LOCUS MUSDHFR 1499 bp mRNA linear ROD 24-SEP-2001
DEFINITION Mus musculus dihydrofolate reductase (dhfr) mRNA, complete cds.
ACCESSION L26316
VERSION L26316.1
KEYWORDS dihydrofolate reductase.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 91 to 217; 228 to 240; 318 to 343; 346 to 517)
AUTHORS Nunberg,J.H., Kaufman,R.J., Chang,A.C., Cohen,S.N. and Schimke,R.T.
TITLE Structure and genomic organization of the mouse dihydrofolate
reductase gene
JOURNAL Cell 19 (2), 355-364 (1980)
PUBMED 6244105
REFERENCE 2 (bases 28 to 986)
AUTHORS Setzer,D.R., McGrogan,M. and Schimke,R.T.
TITLE Nucleotide sequence surrounding multiple polyadenylation sites in
the mouse dihydrofolate reductase gene
JOURNAL J. Biol. Chem. 257 (9), 5143-5147 (1982)
PUBMED 6121807
REFERENCE 3 (bases 1 to 670)
AUTHORS Crouse,G.F., Simonsen,C.C., McEwan,R.N. and Schimke,R.T.
TITLE Structure of amplified normal and variant dihydrofolate reductase
genes in mouse sarcoma S180 cells
JOURNAL J. Biol. Chem. 257 (13), 7887-7897 (1982)
PUBMED 6282858
REFERENCE 4 (bases 71 to 528)
AUTHORS Simonsen,C.C. and Levinson,A.D.
TITLE Isolation and expression of an altered mouse dihydrofolate
reductase cDNA
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80 (9), 2495-2499 (1983)
PUBMED 6573667
REFERENCE 5 (bases 1 to 1499)
AUTHORS Schilling,L.J. and Farnham,P.J.
TITLE Transcriptional regulation of the dihydrofolate reductase/rep-3
locus
JOURNAL Crit. Rev. Eukaryot. Gene Expr. 4 (1), 19-53 (1994)
PUBMED 7987046
FEATURES Location/Qualifiers
source 1..1499
/organism="Mus musculus"
/mol_type="mRNA"
/db_xref="taxon:10090"
/cell_line="sarcoma cell line s180"
gene 1..1499
/gene="dhfr"
5'UTR 1..55
/gene="dhfr"
CDS 56..619
/gene="dhfr"
/codon_start=1
/product="dihydrofolate reductase"
/protein_id="AAA37523.1"
/translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSV
EGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIE
QPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLL
PEYPGVLSEVQEEKGIKYKFEVYEKKD"
3'UTR 620..1499
/gene="dhfr"
regulatory 1130..1140
/regulatory_class="polyA_signal_sequence"
/gene="dhfr"
regulatory 1478..1483
/regulatory_class="polyA_signal_sequence"
/gene="dhfr"
polyA_site 1495
/gene="dhfr"
BASE COUNT 498 a 300 c 336 g 365 t
ORIGIN
1 aacttgacgg caatcctagc gtgaaggctg gtaggatttt atccccgctg ccatcatggt
61 tcgaccattg aactgcatcg tcgccgtgtc ccaaaatatg gggattggca agaacggaga
121 cctaccctgg cctccgctca ggaacgagtt caagtacttc caaagaatga ccacaacctc
181 ttcagtggaa ggtaaacaga atctggtgat tatgggtagg aaaacctggt tctccattcc
241 tgagaagaat cgacctttaa aggacagaat taatatagtt ctcagtagag aactcaaaga
301 accaccacga ggagctcatt ttcttgccaa aagtttggat gatgccttaa gacttattga
361 acaaccggaa ttggcaagta aagtagacat ggtttggata gtcggaggca gttctgttta
421 ccaggaagcc atgaatcaac caggccacct tagactcttt gtgacaagga tcatgcagga
481 atttgaaagt gacacgtttt tcccagaaat tgatttgggg aaatataaac ttctcccaga
541 atacccaggc gtcctctctg aggtccagga ggaaaaaggc atcaagtata agtttgaagt
601 ctacgagaag aaagactaac aggaagatgc tttcaagttc tctgctcccc tcctaaagct
661 atgcattttt ataagaccat gggacttttg ctggctttag atctatgagt aattatttct
721 ttagggaggg gtagttggaa gaagtgtccg tcccgtgacc ctggggatgg aacctaagac
781 ccagtgcgtg ctgagcaaat gctatactgc tgagccaccc caaccctagt cccctatata
841 attctaaaca atatgttgtc attttcccag taattcaaca aggtatagta aaagtgtcca
901 agttatgtca tctgcataag gtctcagtgt cccctcccat gaggaacctc aagtggctcc
961 ccagcatatg cacagggtac tgtgtgtaca gagaccccag tgatgtagag cccctagagc
1021 atgagcagat gtgtgggctc ataaaagtgg agctaggcag gtaagccaaa gggcagaaac
1081 aggtatacaa gcagagctgg aactcagact ataaagaaaa ttccatcaaa agtagagact
1141 ggattattgt atgcacatca acaacttgca gcaaagctct gctcactcag acagaaaatc
1201 agtaaatgga gaactccatt gtgttccatg gagacgagag caggtggaag attatgtaag
1261 atctgaaaca ctgaaattgt ctgcttctca tcttcagtga gattccaaag gatagtacag
1321 tgacagaaca agaataggca ctctctacaa aaaaaagaaa gaaaaaacta agtaatagca
1381 agcataatag ctatcgttaa gaactcagag ataatgaatt gagaatggat actgcttgaa
1441 atgaaaattt aataagttag aaactaaact ttataaaaat aaaaaaatga gcattaaaa
//