LOCUS KY930498 8353 bp DNA circular SYN 01-APR-2018
DEFINITION Chloroplast expression vector pCEV2, complete sequence.
ACCESSION KY930498
VERSION KY930498.1
KEYWORDS .
SOURCE Chloroplast expression vector pCEV2
ORGANISM Chloroplast expression vector pCEV2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8353)
AUTHORS Chen,P.-Y. and To,K.-Y.
TITLE Construction of chloroplast expression vectors for higher plants
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8353)
AUTHORS Chen,P.-Y. and To,K.-Y.
TITLE Direct Submission
JOURNAL Submitted (11-APR-2017) Agricultural Biotechnology Research Center,
Academia Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei
11529, Taiwan
FEATURES Location/Qualifiers
source 1..8353
/organism="Chloroplast expression vector pCEV2"
/mol_type="other DNA"
/db_xref="taxon:2127038"
rep_origin complement(428..1016)
/note="pUC19 replication origin"
CDS complement(1187..2047)
/note="confers ampicillin resistance"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AVQ67765.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 2655..4648
/note="tomato left targeting region; contains 16S rRNA and
trnI"
rep_origin 4072..4153
/note="chloroplast replication origin; oriA"
regulatory 4655..4902
/regulatory_class="promoter"
/note="atpI promoter; PatpI"
misc_feature 4903..4918
/note="cloning site"
regulatory 4919..5087
/regulatory_class="terminator"
/note="rps16 terminator; Trps16"
regulatory 5094..5312
/regulatory_class="promoter"
/note="rrn promoter; Prrn"
gene 5319..6110
/gene="aadA"
CDS 5319..6110
/gene="aadA"
/note="confers spectinomycin resistance"
/codon_start=1
/transl_table=11
/product="aminoglycoside 3'-adenyltransferase"
/protein_id="AVQ67766.1"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
regulatory 6117..6338
/regulatory_class="terminator"
/note="psbA terminator; TpsbA"
misc_feature 6345..8348
/note="tomato right targeting region; contains trnA and
23S rRNA"
BASE COUNT 2125 a 1929 c 2264 g 2035 t
ORIGIN
1 ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
61 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
121 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
181 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
241 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
301 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
361 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
421 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
481 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
541 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
601 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
661 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
721 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
781 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
841 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
901 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
961 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
1021 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
1081 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
1141 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
1201 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
1261 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
1321 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
1381 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
1441 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
1501 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
1561 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
1621 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
1681 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
1741 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
1801 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
1861 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
1921 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
1981 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
2041 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
2101 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
2161 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
2221 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
2281 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
2341 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
2401 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
2461 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
2521 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
2581 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
2641 gtgaattcga gctctaccaa gctggagtac ggtaggggca gagggaattt ccggtggagc
2701 ggtgaaatgc gtagagatcg gaaagaacac caacggcgaa agcactctgc tgggccgaca
2761 ctgacactga gagacgaaag ctaggggagc gaatgggatt agatacccca gtagtcctag
2821 ccgtaaacga tggatactag gcgctgtgcg tatcgacccg tgcagtgctg tagctaacgc
