LOCUS LT726818 9033 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pFB-Neo-E-hA20, complete sequence.
ACCESSION LT726818
VERSION LT726818.1
KEYWORDS .
SOURCE Mammalian expression vector pFB-Neo-E-hA20
ORGANISM Mammalian expression vector pFB-Neo-E-hA20
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9033)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9033
/organism="Mammalian expression vector pFB-Neo-E-hA20"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5942."
/db_xref="taxon:1945027"
regulatory 169..760
/note="5' MMLV-LTR"
/regulatory_class="promoter"
misc_feature 761..2046
/note="psi+ extended MMLV packaging signal"
misc_feature 2077..2115
/note="E-tag"
CDS 2125..4497
/transl_table=11
/note="hA20"
/protein_id="SJL86584.1"
/translation="MAEQVLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIHHFKTMH
RYTLEMFRTCQFCPQFREIIHKALIDRNIQATLESQKKLNWCREVRKLVALKTNGDGN
CLMHATSQYMWGVQDTDLVLRKALFSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD
TRNWNDEWDNLIKMASTDTPMARSGLQYNSLEEIHIFVLCNILRRPIIVISDKMLRSL
ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSHHFVPLVTLKDSGPEIRAVPLV
NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLMVIEIPVQGWDHGTTHLINAAKLDEAN
LPKEINLVDDYFELVQHEYKKWQENSEQGRREGHAQNPMEPSVPQLSLMDVKCETPNC
PFFMSVNTQPLCHECSERRQKNQNKLPKLNSKPGPEGLPGMALGASRGEAYEPLAWNP
EESTGGPHSAPPTAPSPFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHNARQLHASH
APDHTRHLDPGKCQACLQDVTRTFNGICSTCFKRTTAEASSSLSTSLPPSCHQRSKSD
PSRLVRSPSPHSCHRAGNDAPAGCLSQAARTPGDRTGTSKCRKAGCVYFGTPENKGFC
TLCFIEYRENKHFAAASGKVSPTASRFQNTIPCLGRECGTLGSTMFEGYCQKCFIEAQ
NQRFHEAKRTEEQLRSSQRRDVPRTTQSTSRPKCARASCKNILACRSEELCMECQHPN
QRMGPGAHRGEPAPEDPPKQRCRAPACDHFGNAKCNGYCNECFQFKQMYG"
misc_feature 4498..4541
/note="3' UTR of hA20"
misc_feature 4555..5053
/note="5' UTR of EMC-POLYP (IRES)"
CDS 5225..6019
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL86585.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 6079..6672
/note="3' MMLV-LTR"
/regulatory_class="terminator"
rep_origin complement(7025..7427)
/note="pMB1 ori"
CDS complement(7968..8828)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86586.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2165 a 2520 c 2309 g 2039 t
ORIGIN
1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
121 ccctattccc cacactcacc ggagccaaag ccgcgggaca tatacatgtg aaagacccca
181 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
241 aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc
301 caaagcggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
361 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
421 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
481 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
541 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
601 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
661 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
721 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
781 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta
841 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta
901 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc
961 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg
1021 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc ccccttagag gagggatatg
1081 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
1141 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc
1201 tctgtctgac tgtgtttctg tatttgtctg aaaatatggg cccgggccag actgttacca
1261 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcagatcgct cacaaccagt
1321 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
1381 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
1441 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
1501 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
1561 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
1621 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga
1681 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta
1741 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
1801 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt
1861 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct
1921 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg
1981 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta
2041 gactgccgga tcgaattgtc gacgaattcc accatgggtg cgccggtgcc gtatccggac
2101 ccgctggaac cgcgtgcggc cgcaatggct gaacaagtcc ttcctcaggc tttgtatttg
2161 agcaatatgc ggaaagctgt gaagatacgg gagagaactc cagaagacat ttttaaacct
2221 actaatggga tcattcatca ttttaaaacc atgcaccgat acacactgga aatgttcaga
2281 acttgccagt tttgtcctca gtttcgggag atcatccaca aagccctcat cgacagaaac
2341 atccaggcca ccctggaaag ccagaagaaa ctcaactggt gtcgagaagt ccggaagctt
2401 gtggcgctga aaacgaacgg tgacggcaat tgcctcatgc atgccacttc tcagtacatg
2461 tggggcgttc aggacacaga cttggtactg aggaaggcgc tgttcagcac gctcaaggaa
2521 acagacacac gcaactttaa attccgctgg caactggagt ctctcaaatc tcaggaattt
2581 gttgaaacgg ggctttgcta tgatactcgg aactggaatg atgaatggga caatcttatc
2641 aaaatggctt ccacagacac acccatggcc cgaagtggac ttcagtacaa ctcactggaa
2701 gaaatacaca tatttgtcct ttgcaacatc ctcagaaggc caatcattgt catttcagac
2761 aaaatgctaa gaagtttgga atcaggttcc aatttcgccc ctttgaaagt gggtggaatt
2821 tacttgcctc tccactggcc tgcccaggaa tgctacagat accccattgt tctcggctat
2881 gacagccatc attttgtacc cttggtgacc ctgaaggaca gtgggcctga aatccgagct
2941 gttccacttg ttaacagaga ccggggaaga tttgaagact taaaagttca ctttttgaca
3001 gatcctgaaa atgagatgaa ggagaagctc ttaaaagagt acttaatggt gatagaaatc
3061 cccgtccaag gctgggacca tggcacaact catctcatca atgccgcaaa gttggatgaa
3121 gctaacttac caaaagaaat caatctggta gatgattact ttgaacttgt tcagcatgag
3181 tacaagaaat ggcaggaaaa cagcgagcag gggaggagag aggggcacgc ccagaatccc
3241 atggaacctt ccgtgcccca gctttctctc atggatgtaa aatgtgaaac gcccaactgc
3301 cccttcttca tgtctgtgaa cacccagcct ttatgccatg agtgctcaga gaggcggcaa
3361 aagaatcaaa acaaactccc aaagctgaac tccaagccgg gccctgaggg gctccctggc
