LOCUS LT009438 9098 bp DNA circular SYN 15-AUG-2016 DEFINITION Lentiviral vector pDG1i-eGFP-V5-Blast, complete sequence. ACCESSION LT009438 VERSION LT009438.1 KEYWORDS . SOURCE Lentiviral vector pDG1i-eGFP-V5-Blast ORGANISM Lentiviral vector pDG1i-eGFP-V5-Blast other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9098) AUTHORS De Schamphelaire W. JOURNAL Submitted (22-DEC-2015) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Groote P., Grootjans S., Lippens S., Eichperger C., Leurs K., Kahr I., Tanghe G., Bruggeman I., De Schamphelaire W., Urwyler C., Vandenabeele P., Haustraete J., Declercq W. TITLE Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells JOURNAL BioTechniques 60(5), 252-259(2016). PUBMED 27177818 FEATURES Location/Qualifiers source 1..9098 /organism="Lentiviral vector pDG1i-eGFP-V5-Blast" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University, Belgium) accession number LMBP 7941" /db_xref="taxon:1783445" regulatory complement(35..159) /note="gateway recombination site attR1" /regulatory_class="attenuator" CDS 268..927 /transl_table=11 /gene="cat" /product="chloramphenicol acetyl transferase" /note="chloramphenicol resistance" /protein_id="CVH74026.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" CDS 1269..1574 /transl_table=11 /gene="ccdB" /product="ccdB" /note="B gene of the control of cell death locus of the Escherichia coli F plasmid" /protein_id="CVH74027.1" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" regulatory 1615..1739 /note="gateway recombination site attR2" /regulatory_class="attenuator" CDS 1771..2559 /transl_table=11 /gene="EGFP" /product="EGFP" /note="Aequorea victoria green fluorescent protein,enhanced red-shifted variant with V5 epitope tag" /protein_id="CVH74029.1" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKEGPRFEGKPIPNPLLGLDSTRTG" regulatory 2580..2862 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 2819..2894 /note="SV40 ori" regulatory 2967..3032 /note="synthetic EM7 promoter" /regulatory_class="promoter" CDS 3033..3431 /transl_table=11 /gene="bsr" /product="blasticidin S-resistance" /protein_id="CVH74031.1" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP GIKAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 3501..3516 /note="HIV-1 cPPT" regulatory 3517..3751 /note="RSV/HIV-1 dU3/3' LTR" /regulatory_class="terminator" regulatory 3823..3897 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(3823..3897) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin 4033..4108 /note="SV40 ori" regulatory complement(4142..4158) /note="T7 promoter" /regulatory_class="promoter" rep_origin 4326..4781 /note="f1 origin" CDS 4912..5772 /transl_table=11 /gene="bla" /product="beta-lactamase" /note="ampicillin resistance" /protein_id="CVH74034.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6313..6715 /note="pMB1 ori" regulatory 6807..6890 /note="lac promoter" /regulatory_class="promoter" regulatory 6954..6970 /note="T3 promoter" /regulatory_class="promoter" regulatory 7079..7407 /note="RSV/HIV-1 5' LTR" /regulatory_class="promoter" misc_feature 7518..7562 /note="HIV-1 psi packaging signal" misc_feature 8072..8305 /note="HIV-1 Rev response element" regulatory 8823..9090 /note="hCMVTRE promoter" /regulatory_class="promoter" BASE COUNT 2432 a 2135 c 2294 g 2237 t ORIGIN 1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg 61 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac 121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca 181 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg 241 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt 301 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt 361 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat 421 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg 481 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt 541 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac 601 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg 661 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg 721 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc 781 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt 841 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac 901 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa 961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc 1021 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg 1081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac 1141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg 1201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga 1261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg 1321 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca 1381 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg 1441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg 1501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt 1561 tctggggaat ataaatgtca ggctccgtta tacacagcca gtctgcaggt cgaccatagt 1621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt 1681 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt 1741 gatatccagc acagtggcgg ccgctcgagt atggtgagca agggcgagga gctgttcacc 1801 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 1861 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 1921 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 1981 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 2041 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 2101 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 2161 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 2221 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 2281 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 2341 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 2401 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 2461 actctcggca tggacgagct gtacaaggag ggcccgcggt tcgaaggtaa gcctatccct 2521 aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagtttg gaattaattc 2581 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt 2641 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 2701 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 2761 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 2821 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 2881 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 2941 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca 3001 tagtataata cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat 3061 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca 3121 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc 3181 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag 3241 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct 3301 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg 3361 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt 3421 gggagggcta agcacaattc gagctcggta cctttaagac caatgactta caaggcagct 3481 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 3541 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 3601 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 3661 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 3721 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 3781 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 3841 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 3901 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag 3961 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 4021 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 4081 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc 4141 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga 4201 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 4261 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 4321 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 4381 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 4441 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 4501 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 4561 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 4621 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 4681 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 4741 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga 4801 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 4861 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 4921 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 4981 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 5041 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 5101 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 5161 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 5221 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 5281 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 5341 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 5401 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 5461 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 5521 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 5581 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 5641 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 5701 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 5761 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 5821 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 5881 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 5941 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 6001 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 6061 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 6121 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 6181 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 6241 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 6301 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 6361 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 6421 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 6481 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 6541 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 6601 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 6661 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 6721 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 6781 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 6841 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 6901 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac 6961 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct 7021 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc 7081 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac 7141 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat 7201 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg 7261 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 7321 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 7381 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg 7441 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg 7501 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag 7561 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat 7621 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag 7681 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca 7741 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata 7801 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga 7861 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc 7921 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata 7981 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag 8041 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag 8101 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat 8161 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc 8221 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa 8281 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca 8341 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc 8401 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct 8461 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata 8521 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat 8581 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag 8641 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga 8701 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat 8761 ccattcgatt agtgaacgga tctcgacggt atcgatggtc cgaggttcta gacgagttta 8821 ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta 8881 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg 8941 atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact 9001 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa 9061 gcagagctcg tttagtgaac cgtcagatcg caccggtc //