LOCUS SYNPAT153V 3658 bp DNA circular SYN 26-JUL-1993
DEFINITION pAT153 cloning vector.
ACCESSION L08853
VERSION L08853.1
KEYWORDS .
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 3658)
AUTHORS Gilbert,W.
TITLE Obtained from VecBase 3.0
JOURNAL Unpublished
COMMENT These data and their annotation were supplied to GenBank by Will
Gilbert under the auspices of the GenBank Currator Program. pAT153
- Cloning vector
ENTRY PAT153 #TYPE DNA CIRCULAR TITLE pAT153 -
Cloning vector
DATE 07-MAY-1986
#sequence 16-DEC-1986
ACCESSION VB0007
SOURCE artificial
REFERENCE
#number 1
#authors Twigg A.J., Sherratt D.
#journal Nature (1980) 283: 216-218
REFERENCE
#number 2
#authors Pouwels P.H., Enger-Valk B.E., Brammar W.J.
#book Cloning Vectors, Elsvier 1985 and supplements
#comment vector I-A-iv-3
COMMENT
Assembled by F. Pfeiffer, MPI, Martinsried
Revised 16-DEC-1986 by F. Pfeiffer:
2026/7 'AT' to 'TA' to match revised sequence of PBR322 COMMENT
pAT153 was constructed by removal of HaeII-fragments B
and H (pos. 1649-2353) from pBR322.
COMMENT
There is confliciting information on the structure of pAT153.
some 200
bases may be erraneuosly missing from this sequence. KEYWORDS
CROSSREFERENCE
#parent
VecBase(3):pBR322
#offspring
VecBase(3):pWT511, VecBase(3):pWT551, VecBase(3):pWT571 PARENT
Features of pAT153 (3658 bp)
residue source
1-1648 1-1648 pBR322
1649-3658 2354-4363 pBR322
Conflict (cfl) and Mutations (mut): none
FEATURE
86-1276 1-1191 Tc-R; tetracycline resistance protein
2593-3381 789- 1 (c) Ap-R; b-lactamase
POLYLINKER none
SELECTION #resistance Tc, Ap
SUMMARY pAT153 #length 3658 #checksum 7917.
FEATURES Location/Qualifiers
source 1..3658
/organism="synthetic construct"
/mol_type="genomic DNA"
/db_xref="taxon:32630"
BASE COUNT 812 a 1011 c 952 g 883 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
1621 taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg
1681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
1741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
1801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
1861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
1921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
1981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
2041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
2101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
2161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
2221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
2281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
2341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
2401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
2461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
2521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
2581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
2641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
2701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
2761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
2821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
2881 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
2941 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
3001 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
3061 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
3121 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
3181 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
3241 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
3301 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
3361 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
3421 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
3481 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
3541 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
3601 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa
//