LOCUS LT726799 6448 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pGFP-N-GW, complete sequence.
ACCESSION LT726799
VERSION LT726799.1
KEYWORDS .
SOURCE Vector pGFP-N-GW
ORGANISM Vector pGFP-N-GW
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6448)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6448
/organism="Vector pGFP-N-GW"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 8367."
/db_xref="taxon:1945317"
rep_origin 39..365
/note="pMB1 ori"
regulatory 372..939
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
CDS 984..>1700
/transl_table=11
/note="EGFP"
/protein_id="SJL86518.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
regulatory complement(1719..1843)
/note="attR1"
/regulatory_class="attenuator"
regulatory 1851..1903
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 1952..2611
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL86519.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 2953..3258
/transl_table=11
/note="ccdB"
/protein_id="SJL86520.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 3299..3423
/note="attR2"
/regulatory_class="attenuator"
regulatory 3585..3681
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3585..3681)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3735..4190)
/note="f1 ori"
regulatory 4191..4286
/note="ampicillin resistance promoter"
/regulatory_class="promoter"
misc_feature 4287..4321
/note="5' UTR of amp, incl. RBS"
regulatory 4342..4623
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4580..4655
/note="SV40 ori"
CDS 4715..5509
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL86521.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 5700..5769
/note="HSV-TK polyA"
/regulatory_class="terminator"
BASE COUNT 1625 a 1631 c 1649 g 1543 t
ORIGIN
1 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
61 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
121 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
181 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
241 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
301 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
361 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat
421 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
481 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
541 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
601 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
661 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
721 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
781 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
841 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
901 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
961 cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
1021 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
1081 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
1141 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
1201 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
1261 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
1321 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
1381 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
1441 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
1501 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
1561 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
1621 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
1681 gcatggacga gctgtacaag tccggactca gatcatcaac aagtttgtac aaaaaagctg
1741 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
1801 agactacata atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac
1861 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc
1921 ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
1981 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
2041 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
2101 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
2161 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
2221 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
2281 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
2341 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
2401 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
2461 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
2521 ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
2581 gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag
2641 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
2701 tacccgaagt atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac
2761 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
2821 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
2881 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
2941 gggactggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
3001 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
3061 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
3121 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
3181 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
3241 atgttctggg gaatataaat gtcaggctcc gttatacaca gccagtctgc aggtcgacca
3301 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
3361 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
3421 ggttgatgat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga
3481 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
3541 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
3601 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
3661 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
3721 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
3781 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
3841 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
3901 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
3961 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
4021 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
4081 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
4141 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
4201 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
4261 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
4321 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
4381 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
4441 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
4501 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
4561 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
4621 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
4681 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
4741 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
4801 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
4861 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
4921 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
4981 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
5041 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
5101 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
5161 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
5221 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
5281 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
5341 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
5401 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
5461 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
5521 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
5581 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
5641 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
5701 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
5761 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
5821 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
5881 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
5941 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
6001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
6061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
6121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
6181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
6241 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
6301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
6361 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
6421 cagcggtcgg gctgaacggg gggttcgt
//