism="Mammalian expression vector pCAGGS-E-mRip2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 6121."
/db_xref="taxon:1944813"
misc_feature 16..54
/note="E-tag"
CDS 64..1683
/transl_table=11
/note="mRIP2"
/protein_id="SJL86599.1"
/translation="MNGDAICSALPPIPYHKLADLHYLSRGASGTVSSARHADWRVRV
AVKHLHIHTPLLDSERNDILREAEILHKARFSYILPILGICNEPEFLGIVTEYMPNGS
LNELLHRKTEYPDIAWPLRFRILHEIALGVNYLHNMNPPLLHHDLKTQNILLDNEFHV
KIADFGLSKWRMMSLSQSRSYKSAPEGGTIIYMPPENYEPGQKSRASVKHDIYSYAVI
MWEVLSRKQPFEEVTNPLQIMYSVSQGHRPDTSEENLPFDIPHRGLMISLIQSGWAQN
PDERPSFLKCLIELEPVLRTFEDITFLEAVIQLKKAKIQSSSSTIHLCDKKMDLSLNI
PANHPPQEESCGSSLLSRNTGSPGPSRSLSAPQDKGFLSGAPQDCSSLKAHHCPGNHS
WDGIVSVPPGAAFCDRRASSCSLAVISPFLVEKGSERPPIGIAQQWIQSKREAIVSQM
TEACLNQSLDALLSRDLIMKEDYELISTKPTRTSKVRQLLDTSDIQGEEFAKVVVQKL
KDNKQLGLQPYPEVPVLSKAPPSNFPQNKSL"
regulatory 1685..1776
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1777..2133
/note="3' flanking region of rabbit beta-globin"
regulatory complement(2211..2293)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2435..2510
/note="SV40 ori"
regulatory complement(2541..2615)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 2541..2615
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2730..3131)
/note="pMB1 ori"
CDS complement(3672..4532)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86600.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4668..5045
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5051..6392
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5420..6344
/note="intron AG promoter"
BASE COUNT 1464 a 1663 c 1687 g 1581 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gctatgaacg gggacgccat ctgcagcgcg ctacccccca tcccgtacca caagctcgcc
121 gacctgcact acctcagccg gggcgcctct ggcacggtct cgtcggcacg acacgcagac
181 tggcgcgtgc gggtggcagt gaagcaccta cacatccaca cgccgctgct cgacagtgaa
241 agaaatgata tcttacgaga agccgaaata ttacataaag caagatttag ttacattctt
301 ccaattttgg gaatttgcaa tgagcctgaa tttttgggca tagttactga atacatgcca
361 aatggatcat taaatgagct tctacacaga aaaactgaat atcctgatat tgcatggcca
421 ttgagattcc gcatcctgca tgaaattgct cttggtgtaa attatctgca caatatgaac
481 ccccctctgc ttcatcatga cctaaagact cagaatatct tgttggataa tgaatttcat
541 gttaagattg cagattttgg tttatcaaaa tggcgcatga tgtcactctc tcaatcacga
601 agttacaaat ctgcacccga aggaggaaca atcatctata tgccacctga gaactatgag
661 ccaggacaga aatcaagggc cagtgtgaag catgatatat acagctatgc agttatcatg
721 tgggaagtgt tatccagaaa acagcctttt gaagaggtca ccaatccttt gcagatcatg
781 tacagtgtgt cccagggaca tcgacctgac accagtgagg agaatttgcc atttgatata
841 cctcatcgag gtctcatgat ctctctaata cagagtggat gggcgcaaaa cccagatgaa
901 agaccatcct ttttgaaatg cttaatagaa cttgaaccag ttctgagaac atttgaagac
961 ataactttcc ttgaggctgt tattcagcta aagaaagcaa agatacagag ttcctcaagt
1021 actattcact tatgtgataa gaaaatggat ttatcgctaa acatacctgc aaatcatccc
1081 ccacaggagg aatcgtgtgg atcctctctg ctctctagaa acactggttc tcctggaccc
1141 tccagatccc tatcagctcc tcaagacaag ggatttttat ctggagcacc tcaagactgc
1201 tcttcgttga aggcacacca ctgtcctgga aaccacagct gggatggtat cgtttctgta
1261 ccaccagggg cagccttctg tgatcgcagg gcctcctcgt gttccttggc tgtaataagt
1321 ccattcttgg ttgagaaagg ttcagagcgc ccaccaattg gtatagccca acagtggatc
1381 cagagtaaga gggaagccat tgtgagccag atgactgaag cctgccttaa ccaatcgctg
1441 gacgcccttc tgtctcgtga cctgatcatg aaggaggact atgaactcat tagcaccaaa
1501 cctacaagga cctcaaaagt cagacagctg ctagatacgt ctgacatcca aggagaagag
1561 tttgccaaag ttgtagtgca gaagttgaaa gataacaagc agctggggct gcagccttac
1621 ccagaagtac ctgtgctttc caaagcaccg ccttcaaatt tccctcagaa taaaagcttg
1681 tgaagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
1741 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
1801 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
1861 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
1921 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
1981 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
2041 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
2101 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt
2161 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
2221 tacgagccgg aagcataaag tgtaaagcct gggtgcctaa tgagtgagct aactcacatt
2281 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg
2341 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
2401 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
2461 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
2521 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca
2581 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
2641 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg
2701 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
2761 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
2821 atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
2881 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
2941 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
3001 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
3061 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
3121 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
3181 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
3241 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
3301 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
3361 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
3421 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
3481 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
3541 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
3601 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
3661 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
3721 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
3781 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
3841 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
3901 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
3961 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
4021 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
4081 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
4141 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
4201 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
4261 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
4321 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
4381 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
4441 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
4501 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
4561 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
4621 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt
4681 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
4741 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
4801 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
4861 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
4921 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
4981 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
5041 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
5101 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
5161 gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
5221 gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
5281 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg
5341 ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
5401 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
5461 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
5521 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
5581 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
5641 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
5701 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
5761 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
5821 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
5881 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
5941 cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
6001 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
6061 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
6121 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
6181 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
6241 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
6301 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
6361 tggttattgt gctgtctcat cattttggca aagaa
//