LOCUS LT726805 5169 bp DNA circular SYN 13-NOV-2018
DEFINITION Shuttle vector pMG14, complete sequence.
ACCESSION LT726805
VERSION LT726805.1
KEYWORDS .
SOURCE Shuttle vector pMG14
ORGANISM Shuttle vector pMG14
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5169)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
REFERENCE 3
AUTHORS Gimpel M., Brantl S.
TITLE Construction of a modular plasmid family for chromosomal
integration in Bacillus subtilis
JOURNAL J. Microbiol. Methods 91(2), 312-317(2012).
PUBMED 22982324
FEATURES Location/Qualifiers
source 1..5169
/organism="Shuttle vector pMG14"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 8465."
/db_xref="taxon:1945224"
regulatory 5..234
/note="cggR promoter"
/regulatory_class="promoter"
misc_feature 262..302
/note="5' UTR of gapA"
misc_feature 311..334
/note="Strep-tag II"
CDS complement(<470..1009)
/transl_table=11
/note="amyE fragment"
/protein_id="SJL86547.1"
/translation="MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAETANKSNELTA
PSIKSGTILHAWNWSFNTLKHNMKDIHDAGYTAIQTSPINQVKEGNQGDKSMSNWYWL
YQPTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHTTSDYAAISNEVKSIPN
WTHGNTQIKNWSDRWDVTQN"
misc_feature complement(1010..1125)
/note="5' UTR of amyE"
CDS 1334..2194
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86548.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 2735..3137
/note="pMB1 ori"
regulatory 3229..3312
/note="lac promoter"
/regulatory_class="promoter"
misc_feature 3313..3350
/note="5' UTR of lacZ, incl. RBS"
CDS complement(<3396..>4199)
/codon_start=1
/transl_table=11
/note="amyE fragment"
/protein_id="SJL86549.1"
/translation="STWMSDDDIRLGWAVIASRSGSTPLFFSRPEGGGNGVRFPGKSQ
IGDRGSALFEDQAITAVNRFHNVMAGQPEELSNPNGNNQIFMNQRGSHGVVLANAGSS
SVSINTATKLPDGRYDNKAGAGSFQVNDGKLTGTINARSVAVLYPDDIAKAPHVFLEN
YKTGVTHSFNDQLTITLRADANTTKAVYQINNGPETAFKDGDQFTIGKGDPFGKTYTI
MLKGTNSDGVTRTEKYSFVKRDPASAKTIGYQNPNHWSQVNAYIYKHDGS"
CDS complement(4289..5059)
/transl_table=11
/note="kanamycin/tobramycin resistance (aadD)"
/protein_id="SJL86550.1"
/translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLG
RQTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLT
HGQFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQG
PTTFLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSG
QLSDSEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
BASE COUNT 1379 a 1209 c 1120 g 1461 t
ORIGIN
1 aattctttgg ataaaatgaa aacgctttaa tgaaacagcc ctttctacgg gaagggctgt
61 ttatattggg atgcaccatt tggcgctttc tgtataagat aaagatatat aggataaaat
121 attgctggat aaaacgacgc ggcatgaaaa ctctgcgaat attgtcgatg aattggctct
181 taacagttga ataaacaatt ccaccctgtt aaaataatta aagaaagcag aaatctgcag
241 aagttattaa gggatgaata atccctcaat ataaatatct ctcacttatt taaaggagga
301 aagcttcatg tggagccacc cgcagttcga aaaaggtacc cggggatcct ctagagtcct
361 gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag
421 gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttaa ttctgcgtga
481 catcccatcg atcagaccag tttttaattt gtgtgtttcc atgtgtccag tttggaatac
541 tcttaacctc attggaaatc gcggcataat cactggtggt atgattgatg accgcgtcaa
601 caatgacctt tatgccatat tcttcagcgg ctgcacacat ttctttaaat tcttgttcag
661 tacctaagta acggttgcca atttgatacg atgtcggctg atacagccag taccagttcg
721 acatgctttt atctccttga ttcccttcct ttacttggtt aatcggagat gtctgaatgg
781 ctgtatatcc tgcatcatga atatccttca tattgtgttt taacgtattg aacgaccaat
841 tccatgcatg aagaatggtt ccgcttttga tcgacggtgc tgtaagctca ttcgatttgt
901 tcgccgtttc agcactcgca gccgccggtc ctgccagaac caaatgaaac agcaataaaa
961 atccagcgaa taacggcagt aaagaggttt tgaatcgttt tgcaaacatt cttgacactc
1021 cttatttgat tttttgaaga cttacttcgg agtcaaaaat ccctcttact tcattcttcc
1081 gcttcctcct ttcaaaccga tgtgaagact ggagaatttt gttaattctt gaagacgaaa
1141 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
1201 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
1261 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
1321 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
1381 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