3421 atggcgctcg gggcctctcg gggagaagcc tatgagccct tggcgtggaa ccctgaggag
3481 tccactgggg ggcctcattc ggccccaccg acagcaccca gcccttttct gttcagtgag
3541 accactgcca tgaagtgcag gagccccggc tgccccttca cactgaatgt gcagcacaac
3601 ggattttgtg aacgttgcca caacgcccgg caacttcacg ccagccacgc cccagaccac
3661 acaaggcact tggatcccgg gaagtgccaa gcctgcctcc aggatgttac caggacattt
3721 aatgggatct gcagtacttg cttcaaaagg actacagcag aggcctcctc cagcctcagc
3781 accagcctcc ctccttcctg tcaccagcgt tccaagtcag atccctcgcg gctcgtccgg
3841 agcccctccc cgcattcttg ccacagagct ggaaacgacg cccctgctgg ctgcctgtct
3901 caagctgcac ggactcctgg ggacaggacg gggacgagca agtgcagaaa agccggctgc
3961 gtgtattttg ggactccaga aaacaagggc ttttgcacac tgtgtttcat cgagtacaga
4021 gaaaacaaac attttgctgc tgcctcaggg aaagtcagtc ccacagcgtc caggttccag
4081 aacaccattc cgtgcctggg gagggaatgc ggcacccttg gaagcaccat gtttgaagga
4141 tactgccaga agtgtttcat tgaagctcag aatcagagat ttcatgaggc caaaaggaca
4201 gaagagcaac tgagatcgag ccagcgcaga gatgtgcctc gaaccacaca aagcacctca
4261 aggcccaagt gcgcccgggc ctcctgcaag aacatcctgg cctgccgcag cgaggagctc
4321 tgcatggagt gtcagcatcc caaccagagg atgggccctg gggcccaccg gggtgagcct
4381 gcccccgaag acccccccaa gcagcgttgc cgggcccccg cctgtgatca ttttggcaat
4441 gccaagtgca acggctactg caacgaatgc tttcagttca agcagatgta tggctaaccg
4501 gaaacaggtg ggtcacctcc tgcaagaagt ggggcctcga gcggccgcga tccggttatt
4561 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
4621 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
4681 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
4741 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
4801 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
4861 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
4921 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
4981 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
5041 acacgatcga taatatggaa caaaaactta tttctgaaga agacttggac accaaacttt
5101 cctgccgctc gatttctcca cccaggtgaa ctcctccctc acctccccga cggggcgagg
5161 ctccatggct gccccctcgc tgcacccgtc cctggtgcca cgcggttcca tgggatcgtt
5221 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
5281 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
5341 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
5401 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
5461 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
5521 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
5581 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
5641 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
5701 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
5761 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
5821 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
5881 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
5941 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
6001 tcttgacgag ttcttctgag cgggactctg gggttcgata aaataaaaga ttttatttag
6061 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
6121 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
6181 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
6241 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
6301 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
6361 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
6421 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
6481 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
6541 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
6601 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
6661 gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc
6721 caccaccggg aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
6781 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
6841 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
6901 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
6961 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
7021 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
7081 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
7141 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
7201 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
7261 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
7321 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
7381 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
7441 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
7501 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
7561 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
7621 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
7681 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
7741 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
7801 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
7861 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
7921 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
7981 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
8041 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
8101 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
8161 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
8221 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
8281 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
8341 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
8401 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
8461 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
8521 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
8581 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
8641 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
8701 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
8761 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
8821 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
8881 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
8941 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
9001 aataggcgta tcacgaggcc ctttcgtctt caa
//