LOCUS LT726806 8807 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pRMCE-DV1, complete sequence.
ACCESSION LT726806
VERSION LT726806.1
KEYWORDS .
SOURCE Mammalian expression vector pRMCE-DV1
ORGANISM Mammalian expression vector pRMCE-DV1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8807)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8807
/organism="Mammalian expression vector pRMCE-DV1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 8870."
/db_xref="taxon:1945060"
misc_feature 35..277
/note="chicken beta-globin 5'HS4 insulator core sequence"
misc_feature 278..520
/note="chicken beta-globin 5'HS4 insulator core sequence"
regulatory 539..1046
/note="mPGK1 promoter"
/regulatory_class="promoter"
misc_feature 1070..1117
/note="FRT mutant"
regulatory complement(1205..1288)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(1380..1782)
/note="pMB1 ori"
CDS complement(2323..3183)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86551.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 3801..3848
/note="FRT"
misc_feature 3865..3968
/note="SA"
misc_feature complement(4031..4064)
/note="loxP"
regulatory 4751..4847
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4751..4847)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 5005..5101
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5005..5101)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 5259..5355
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5259..5355)
/note="SV40 polyA late"
/regulatory_class="terminator"
misc_feature complement(5444..5477)
/note="loxP"
regulatory complement(5533..5657)
/note="attR1"
/regulatory_class="attenuator"
regulatory 5665..5717
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 5766..6425
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL86552.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 6767..7072
/transl_table=11
/note="ccdB"
/protein_id="SJL86553.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 7113..7237
/note="attR2"
/regulatory_class="attenuator"
misc_feature 7281..7775
/note="5' UTR of the EMCV polyprotein gene (IRES)"
CDS 7788..8507
/transl_table=11
/note="EGFP"
/protein_id="SJL86554.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
regulatory 8538..8762
/note="BGH polyA"
/regulatory_class="terminator"
BASE COUNT 2160 a 2287 c 2195 g 2165 t
ORIGIN
1 cgcgccgggc cctcgcgact cgaggaataa attcgagctc acggggacag ccccccccca
61 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg
121 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc
181 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg
241 agcctgcaga cacctggggg atacggggaa aaagcttgag ctcacgggga cagccccccc
301 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc
361 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac
421 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct
481 ttgagcctgc agacacctgg gggatacggg gaaaaagctt gtcgacgaat aaattcaatt
541 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
601 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
661 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
721 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
781 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
841 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
901 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
961 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
1021 ctcttcctca tctccgggcc tttcgacctg caatcgccgc caccatggag aagttcctat
1081 tccgaagttc ctattcttca aaaggtatag gaacttccga tcgggatccc caatcctgca
1141 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
1201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
1261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
1321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
1381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
1441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
1501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
1561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
1621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
1681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
1741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
1801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
1861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
1921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
1981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
2041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
2101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
2161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
2221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
2281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
2341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
2401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
2461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
2521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
2581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
2641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
2701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
2761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
2821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
2881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
2941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
3001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
3061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
3121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
3181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
3241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
3301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
3361 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
3541 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
3601 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
3661 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
3721 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
3781 attccacaaa agtgcgatcg gaagttccta ttccgaagtt cctattctct agaaagtata
3841 ggaacttctt aattaaggga tctgtagggc gcagtagtcc agggtttcct tgatgatgtc
3901 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc
3961 tttccagtgg ggatcgacgg tatcgtagag tcgaggccgc tctagaacta gtggatccgg
4021 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg
4081 caggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
4141 gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
4201 ccaccggtag cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
4261 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
4321 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
4381 ggtaggatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc
4441 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt
4501 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca
4561 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat
4621 cagaagcttg cagatctgcg actctagagg atctgcgact ctagaggatc ataatcagcc
4681 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
4741 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
4801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
4861 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctgc gactctagag
4921 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
4981 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
5041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
5101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
5161 ctgcgactct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
5221 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
5281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
5341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
5401 tatcatgtct ggatccccat caagctgatc cggaaccctt aatataactt cgtataatgt
5461 atgctatacg aagttattag gtccctcgac ctgcagccca agctagctta tcgataccgt
5521 cgacctcgaa tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat
5581 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
5641 catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg
5701 gctcgtataa tgtgtggatt ttgagttagg atccggcgag attttcagga gctaaggaag
5761 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
5821 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
5881 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
5941 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
6001 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
6061 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
6121 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
6181 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
6241 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
6301 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc tgtgatggct
6361 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
6421 cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
6481 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggtg
6541 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
6601 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
6661 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
6721 attgaaatga acggctcttt tgctgacgag aacagggact ggtgaaatgc agtttaaggt
6781 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
6841 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
6901 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
6961 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
7021 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
7081 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
7141 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
7201 tttacgtttc tcgttcagct ttcttgtaca aagtggtgat tcgaggtcga cggtatcgat
7261 aagcttgata tcgaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc
7321 cgcttggaat aaggccggtg agcgtttgtc tatatgttat tttccaccat attgccgtct
7381 tttggcaatg tgagggcccg aaacctggcc ctgtcttctt gacgacgatt cctaggggtc
7441 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
7501 tggaagcttc ttgaagacaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
7561 acctggcgac atggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
7621 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
7681 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg
7741 ggacgtggtt ttggtttgaa aaacacgatg ataatatggc cacaaccatg gtgagcaagg
7801 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
7861 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
7921 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
7981 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
8041 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
8101 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
8161 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
8221 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
8281 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
8341 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
8401 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
8461 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgagc
8521 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
8581 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
8641 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
8701 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
8761 ggcttctgag gcggaaagaa ccagctgggg ctcgatcctc tagttgg
//