1441 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
1501 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
1561 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
1621 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
1681 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
1741 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
1801 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
1861 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
1921 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
1981 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
2041 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
2101 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
2161 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
2221 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
2281 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
2341 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
2401 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
2461 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
2521 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
2581 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
2641 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
2701 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
2761 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
2821 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
2881 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
2941 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
3001 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
3061 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
3121 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
3181 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
3241 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
3301 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
3361 ttacgccaag ctaatcggcg gccgcatcga tgcaggctcc catcatgttt atagatataa
3421 gcatttacct ggctccaatg attcggattt tgatagccga tggttttggc cgacgctgga
3481 tctcttttaa caaaactgta tttctcggtc ctcgttacac catcactgtt cgttcctttt
3541 aacatgatgg tgtatgtttt gccaaatgga tctccttttc cgattgtgaa ttgatctcca
3601 tccttaaacg ccgtctctgg tccattattg atttgataaa cggcttttgt tgtattcgca
3661 tctgcacgca aggtaatcgt cagttgatca ttgaaagaat gtgttacacc tgttttgtaa
3721 ttctcaagga aaacatgagg cgcttttgca atatcatcag gataaagcac agctacagac
3781 ctggcattga tcgtgcctgt cagtttacca tcgttcactt gaaatgaacc cgctccagct
3841 ttattgtcat acctgccatc aggcaatttt gttgccgtat tgatagagac agaggatgaa
3901 cctgcatttg ccagcacaac gccatgtgag ccgcgctgat tcataaatat ctggttgttt
3961 ccattcgggt tcgagagttc ctcaggctgt ccagccatca cattgtgaaa tctattgacc
4021 gcagtgatag cctgatcttc aaataaagca ctcccgcgat cgcctatttg gcttttcccc
4081 gggaacctca caccatttcc gcctccctca ggtctggaaa agaaaagagg cgtactgcct
4141 gaacgagaag ctatcaccgc ccagcctaaa cggatatcat catcgctcat ccatgtcgac
4201 gcgccatgac agccatgata attactaata ctaggagaag ttaataaata cgtaaccaac
4261 atgattaaca attattagag gtcatcgttc aaaatggtat gcgttttgac acatccacta
4321 tatatccgtg tcgttctgtc cactcctgaa tcccattcca gaaattctct agcgattcca
4381 gaagtttctc agagtcggaa agttgaccag acattacgaa ctggcacaga tggtcataac
4441 ctgaaggaag atctgattgc ttaactgctt cagttaagac cgaagcgctc gtcgtataac
4501 agatgcgatg atgcagacca atcaacatgg cacctgccat tgctacctgt acagtcaagg
4561 atggtagaaa tgttgtcggt ccttgcacac gaatattacg ccatttgcct gcatattcaa
4621 acagctcttc tacgataagg gcacaaatcg catcgtggaa cgtttgggct tctaccgatt
4681 tagcagtttg atacactttc tctaagtatc cacctgaatc ataaatcggc aaaatagaga
4741 aaaattgacc atgtgtaagc ggccaatctg attccacctg agatgcataa tctagtagaa
4801 tctcttcgct atcaaaattc acttccacct tccactcacc ggttgtccat tcatggctga
4861 actctgcttc ctctgttgac atgacacaca tcatctcaat atccgaatag ggcccatcag
4921 tctgacgacc aagagagcca taaacaccaa tagccttaac atcatcccca tatttatcca
4981 atattcgttc cttaatttca tgaacaatct tcattctttc ttctctagtc attattattg
5041 gtccattcac tattctcatt cccttttcag ataattttag atttgctttt ctaaataaga
5101 atatttggag agcaccgttc ttattcagct attaaaccca ttatatcggg tttttgaggg
5161 gatttcaag
//