LOCUS LT009442 8369 bp DNA circular SYN 15-AUG-2016
DEFINITION Lentiviral vector pDG1i-VSV-Blast, complete sequence.
ACCESSION LT009442
VERSION LT009442.1
KEYWORDS .
SOURCE Lentiviral vector pDG1i-VSV-Blast
ORGANISM Lentiviral vector pDG1i-VSV-Blast
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8369)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (22-DEC-2015) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Groote P., Grootjans S., Lippens S., Eichperger C., Leurs K.,
Kahr I., Tanghe G., Bruggeman I., De Schamphelaire W., Urwyler C.,
Vandenabeele P., Haustraete J., Declercq W.
TITLE Generation of a new Gateway-compatible inducible lentiviral vector
platform allowing easy derivation of co-transduced cells
JOURNAL BioTechniques 60(5), 252-259(2016).
PUBMED 27177818
FEATURES Location/Qualifiers
source 1..8369
/organism="Lentiviral vector pDG1i-VSV-Blast"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent University,
Belgium) accession number LMBP 9592."
/db_xref="taxon:1783449"
regulatory complement(35..159)
/note="gateway recombination site attR1"
/regulatory_class="attenuator"
CDS 268..927
/transl_table=11
/gene="cat"
/product="chloramphenicol acetyl transferase"
/note="chloramphenicol resistance"
/protein_id="CVH74057.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 1269..1574
/transl_table=11
/gene="ccdB"
/product="ccdB"
/note="B gene of the control of cell death locus of the
Escherichia coli F plasmid"
/protein_id="CVH74059.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 1615..1739
/note="gateway recombination site attR2"
/regulatory_class="attenuator"
misc_feature 1783..1815
/note="VSV epitope"
regulatory 1851..2133
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 2090..2165
/note="SV40 ori"
regulatory 2238..2303
/note="synthetic EM7 promoter"
/regulatory_class="promoter"
CDS 2304..2702
/transl_table=11
/gene="bsr"
/product="blasticidin S-resistance"
/protein_id="CVH74061.1"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 2772..2787
/note="HIV-1 cPPT"
regulatory 2788..3022
/note="RSV/HIV-1 dU3/3' LTR"
/regulatory_class="terminator"
regulatory 3094..3168
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3094..3168)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin 3304..3379
/note="SV40 ori"
regulatory complement(3413..3429)
/note="T7 promoter"
/regulatory_class="promoter"
rep_origin 3597..4052
/note="f1 origin"
CDS 4183..5043
/transl_table=11
/gene="bla"
/product="beta-lactamase"
/note="ampicillin resistance"
/protein_id="CVH74062.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 5584..5986
/note="pMB1 ori"
regulatory 6078..6161
/note="lac promoter"
/regulatory_class="promoter"
regulatory 6225..6241
/note="T3 promoter"
/regulatory_class="promoter"
regulatory 6350..6678
/note="RSV/HIV-1 5' LTR"
/regulatory_class="promoter"
misc_feature 6789..6833
/note="HIV-1 psi packaging signal"
misc_feature 7343..7576
/note="HIV-1 Rev response element"
regulatory 8094..8361
/note="hCMVTRE promoter"
/regulatory_class="promoter"
BASE COUNT 2264 a 1888 c 2091 g 2126 t
ORIGIN
1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg
61 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca
181 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
241 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
301 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
361 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
421 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
481 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
541 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
601 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
661 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
721 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
781 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
841 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
901 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
1021 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
1081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
1141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
1201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
1261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
1321 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
1381 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
1441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
1501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
1561 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
1621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
1681 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
1741 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtacaccga catcgagatg
1801 aacagactgg gcaagacgcg taccggttag taatgagttt ggaattaatt ctgtggaatg
1861 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
1921 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
1981 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
2041 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
2101 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
2161 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
2221 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
2281 acgacaaggt gaggaactaa accatggcca agcctttgtc tcaagaagaa tccaccctca
2341 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
2401 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
2461 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
2521 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
2581 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
2641 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
2701 aagcacaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
2761 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
2821 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
2881 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
2941 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
3001 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
3061 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
3121 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
3181 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
3241 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
3301 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
3361 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
3421 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
3481 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
3541 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
3601 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
3661 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
3721 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
3781 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
3841 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
3901 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
3961 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
4021 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
4081 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
4141 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
4201 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
4261 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
4321 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
4381 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
4441 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
4501 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
4561 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
4621 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
4681 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
4741 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
4801 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
4861 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
4921 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
4981 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
5041 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
5101 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
5161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
5221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
5281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
5341 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
5401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
5461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
5521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
5581 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
5641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
5701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
5761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
5821 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
5881 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
5941 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
6001 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
6061 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
6121 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
6181 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
6241 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
6301 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
6361 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
6421 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
6481 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
6541 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
6601 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
6661 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
6721 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
6781 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
6841 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
6901 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
6961 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
7021 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
7081 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
7141 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
7201 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
7261 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
7321 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
7381 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
7441 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
7501 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
7561 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
7621 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
7681 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
7741 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
7801 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
7861 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
7921 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
7981 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
8041 tagtgaacgg atctcgacgg tatcgatggt ccgaggttct agacgagttt actccctatc
8101 agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
8161 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
8221 gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
8281 tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc
8341 gtttagtgaa ccgtcagatc gcaccggtc
//