LOCUS SYNPUC8V 2665 bp DNA circular SYN 26-JUL-1993
DEFINITION pUC8 cloning vector.
ACCESSION L08959
VERSION L08959.1
KEYWORDS .
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 2665)
AUTHORS Gilbert,W.
TITLE Obtained from VecBase 3.0
JOURNAL Unpublished
COMMENT These data and their annotation were supplied to GenBank by Will
Gilbert under the auspices of the GenBank Currator Program. pUC8 -
Cloning vector
ENTRY PUC8 #TYPE DNA CIRCULAR
TITLE pUC8 - Cloning vector
DATE 17-SEP-1986
#sequence 16-DEC-1986
ACCESSION VB0021
SOURCE artificial
REFERENCE
#number 1
#authors Vieira J., Messing J.
#journal Gene (1982) 19: 259-268
#comment see also 'note added in proof'
REFERENCE
#number 2
#authors Pouwels P.H., Enger-Valk B.E., Brammar W.J.
#book Cloning Vectors, Elsvier 1985 and supplements
#comment vector I-A-iv-20
COMMENT
Assembled from pUC18 and M13mp8 by F. Pfeiffer, MPI, Martinsried
Revised 16-DEC-1986 by F. Pfeiffer:
2287/8 'AT' to 'TA' to match revised sequence of PBR322
The strand shown contains the beta-galactosidase mRNA sequence
including the multiple cloning site of M13mp8.
KEYWORDS
CROSSREFERENCE
#complement
VecBase(3):pUC8c
#parent
VecBase(3):pUC7, VecBase(3):M13mp8, VecSource(3):bGal8
#brother
VecBase(3):pUC9
#offspring
VecBase(3):pUC12, VecBase(3):pEMBL8m, VecBase(3):pEMBL8p,
VecBase(3):pUC830, VecBase(3):pOM2, VecBase(3):pOM4,
VecBase(3):pOM8
VecBase(3):pUC81, VecBase(3):pUC82, VecBase(3):pIC7 PARENT
Features of pUC8 (2665 bp)
residue source
1- 426 6002-6427 M13mp8
1- 229 1069-1297 Lac-Operon
230- 265 1- 36 polylinker of M13mp8
268- 426 1303-1461 Lac-Operon
427- 526 2351-2252 (c) pBR322
527- 663 2210-2074 (c) pBR322
664-2665 4355-2354 (c) pBR322
Conflict (cfl) and Mutations (mut):
pUC8 source
mut 1107 T C 3912 (c) pBR322
mut 1408 A G 3611 (c) pBR322
FEATURE
931-1719 1-789 Ap-R; b-lactamase
POLYLINKER EcoRI-SmaI-BamHI-SalI-PstI-HindIII
SELECTION
#resistance Ap
#indicator beta-galactosidase
SUMMARY pUC8 #length 2665 #checksum 2136.
FEATURES Location/Qualifiers
source 1..2665
/organism="synthetic construct"
/mol_type="genomic DNA"
/db_xref="taxon:32630"
BASE COUNT 654 a 680 c 670 g 661 t
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcccggg
241 gatccgtcga cctgcagcca agcttggcac tggccgtcgt tttacaacgt cgtgactggg
301 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
361 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
421 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
481 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
541 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
601 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
661 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
721 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
781 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
841 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
901 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
961 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
1021 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
1081 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
1141 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
1201 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
1261 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
1321 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
1381 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
1441 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
1501 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
1561 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
1621 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
1681 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
1741 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
1801 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
1861 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
1921 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
1981 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
2041 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
2101 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
2161 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
2221 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
2281 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
2341 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
2401 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
2461 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
2521 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
2581 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
2641 agtcagtgag cgaggaagcg gaaga
//