LOCUS LT726797 6402 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pDsRed-N-GW, complete sequence.
ACCESSION LT726797
VERSION LT726797.1
KEYWORDS .
SOURCE Mammalian expression vector pDsRed-N-GW
ORGANISM Mammalian expression vector pDsRed-N-GW
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6402)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6402
/organism="Mammalian expression vector pDsRed-N-GW"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 8365."
/db_xref="taxon:1944965"
regulatory 109..205
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(109..205)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(259..714)
/note="f1 ori"
regulatory 715..810
/note="ampicillin resistance promoter"
/regulatory_class="promoter"
misc_feature 811..845
/note="5' UTR of amp, incl. RBS"
regulatory 866..1147
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 1104..1179
/note="SV40 ori"
CDS 1239..2033
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL86510.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 2224..2293
/note="HSV-TK polyA"
/regulatory_class="terminator"
rep_origin 3011..3337
/note="pMB1 ori"
regulatory 3344..3911
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
CDS 3956..>4630
/transl_table=11
/note="mDsRed"
/protein_id="SJL86511.1"
/translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTA
KLQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDG
GVVEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGE
ISHALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAE
ARHSGSQ"
regulatory complement(4694..4818)
/note="attR1"
/regulatory_class="attenuator"
regulatory 4826..4878
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 4927..5586
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL86512.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 5928..6233
/transl_table=11
/note="ccdB"
/protein_id="SJL86513.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 6274..6398
/note="attR2"
/regulatory_class="attenuator"
BASE COUNT 1603 a 1620 c 1645 g 1534 t
ORIGIN
1 gggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
61 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
121 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
181 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
241 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
301 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
361 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
421 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
481 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
541 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
601 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
661 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
721 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
781 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
841 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
901 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
961 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
1021 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
1081 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
1141 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
1201 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
1261 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
1321 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
1381 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
1441 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
1501 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
1561 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
1621 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
1681 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
1741 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
1801 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
1861 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
1921 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
1981 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
2041 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
2101 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
2161 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
2221 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
2281 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
2341 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
2401 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
2461 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
2521 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
2581 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
2641 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
2701 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
2761 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
2821 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
2881 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
2941 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
3001 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
3061 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
3121 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
3181 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
3241 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
3301 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
3361 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
3421 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
3481 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
3541 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
3601 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
3661 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
3721 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
3781 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
3841 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
3901 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatgga
3961 caacaccgag gacgtcatca aggagttcat gcagttcaag gtgcgcatgg agggctccgt
4021 gaacggccac tacttcgaga tcgagggcga gggcgagggc aagccctacg agggcaccca
4081 gaccgccaag ctgcaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
4141 cccccagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
4201 catgaagctg tccttccccg agggcttcac ctgggagcgc tccatgaact tcgaggacgg
4261 cggcgtggtg gaggtgcagc aggactcctc cctgcaggac ggcaccttca tctacaaggt
4321 gaagttcaag ggcgtgaact tccccgccga cggccccgta atgcagaaga agactgccgg
4381 ctgggagccc tccaccgaga agctgtaccc ccaggacggc gtgctgaagg gcgagatctc
4441 ccacgccctg aagctgaagg acggcggcca ctacacctgc gacttcaaga ccgtgtacaa
4501 ggccaagaag cccgtgcagc tgcccggcaa ccactacgtg gactccaagc tggacatcac
4561 caaccacaac gaggactaca ccgtggtgga gcagtacgag cacgccgagg cccgccactc
4621 cggctcccag tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac
4681 cgcgggccca tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
4741 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
4801 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
4861 ggctcgtata atgtgtggat tttgagttag gatccggcga gattttcagg agctaaggaa
4921 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
4981 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
5041 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
5101 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
5161 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
5221 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
5281 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
5341 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
5401 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
5461 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc
5521 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
5581 gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
5641 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
5701 gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
5761 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
5821 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
5881 tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg
5941 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
6001 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
6061 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
6121 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
6181 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
6241 gctccgttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
6301 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
6361 ttttacgttt ctcgttcagc tttcttgtac aaagtggttg at
//