LOCUS LT726815 5926 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-hA20-Ct-ZF1, complete
sequence.
ACCESSION LT726815
VERSION LT726815.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-hA20-Ct-ZF1
ORGANISM Mammalian expression vector pCAGGS-E-hA20-Ct-ZF1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5926)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5926
/organism="Mammalian expression vector
pCAGGS-E-hA20-Ct-ZF1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5928."
/db_xref="taxon:1944765"
misc_feature 16..54
/note="E-tag"
CDS 64..1122
/transl_table=11
/note="hA20 fragment"
/protein_id="SJL86578.1"
/translation="MGEAYEPLAWNPEESTGGPHSAPPTAPSPFLFSETTAMKCRSPG
CPFTLNVQHNGFCERCHNARQLHASHAPDHTRHLDPGKCQACLQDVTRTFNGICSTCF
KRTTAEASSSLSTSLPPSCHQRSKSDPSRLVRSPSPHSCHRAGNDAPAGCLSQAARTP
GDRTGTSKCRKAGCVYFGTPENKGFCTLCFIEYRENKHFAAASGKVSPTASRFQNTIP
CLGRECGTLGSTMFEGYCQKCFIEAQNQRFHEAKRTEEQLRSSQRRDVPRTTQSTSRP
KCARASCKNILACRSEELCMECQHPNQRMGPGAHRGEPAPEDPPKQRCRAPACDHFGN
AKCNGYCNECFQFKQMYG"
regulatory 1216..1307
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1308..1664
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1742..1824)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1966..2041
/note="SV40 ori"
regulatory complement(2072..2146)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 2072..2146
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2261..2662)
/note="pMB1 ori"
CDS complement(3203..4063)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86579.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4199..4576
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4582..5923
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4951..5875
/note="intron AG promoter"
BASE COUNT 1263 a 1626 c 1646 g 1391 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcgatgggag aagcctatga gcccttggcg tggaaccctg aggagtccac tggggggcct
121 cattcggccc caccgacagc acccagccct tttctgttca gtgagaccac tgccatgaag
181 tgcaggagcc ccggctgccc cttcacactg aatgtgcagc acaacggatt ttgtgaacgt
241 tgccacaacg cccggcaact tcacgccagc cacgccccag accacacaag gcacttggat
301 cccgggaagt gccaagcctg cctccaggat gttaccagga catttaatgg gatctgcagt
361 acttgcttca aaaggactac agcagaggcc tcctccagcc tcagcaccag cctccctcct
421 tcctgtcacc agcgttccaa gtcagatccc tcgcggctcg tccggagccc ctccccgcat
481 tcttgccaca gagctggaaa cgacgcccct gctggctgcc tgtctcaagc tgcacggact
541 cctggggaca ggacggggac gagcaagtgc agaaaagccg gctgcgtgta ttttgggact
601 ccagaaaaca agggcttttg cacactgtgt ttcatcgagt acagagaaaa caaacatttt
661 gctgctgcct cagggaaagt cagtcccaca gcgtccaggt tccagaacac cattccgtgc
721 ctggggaggg aatgcggcac ccttggaagc accatgtttg aaggatactg ccagaagtgt
781 ttcattgaag ctcagaatca gagatttcat gaggccaaaa ggacagaaga gcaactgaga
841 tcgagccagc gcagagatgt gcctcgaacc acacaaagca cctcaaggcc caagtgcgcc
901 cgggcctcct gcaagaacat cctggcctgc cgcagcgagg agctctgcat ggagtgtcag
961 catcccaacc agaggatggg ccctggggcc caccggggtg agcctgcccc cgaagacccc
1021 cccaagcagc gttgccgggc ccccgcctgt gatcattttg gcaatgccaa gtgcaacggc
1081 tactgcaacg aatgctttca gttcaagcag atgtatggct aactcgaggt cgaggaattc
1141 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
1201 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
1261 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
1321 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
1381 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
1441 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
1501 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
1561 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
1621 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc
1681 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
1741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc
1801 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
1861 cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
1921 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
1981 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
2041 tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta
2101 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
2161 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga
2221 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
2281 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
2341 gtaatacggt tatccacaga atcagggata acgcaggaaa gaacatgtga gcaaaaggcc
2401 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
2461 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
2521 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
2581 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
2641 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
2701 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
2761 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
2821 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
2881 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
2941 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
3001 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
3061 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
3121 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
3181 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
3241 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
3301 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
3361 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
3421 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
3481 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
3541 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
3601 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
3661 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
3721 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
3781 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
3841 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
3901 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
3961 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
4021 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
4081 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
4141 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
4201 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
4261 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
4321 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
4381 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
4441 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
4501 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
4561 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
4621 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
4681 ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga ggggcggggc
4741 ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
4801 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
4861 gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
4921 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
4981 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
5041 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
5101 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
5161 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
5221 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
5281 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
5341 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
5401 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
5461 ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg
5521 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
5581 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
5641 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
5701 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
5761 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
5821 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
5881 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaa
//