LOCUS LT726807 6125 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian vector pdcDNA-R4R3-dhCMV, complete sequence.
ACCESSION LT726807
VERSION LT726807.1
KEYWORDS .
SOURCE Mammalian vector pdcDNA-R4R3-dhCMV
ORGANISM Mammalian vector pdcDNA-R4R3-dhCMV
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6125)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6125
/organism="Mammalian vector pdcDNA-R4R3-dhCMV"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 8998."
/db_xref="taxon:1945174"
regulatory complement(18..142)
/note="attR4"
/regulatory_class="attenuator"
CDS complement(183..488)
/transl_table=11
/note="ccdB"
/protein_id="SJL86555.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(830..1489)
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL86556.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
regulatory complement(1538..1590)
/note="lacUV5 promoter"
/regulatory_class="promoter"
regulatory 1598..1722
/note="attR3"
/regulatory_class="attenuator"
regulatory complement(1753..1769)
/note="SP6 promoter"
/regulatory_class="promoter"
regulatory 1795..2019
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 2065..2493
/note="f1 ori"
regulatory 2498..2707
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 2664..2739
/note="SV40 ori"
CDS 2831..3625
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL86557.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 3799..3873
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3799..3873)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(4012..4095)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(4187..4589)
/note="pMB1 ori"
CDS complement(5130..5990)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86558.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1474 a 1557 c 1489 g 1605 t
ORIGIN
1 gacggatcgg gagatcttca actttgtata gaaaagttga acgagaaacg taaaatgata
61 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
121 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac
181 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg
241 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc
301 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacgggg gactttatct
361 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata
421 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta
481 aactgcattt caccagcccc ggttctcgtc ggcaaaagag ccgttcattt caataaaccg
541 ggcgacctca gccatccctt cttgattttc cgctttccag cgttcggcac gcagacgacg
601 ggcttcattc tgcatggttg tgcttaccga accggagata ctgacatcat atatgccttg
661 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc
721 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc
781 gcaaatacgc atactgttat ctggctttta gtaagccgga tcctctagat tacgccccgc
841 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
901 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
961 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
1021 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
1081 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
1141 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
1201 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
1261 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
1321 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
1381 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
1441 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
1501 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
1561 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
1621 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
1681 atcattttac gtttctcgtt caactttatt atacatagtt gagctcgagc atgcatctag
1741 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
1801 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
1861 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
1921 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
1981 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
2041 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
2101 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
2161 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
2221 gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
2281 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
2341 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
2401 tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa
2461 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
2521 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
2581 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
2641 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
2701 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
2761 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
2821 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
2881 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
2941 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
3001 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
3061 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
3121 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
3181 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
3241 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
3301 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
3361 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
3421 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
3481 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
3541 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
3601 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
3661 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
3721 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
3781 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
3841 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
3901 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
3961 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
4021 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
4081 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
4141 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
4201 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
4261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
4321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
4381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
4441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
4501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
4561 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
4621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
4681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
4741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
4801 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
4861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
4921 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
4981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
5041 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
5101 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
5161 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
5221 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
5281 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
5341 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
5401 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
5461 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
5521 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
5581 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
5641 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
5701 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
5761 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
5821 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
5881 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
5941 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
6001 tttcaatatt attgagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
6061 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
6121 acgtc
//