LOCUS SYNPGEM2V 2869 bp DNA circular SYN 26-JUL-1993 DEFINITION pGEM2 cloning vector Gemini 2 from Promega Biotec. ACCESSION L08871 VERSION L08871.1 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct other sequences; artificial sequences. REFERENCE 1 (bases 1 to 2869) AUTHORS Gilbert,W. TITLE Obtained from VecBase 3.0 JOURNAL Unpublished COMMENT These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. pGEM2 - Cloning Vector Gemini 2 from Promega Biotec ENTRY PGEM2 #TYPE DNA CIRCULAR TITLE pGEM2 - Cloning Vector Gemini 2 from Promega Biotec DATE 27-MAR-1986 #sequence 11-DEC-1986 #sequence 16-DEC-1986 ACCESSION VB0037 SOURCE artificial REFERENCE #number 1 #citation Promega technical Bulletin #comment The sequence was revised by Promega Biotec JUL-1986 Promega also confirmed the revision of DEC-1986 #comment copyright of the sequence by Promega COMMENT Entered by William Gilbert, Whitaker College, MIT and by J. Soppa, and F. Pfeiffer, MPI, Martinsried Revised 11-DEC-1986 by F. Pfeiffer according to Promega Revised 16-DEC-1986 by F. Pfeiffer: 534/5 'AT' to 'TA' to match revised sequence of PBR322 COMMENT The pGEM2 vector contains both the T7 and SP6 RNA polymerase promoters seperated by a short piece of DNA containing 11 unique restriction sites. It was constructed from pSP65 and a bacteriophage T7 promoter. During construction of the SP6 vectors, the majority of the lacZ gene was deleted. Therefore, the X-gal color screening method for the identification of recombinants is not possible with this vector. KEYWORDS CROSSREFERENCE #parent VecBase(3):pSP65 #parent VecBase(3):pUC13, VecSource(3):bGal13, VecSource(3):PromSP6, VecSource(3):PromT7, VecBase(3):pBR322 #brother VecBase(3):pGEM1 #offspring VecBase(3):pGEM4 PARENT Features of pGEM2 (2869 bp) residue source 10- 60 51- 1 (c) M13mp11/pUC13-polylinker 68- 87 20- 1 (c) T7 promoter 98- 156 59- 1 (c) pUC13 157-2345 2680- 492 (c) pUC13 2346-2624 654- 376 (c) pBR322 2625-2869 xxxx-xxxx phage SP6 2851-2869 1- 19 SP6 promoter Conflict (cfl) and Mutations (mut): none PARENT Features of pGEM2 (2869 bp) residue source 10- 60 51- 1 (c) M13mp11/pUC13-polylinker 68- 87 20- 1 (c) T7 promoter 98-2158 620-2680 pUC13c 2159-2345 1- 187 pUC13c 2346-2624 654- 376 (c) pBR322 2625-2869 xxxx-xxxx phage SP6 2851-2869 1- 19 SP6 promoter Conflict (cfl) and Mutations (mut): none FEATURE 1 start of SP6-RNA synthesis 69 (c) start of T7-RNA synthesis 1101-1889 789-1 (c) Ap-R; b-lactamase POLYLINKER HindIII-PstI-SalI-XbaI-BamHI-SmaI-SacI-EcoRI SELECTION #resistance Ap SUMMARY pGEM2 #length 2869 #checksum 1597. FEATURES Location/Qualifiers source 1..2869 /organism="synthetic construct" /mol_type="genomic DNA" /db_xref="taxon:32630" BASE COUNT 711 a 742 c 727 g 689 t ORIGIN 1 gaatacacgg aattcgagct cgcccgggga tcctctagag tcgacctgca gcccaagctt 61 ccggtctccc tatagtgagt cgtattaatt tcgataagcc agctgcatta atgaatcggc 121 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 1021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 1081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 1141 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 1201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 1261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 1321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 1381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 1441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 1501 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 1561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 1621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 1681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 1741 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 1801 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 1861 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 1921 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 1981 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 2041 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 2101 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 2161 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 2221 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 2281 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 2341 catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga 2401 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc 2461 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt 2521 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg 2581 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta gcgatgaccc 2641 tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga aactcagaag 2701 gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc 2761 agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat 2821 taatacataa ccttatgtat catacacata cgatttaggt gacactata //