LOCUS SYNPBR325V 5996 bp DNA circular SYN 26-JUL-1993 DEFINITION pBR325 cloning vector. ACCESSION L08855 VERSION L08855.1 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct other sequences; artificial sequences. REFERENCE 1 (bases 1 to 5996) AUTHORS Gilbert,W. TITLE Obtained from VecBase 3.0 JOURNAL Unpublished COMMENT These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. pBR325 - Cloning Vector ENTRY PBR325 #TYPE DNA CIRCULAR TITLE pBR325 - Cloning Vector DATE 12-SEP-1986 #sequence 16-DEC-1986 ACCESSION VB0002 SOURCE artificial REFERENCE #number 1 #authors Prentki P., Karch F., Iida S., Meyer J. #journal Gene (1981) 14: 289-299 #comment pBR325, residues 4314-4419 and 5402-34 REFERENCE #number 2 #authors Alton N.K., Vapnek D. #journal Nature (1979) 282: 864-869 #comment Transposon Tn9, residues 4358-5444 This sequence differs from that shown in having 5150-C. REFERENCE #number 3 #authors Pouwels P.H., Enger-Valk B.E., Brammar W.J. #book Cloning Vectors, Elsvier 1985 and supplements #comment vector I-A-iv-4 COMMENT Revised 16-DEC-1986 by F. Pfeiffer: 2731/2 'AT' to 'TA' to match revised sequence of PBR322 COMMENT There is a sequence conflict in that pMAC58 contains a TTG sequence where its parent pBR325 at pos 5264-5265 only contains a TG. It is unclear, if this is a mutation in pMAC58, or if the sequence of pBR325 is incorrect at that position. COMMENT The plasmid pBR325 is a cloning vector constructed in vitro by the addition of the chloramphenicol resistance gene of an IS1-flanked transposon to pBR322. Sequence analysis suggests that this transposon is related to transposons Tn9 and Tn981 but is longer. No portion of insertion sequence IS1 was found in the genome. COMMENT This sequence was reconstructed from the genome of pBR322, transposons Tn9 and Tn981, and pBR325 genome fragments. The actual pBR325 and pBR328 genomes may differ from this sequence. COMMENT In pMAC58, a pBR325-derived vector, a TTG sequence was found where pBR325 has only a TG (pos 5264-65). It is not known if this discrepancy is a mutation or a sequence error in pBR325. COMMENT 1-5996/Genome of pBR325 4358-5444/Transposon Tn9 4358-5263/Transposon Tn981 86-1273/Protein: Tetracycline resistance protein 1917-2105/Protein: Regulatory protein rop 2271(C)/cAMP-dependent pBR-P4 transcription initiation site 4155-3298(C)/Protein: Penicillinase (EC 3.5.2.6) precursor 4155-4087(C)/Signal sequence 4086-3298(C)/Penicillinase 4566-5222/Protein: Chloramphenicol acetyltransferase (EC 2.3.1.28) KEYWORDS CROSSREFERENCE #prerevised PIRN(30):pBR325 #parent VecBase(3):pBR322, GenBank(50):Trn9Cat #offspring VecBase(3):pBR328, VecBase(3):pBR329, VecBase(3):pJSC73, VecBase(3):pMAC58, VecBase(3):pMAC5bg14 VecBase(3):pSV2Cat, VecBase(3):YRp17, VecBase(3):pAA37X PARENT Features of pBR325 (5996 bp) residue source 1- 5 834- 830 (c) pBR322 5-4358 5-4358 pBR322 4358-5444 36-1122 Tn9 5520-5996 1311- 835 (c) pBR322 Conflict (cfl) and Mutations (mut): pBR325 source mut 5150 T C 828 Tn9 FEATURE residue source 86-1276 1-1191 Tc-R; tetracycline resistance protein 3298-4086 789- 1 (c) Ap-R; b-lactamase 4566-5225 1- 660 Cm-R; chloramphenicol acetyltransferase POLYLINKER none SELECTION #resistance Ap, Tc, Cm SUMMARY pBR325 #length 5996 #checksum 4248. FEATURES Location/Qualifiers source 1..5996 /organism="synthetic construct" /mol_type="genomic DNA" /db_xref="taxon:32630" BASE COUNT 1395 a 1600 c 1565 g 1436 t ORIGIN 1 aggccatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg 541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct 601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat 661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa 901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt 961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc 1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca 1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc 1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat 1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg 1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga 1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac 1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg 1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg 1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc 1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg 1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca 1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga 1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa 1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt 1861 ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg catcaagtga 1921 ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc 1981 ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc 2041 acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa 2101 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 2161 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga 2221 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat 2281 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 2341 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 2401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 2461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 2521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 2581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 2641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 2701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 2761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 2821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 2881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 2941 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 3001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 3061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 3121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 3181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 3241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 3301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 3361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 3421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 3481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 3541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 3601 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 3661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 3721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 3781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 3841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 3901 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 3961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 4021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 4081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 4141 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 4201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 4261 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 4321 ctataaaaat aggcgtatca cgaggccctt tcgtcttcga ataaatacct gtgacggaag 4381 atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca 4441 acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg 4501 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag 4561 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta 4621 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc 4681 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct 4741 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag 4801 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa 4861 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca 4921 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta 4981 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa 5041 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc 5101 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct 5161 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg 5221 cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag 5281 tgataataag cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca 5341 gggcagggtc gttaaatagc cgcttatgtc tattgctggt ttaccggttt attgactacc 5401 ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggcc agtttgctca ggctctcccc 5461 gtggaggtaa taattgacga tatgatcatt tattctgcct cccagagcct gataaaaacg 5521 gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg 5581 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca 5641 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga 5701 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat 5761 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa 5821 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca 5881 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 5941 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgac //