LOCUS LT726802 7475 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian vector pCR3-EGFPC-GW, complete sequence. ACCESSION LT726802 VERSION LT726802.1 KEYWORDS . SOURCE Mammalian vector pCR3-EGFPC-GW ORGANISM Mammalian vector pCR3-EGFPC-GW other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7475) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7475 /organism="Mammalian vector pCR3-EGFPC-GW" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 8375." /db_xref="taxon:1945167" regulatory 10..593 /note="hCMV-IE promoter and enhancer" /regulatory_class="promoter" regulatory 638..654 /note="T7 promoter" /regulatory_class="promoter" regulatory complement(722..846) /note="attR1" /regulatory_class="attenuator" regulatory 854..906 /note="lacUV5 promoter" /regulatory_class="promoter" CDS 955..1614 /transl_table=11 /note="chloramphenicol resistance" /protein_id="SJL86532.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" CDS 1956..2261 /transl_table=11 /note="ccdB" /protein_id="SJL86533.1" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" regulatory 2302..2426 /note="attR2" /regulatory_class="attenuator" CDS 2458..3174 /transl_table=11 /note="EGFP" /protein_id="SJL86534.1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITLGMDELYK" regulatory complement(3192..3208) /note="SP6 promoter" /regulatory_class="promoter" regulatory 3234..3458 /note="BGH polyA" /regulatory_class="terminator" rep_origin complement(3482..3808) /note="pMB1 ori" regulatory complement(4526..4595) /note="HSV-TK polyA" /regulatory_class="terminator" CDS complement(4786..5574) /transl_table=11 /note="Tn5 neomycin resistance" /protein_id="SJL86535.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" rep_origin complement(5634..5709) /note="SV40 ori" regulatory complement(5666..5947) /note="SV40 early promoter" /regulatory_class="promoter" CDS complement(6026..6886) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL86536.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7018..7473 /note="f1 ori" BASE COUNT 1845 a 1914 c 1900 g 1816 t ORIGIN 1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg 241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 661 cccaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc 721 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt 781 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc 841 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat 901 gtgtggattt tgagttagga tccggcgaga ttttcaggag ctaaggaagc taaaatggag 961 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 1021 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg 1081 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 1141 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg 1201 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt 1261 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa 1321 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg 1381 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 1441 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag 1501 gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc 1561 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct 1621 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt 1681 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc 1741 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt 1801 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga 1861 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa 1921 cggctctttt gctgacgaga acagggactg gtgaaatgca gtttaaggtt tacacctata 1981 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg 2041 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg 2101 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg 2161 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg 2221 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tccgttatac 2281 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta 2341 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct 2401 cgttcagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgagcgtg 2461 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 2521 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 2581 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 2641 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 2701 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 2761 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 2821 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 2881 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 2941 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 3001 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 3061 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 3121 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaatctaga 3181 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc 3241 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 3301 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 3361 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 3421 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 3481 tggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 3541 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3601 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3661 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3721 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3781 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 3841 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 3901 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 3961 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 4021 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 4081 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 4141 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 4201 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 4261 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4321 gtatatatga gtaacctgag gctatggcag ggcctgccgc cccgacgttg gctgcgagcc 4381 ctgggccttc acccgaactt ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt 4441 ggccccaatg gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc 4501 gtttatgaac aaacgaccca acaccgtgcg ttttattctg tctttttatt gccgtcatag 4561 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc ctagggtggg 4621 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa 4681 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca 4741 ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc 4801 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag 4861 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat 4921 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc 4981 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc 5041 gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc 5101 ttgatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg 5161 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc 5221 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc 5281 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc 5341 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc 5401 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag 5461 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag 5521 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa 5581 cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc 5641 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa 5701 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg 5761 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc 5821 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc 5881 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat 5941 tccacagctg gttctttccg cctcaggact cttccttttt caataaatca atctaaagta 6001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 6061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 6121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 6301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 6361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 6421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 6541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 6721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 6841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 6901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 6961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 7021 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 7081 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 7141 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 7201 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 7261 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 7321 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 7381 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 7441 cgaattttaa caaaatatta acgcttacaa tttac //