LOCUS SYNPBR328V 4907 bp DNA circular SYN 26-JUL-1993
DEFINITION pBR328 cloning vector.
ACCESSION L08858
VERSION L08858.1
KEYWORDS .
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 4907)
AUTHORS Gilbert,W.
TITLE Obtained from VecBase 3.0
JOURNAL Unpublished
COMMENT These data and their annotation were supplied to GenBank by Will
Gilbert under the auspices of the GenBank Currator Program. pBR328
- Cloning Vector
ENTRY PBR328 #TYPE DNA CIRCULAR TITLE pBR328 -
Cloning Vector
DATE 12-SEP-1986
#sequence 16-DEC-1986
ACCESSION VB0004
SOURCE artificial
REFERENCE
#number 1
#authors Soberon X., Covarrubias L., Bolivar F.
#journal Gene (1982) 9: 287-305
#comment pBR328, residues 1-1431, 2521-4356
REFERENCE
#number 2
#authors Pouwels P.H., Enger-Valk B.E., Brammar W.J.
#book Cloning Vectors, Elsvier 1985 and supplements
#comment vector I-A-iv-4
COMMENT
Assembled from pBR325, Fragment 'TTCT',5-1431,2521-5996
by F. Pfeiffer, MPI, Martinsried
Revised 16-DEC-1986 by F. Pfeiffer:
1642/3 'AT' to 'TA' to match revised sequence of PBR322 COMMENT
pBR328 is a deletion derivative of pBR325.
KEYWORDS
CROSSREFERENCE
#parent
VecBase(3):pBR322, VecBase(3):pBR325
#offspring
VecBase(3):pBR329
PARENT
Features of pBR328 (4907 bp)
residue source
1-1431 1-1431 pBR322
1432-4907 2521-5996 pBR325
4431-4907 1311- 835 (c) pBR322
Conflict (cfl) and Mutations (mut): none
FEATURE
86-1276 1-1191 Tc-R; tetracycline resistance protein
2209-2997 789- 1 (c) Ap-R; b-lactamase
3477-4136 1- 660 Cm-R; Chloramphenicol-acetyltransferase
POLYLINKER none
SELECTION #resistance Ap, Tc, Cm
SUMMARY pBR328 #length 4907 #checksum 8335.
FEATURES Location/Qualifiers
source 1..4907
/organism="synthetic construct"
/mol_type="genomic DNA"
/db_xref="taxon:32630"
BASE COUNT 1131 a 1302 c 1264 g 1210 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg ccgcgttgct
1441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
1501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
1561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
1621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
1681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
1741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
1801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
1861 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
1921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
1981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
2041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
2101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
2161 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
2221 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
2281 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
2341 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
2401 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
2461 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
2521 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
2581 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
2641 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
2701 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
2761 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
2821 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
2881 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
2941 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
3001 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
3061 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
3121 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
3181 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
3241 taggcgtatc acgaggccct ttcgtcttcg aataaatacc tgtgacggaa gatcacttcg
3301 cagaataaat aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc
3361 gaaaatgaga cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
3421 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg
3481 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
3541 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
3601 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
3661 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
3721 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
3781 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
3841 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
3901 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
3961 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
4021 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
4081 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt
4141 tttaaggcag ttattggtgc ccttaaacgc ctggtgctac gcctgaataa gtgataataa
4201 gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt
4261 cgttaaatag ccgcttatgt ctattgctgg tttaccggtt tattgactac cggaagcagt
4321 gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta
4381 ataattgacg atatgatcat ttattctgcc tcccagagcc tgataaaaac ggtgaatccg
4441 ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac
4501 gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc
4561 atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta
4621 ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc
4681 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
4741 tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg
4801 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
4861 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgac
//