LOCUS LT009441 8382 bp DNA circular SYN 15-AUG-2016
DEFINITION Lentiviral vector pDG1i-V5-Blast, complete sequence.
ACCESSION LT009441
VERSION LT009441.1
KEYWORDS .
SOURCE Lentiviral vector pDG1i-V5-Blast
ORGANISM Lentiviral vector pDG1i-V5-Blast
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8382)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (22-DEC-2015) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Groote P., Grootjans S., Lippens S., Eichperger C., Leurs K.,
Kahr I., Tanghe G., Bruggeman I., De Schamphelaire W., Urwyler C.,
Vandenabeele P., Haustraete J., Declercq W.
TITLE Generation of a new Gateway-compatible inducible lentiviral vector
platform allowing easy derivation of co-transduced cells
JOURNAL BioTechniques 60(5), 252-259(2016).
PUBMED 27177818
FEATURES Location/Qualifiers
source 1..8382
/organism="Lentiviral vector pDG1i-V5-Blast"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent University,
Belgium) accession number LMBP 9591."
/db_xref="taxon:1783448"
regulatory complement(35..159)
/note="gateway recombination site attR1"
/regulatory_class="attenuator"
CDS 268..927
/transl_table=11
/gene="cat"
/product="chloramphenicol acetyl transferase"
/note="chloramphenicol resistance"
/protein_id="CVH74052.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 1267..1572
/transl_table=11
/gene="ccdB"
/product="ccdB"
/note="B gene of the control of cell death locus of the
Escherichia coli F plasmid"
/protein_id="CVH74054.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 1613..1737
/note="gateway recombination site attR2"
/regulatory_class="attenuator"
misc_feature 1790..1831
/note="V5 epitope"
regulatory 1864..2146
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 2103..2178
/note="SV40 ori"
regulatory 2251..2316
/note="synthetic EM7 promoter"
/regulatory_class="promoter"
CDS 2317..2715
/transl_table=11
/gene="bsr"
/product="blasticidin S-resistance"
/protein_id="CVH74055.1"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 2785..2800
/note="HIV-1 cPPT"
regulatory 2801..3035
/note="RSV/HIV-1 dU3/3' LTR"
/regulatory_class="terminator"
regulatory 3107..3181
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3107..3181)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin 3317..3392
/note="SV40 ori"
regulatory complement(3426..3442)
/note="T7 promoter"
/regulatory_class="promoter"
rep_origin 3610..4065
/note="f1 origin"
CDS 4196..5056
/transl_table=11
/gene="bla"
/product="beta-lactamase"
/note="ampicillin resistance"
/protein_id="CVH74056.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 5597..5999
/note="pMB1 ori"
regulatory 6091..6174
/note="lac promoter"
/regulatory_class="promoter"
regulatory 6238..6254
/note="T3 promoter"
/regulatory_class="promoter"
regulatory 6363..6691
/note="RSV/HIV-1 5' LTR"
/regulatory_class="promoter"
misc_feature 6802..6846
/note="HIV-1 psi packaging signal"
misc_feature 7356..7589
/note="HIV-1 Rev response element"
regulatory 8107..8374
/note="hCMVTRE promoter"
/regulatory_class="promoter"
BASE COUNT 2258 a 1897 c 2091 g 2136 t
ORIGIN
1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg
61 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca
181 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
241 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
301 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
361 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
421 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
481 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
541 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
601 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
661 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
721 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
781 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
841 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
901 tgcgatgagt ggcagggcgg ggcgtagatc tggatccggc ttactaaaag ccagataaca
961 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg
1021 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac
1081 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca
1141 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga
1201 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct
1261 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg
1321 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt
1381 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat
1441 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa
1501 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
1561 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga
1621 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
1681 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga
1741 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
1801 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag tttggaatta
1861 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag
1921 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
1981 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
2041 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
2101 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
2161 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
2221 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
2281 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa
2341 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
2401 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
2461 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
2521 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
2581 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
2641 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
2701 gtgtgggagg gctaagcaca attcgagctc ggtaccttta agaccaatga cttacaaggc
2761 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
2821 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
2881 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
2941 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
3001 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
3061 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
3121 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
3181 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
3241 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
3301 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
3361 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
3421 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
3481 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
3541 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
3601 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
3661 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
3721 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
3781 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
3841 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
3901 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
3961 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
4021 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
4081 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
4141 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
4201 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
4261 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
4321 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
4381 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
4441 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
4501 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
4561 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
4621 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
4681 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
4741 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
4801 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
4861 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
4921 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
4981 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
5041 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
5101 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
5161 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
5221 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
5281 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
5341 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
5401 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
5461 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
5521 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
5581 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
5641 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
5701 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
5761 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
5821 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
5881 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
5941 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
6001 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
6061 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
6121 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
6181 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
6241 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
6301 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
6361 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
6421 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
6481 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
6541 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
6601 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
6661 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
6721 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
6781 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
6841 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
6901 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
6961 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
7021 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
7081 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
7141 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
7201 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
7261 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
7321 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
7381 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
7441 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
7501 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
7561 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
7621 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
7681 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
7741 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
7801 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
7861 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
7921 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
7981 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
8041 agatccattc gattagtgaa cggatctcga cggtatcgat ggtccgaggt tctagacgag
8101 tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa
8161 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
8221 agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt
8281 tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat
8341 ataagcagag ctcgtttagt gaaccgtcag atcgcaccgg tc
//