LOCUS       LT726811                5000 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Mammalian expression vector pCAGGS-FLAG-hUb-K48R, complete
            sequence.
ACCESSION   LT726811
VERSION     LT726811.1
KEYWORDS    .
SOURCE      Mammalian expression vector pCAGGS-FLAG-hUb-K48R
  ORGANISM  Mammalian expression vector pCAGGS-FLAG-hUb-K48R
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Mammalian expression vector
                     pCAGGS-FLAG-hUb-K48R"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 5902."
                     /db_xref="taxon:1944839"
     misc_feature    13..36
                     /note="FLAG epitope"
     CDS             46..276
                     /transl_table=11
                     /note="hUBB mutated mature sequence"
                     /protein_id="SJL86570.1"
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI
                     FAGRQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     misc_feature    187..189
                     /note="K48R"
     regulatory      286..377
                     /note="3' UTR of rabbit beta-globin, incl. polyA"
                     /regulatory_class="terminator"
     misc_feature    378..734
                     /note="3' flanking region of rabbit beta-globin"
     regulatory      complement(812..894)
                     /note="lac promoter"
                     /regulatory_class="promoter"
     rep_origin      1036..1111
                     /note="SV40 ori"
     regulatory      complement(1142..1216)
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      1142..1216
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     rep_origin      complement(1331..1732)
                     /note="pMB1 ori"
     CDS             complement(2273..3133)
                     /transl_table=11
                     /note="ampicillin resistance"
                     /protein_id="SJL86571.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      3273..3650
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     regulatory      3656..4997
                     /note="AG promoter"
                     /regulatory_class="promoter"
     misc_feature    4025..4949
                     /note="intron AG promoter"
BASE COUNT         1065 a         1327 c         1391 g         1217 t
ORIGIN      
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgcgatgca gatcttcgtg
       61 aaaaccctga ccggcaagac catcactctg gaggtggagc ccagtgacac catcgaaaat
      121 gtgaaggcca agatccaaga taaagaaggc atcccccccg accagcagag gctcatcttt
      181 gcaggcaggc agctggaaga tggccgcact ctttctgact acaacatcca gaaagagtcg
      241 accctgcacc tggtcctgcg tctcagaggt gggtaggtcg acgaagatct ttttccctct
      301 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
      361 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
      421 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
      481 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
      541 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
      601 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
      661 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
      721 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
      781 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      841 gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      901 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa
      961 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     1021 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     1081 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     1141 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1201 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1261 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1321 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1381 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcagggata
     1441 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1681 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1741 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1801 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1861 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1921 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1981 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2041 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2401 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3241 catttccccg aaaagtgcca cctgggtcga tcgacattga ttattgacta gttattaata
     3301 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     3361 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     3421 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     3481 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     3541 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     3601 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
     3661 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
     3721 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc
     3781 cccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc
     3841 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
     3901 gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt
     3961 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
     4021 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
     4081 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt
     4141 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
     4201 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct
     4261 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg
     4321 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     4381 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc
     4441 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
     4501 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
     4561 ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
     4621 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
     4681 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
     4741 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     4801 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
     4861 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     4921 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     4981 ctcatcattt tggcaaagaa
//