LOCUS       SYNPGEM2V               2869 bp    DNA     circular SYN 26-JUL-1993
DEFINITION  pGEM2 cloning vector Gemini 2 from Promega Biotec.
ACCESSION   L08871
VERSION     L08871.1
KEYWORDS    .
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Gilbert,W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished
COMMENT     These data and their annotation were supplied to GenBank by Will
            Gilbert under the auspices of the GenBank Currator Program. pGEM2 -
            Cloning Vector Gemini 2 from Promega Biotec ENTRY PGEM2
            #TYPE DNA CIRCULAR
            TITLE pGEM2 - Cloning Vector Gemini 2 from Promega Biotec DATE
            27-MAR-1986
               #sequence  11-DEC-1986
               #sequence  16-DEC-1986
            ACCESSION VB0037
            SOURCE    artificial
            REFERENCE
               #number 1
               #citation  Promega technical Bulletin
               #comment   The sequence was revised by Promega Biotec JUL-1986
                          Promega also confirmed the revision of DEC-1986
               #comment  copyright of the sequence by Promega
            COMMENT
               Entered by William Gilbert, Whitaker College, MIT
                   and by J. Soppa, and F. Pfeiffer, MPI, Martinsried
               Revised 11-DEC-1986 by F. Pfeiffer according to Promega
               Revised 16-DEC-1986 by F. Pfeiffer:
               534/5 'AT' to 'TA' to match revised sequence of PBR322 COMMENT
               The pGEM2 vector contains both the T7 and SP6 RNA polymerase
            promoters
               seperated by a short piece of DNA containing 11 unique
            restriction sites.
               It was constructed from pSP65 and a bacteriophage T7 promoter.
               During construction of the SP6 vectors, the majority of the lacZ
            gene
               was deleted.  Therefore, the X-gal color screening method for
            the
               identification of recombinants is not possible with this vector.
            KEYWORDS
            CROSSREFERENCE
               #parent
                 VecBase(3):pSP65
               #parent
                 VecBase(3):pUC13, VecSource(3):bGal13,
                 VecSource(3):PromSP6, VecSource(3):PromT7,
                 VecBase(3):pBR322
               #brother
                 VecBase(3):pGEM1
               #offspring
                 VecBase(3):pGEM4
            PARENT
               Features of pGEM2 (2869 bp)
                 residue     source
                 10-  60    51-   1 (c) M13mp11/pUC13-polylinker
                 68-  87    20-   1 (c) T7 promoter
                 98- 156    59-   1 (c) pUC13
                157-2345  2680- 492 (c) pUC13
               2346-2624   654- 376 (c) pBR322
               2625-2869  xxxx-xxxx     phage SP6
               2851-2869     1-  19     SP6 promoter
               Conflict (cfl) and Mutations (mut): none
            PARENT
               Features of pGEM2 (2869 bp)
                 residue     source
                 10-  60    51-   1 (c) M13mp11/pUC13-polylinker
                 68-  87    20-   1 (c) T7 promoter
                 98-2158   620-2680     pUC13c
               2159-2345     1- 187     pUC13c
               2346-2624   654- 376 (c) pBR322
               2625-2869  xxxx-xxxx     phage SP6
               2851-2869     1-  19     SP6 promoter
               Conflict (cfl) and Mutations (mut): none
            FEATURE
                       1           start of SP6-RNA synthesis
                      69       (c) start of T7-RNA synthesis
               1101-1889 789-1 (c) Ap-R; b-lactamase
            POLYLINKER  HindIII-PstI-SalI-XbaI-BamHI-SmaI-SacI-EcoRI SELECTION
            #resistance  Ap
            SUMMARY  pGEM2    #length 2869   #checksum 1597.
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="synthetic construct"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:32630"
BASE COUNT          711 a          742 c          727 g          689 t
ORIGIN      
        1 gaatacacgg aattcgagct cgcccgggga tcctctagag tcgacctgca gcccaagctt
       61 ccggtctccc tatagtgagt cgtattaatt tcgataagcc agctgcatta atgaatcggc
      121 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
      781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
      841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
      961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1141 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1501 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1741 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     1801 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     1861 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     1921 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     1981 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2041 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     2101 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     2161 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     2221 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     2281 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     2341 catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     2401 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     2461 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     2521 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     2581 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta gcgatgaccc
     2641 tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga aactcagaag
     2701 gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc
     2761 agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat
     2821 taatacataa ccttatgtat catacacata cgatttaggt gacactata
//