2881 gttaagtatc ccgcctgggg agtacgttcg caagaatgaa actcaaagga attgacgggg
2941 gcccgcacaa gcggtggagc atgtggttta attcgatgca aagcgaagaa ccttaccagg
3001 gcttgacatg ccgcgaatcc tcttgaaaga gaggggtgcc ttcgggaacg cggacacagg
3061 tggtgcatgg ctgtcgtcag ctcgtgccgt aaggtgttgg gttaagtccc gcaacgagcg
3121 caaccctcgt gtttagttgc catcgttgag tttggaaccc tgaacagact gccggtgata
3181 agccggagga aggtgaggat gacgtcaagt catcatgccc cttatgccct gggcgacaca
3241 cgtgctacaa tggccgggac aaagggtcgc gatcccgcga gggtgagcta accccaaaaa
3301 cccgtcctca gttcggattg caggctgcaa ctcgcctgca tgaagccgga atcgctagta
3361 atcgccggtc agccatacgg cggtgaattc gttcccgggc cttgtacaca ccgcccgtca
3421 cactatggga gctggccatg cccgaagtcg ttaccttaac cgcaaggagg gggatgccga
3481 aggcagggct agtgactgga gtgaagtcgt aacaaggtag ccgtactgga aggtgcggct
3541 ggatcacctc cttttcaggg agagctaatg cttgttgggt attttggttt gacactgctt
3601 cacaccccca aaaaaaagaa gggagctacg tctgagttaa acttggagat ggaagtcttc
3661 tttcctttct cgacggtgaa gtaagaccaa gctcatgagc ttattatcct aggtcggaac
3721 aagttgatag gacccccttt tttacgtccc tatgttcccc ccgtgtggcg acatgggggc
3781 gaaaaaagga aagagaggga tggggtttct ctcgcttttg gcatagcggg cccccagtgg
3841 gaggctcgca cgacgggcta ttagctcagt ggtagagcgc gcccctgata attgcgtcgt
3901 tgtgcctggg ctgtgagggc tctcagccac atggatagtt caatgtgctc atcggcgcct
3961 gaccctgaga tgtggatcat ccaaggcaca ttagcatggc gtactcctcc tgttcgaacc
4021 ggggtttgaa accaaactcc tcctcaggag gatagatggg gcgattcggg tgagatccaa
4081 tgtagatcca actttcgatt cactcgtggg atccgggcgg tccggggggg accaccacgg
4141 ctcctctctt ctcgagaatc catacatccc ttatcagtgt atggacagct atctctcgag
4201 cacaggttta ggttcggcct caatgggaaa ataaaatgga gcacctaaca acgcatcttc
4261 acagaccaag aactacgaga tcaccccttt cattctgggg tgacggaggg atcgtaccat
4321 tcgagccgtt tttttcttga ctcgaaatcg aaatgggagc aggtttgaaa aaggatctta
4381 gagtgtctag ggttgggcca ggagggtctc ttaacgcctt cttttttctt ctcatcggag
4441 ttatttcaca aagacttgcc agggtaagga agaagggggg aacaagcaca cttggagagc
4501 gcagtacaac ggagagttgt atgctgcgtt cgggaaggat gaatcgctcc cgaaaaggaa
4561 tctattgatt ctctcccaat tggttggacc gtaggtgcga tgatttactt cacgggcgag
4621 gtctctggtt caagtccagg atggcccaga gctctctagc tatataagaa atccttgatt
4681 aataataaca taataagata aataacttac ttcagaaatc ccttatggaa tcgcttacta
4741 tttctgaatt tcaaaaaaga gataaaaata gctggggata ttatgtgatt tattagtatt
4801 ctaaatctta gttggtattc aaaatatccg attcaagtag acaaagagat ggttgaatca
4861 aaaaattttg tttaaagttc aattttttca gagggcaagg cagcggccgc ggcgcgcctc
4921 aaccgaaatt caattaagga aataaattaa ggaaatacaa aaaggggggt agtcatttgt
4981 atataacttt gtatgacttt tctcttctat ttttttgtat ttcctccctt tccttttcta
5041 tttgtatttt tttatcattg cttccattga attccgtgtt ctataacggt accatcagtt
5101 cgagcctgat tatccctaag cccaatgtga gtttttctag ttggatttgc tcccccgccg
5161 tcgttcaatg agaatggata agaggctcgt gggattgacg tgagggggca gggatggcta
5221 tatttctggg agcgaactcc gggcgaatat gaagcgcatg gatacaagtt atgccttgga
5281 atgaaagaca attccgaatc cgctttgtct acggatccat gagggaagcg gtgatcgccg
5341 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
5401 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
5461 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
5521 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
5581 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
5641 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
5701 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
5761 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
5821 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
5881 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
5941 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
6001 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
6061 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tctagagatc
6121 ctggcctagt ctataggagg ttttgaaaag aaaggagcaa taatcatttt cttgttctat
6181 caagagggtg ctattgctcc tttctttttt tctttttatt tatttactag tattttactt
6241 acatagactt ttttgtttac attatagaaa aagaaggaga ggttattttc ttgcatttat
6301 tcatgattga gtattctatt ttgattttgt atttgtttct gcagctgcgc cagggaaaag
6361 aatagaagaa gcatctgact acttcatgca tgctccactt ggctcggggg gatatagctc
6421 agttggtaga gctccgctct tgcaattggg tcgttgcgat tacgggttgg atgtctaatt
6481 gtccaggcgg taatgatagt atcttgtacc tgaaccggtg gctcactttt tctaagtaat
6541 ggggaagagg accgaaacgt gccactgaaa gactctactg agacaaagat gggctgtcaa
6601 gaacgtagag gaggtaggat gggcagttgg tcagatctag tatggatcgt acatggacgg
6661 tagttggagt cggcggctct cccagggttc cctcatctga gatctctggg gaagaggatc
6721 aagttggccc ttgcgaacag cttgatgcac tatctccctt caaccctttg agcgaaatgc
6781 ggcaaaagaa aaggaaggaa aatccatgga ccgaccccat catctccacc ccgtaggaac
6841 tacgagatca ccccaaggac gccttcggca tccaggggtc acggaccgac catagaaccc
6901 tgttcaataa gtggaacgca ttagctgtcc gctctcaggt tgggcagtca gggtcggaga
6961 agggcaatga ctcattctta aaaccagcgt tcttaagacc aaagagtcgg gcggaagggg
7021 gggaaagccc tccgttcctg gttctcctgt agttggatcc tccggaacca caagaatcct
7081 tagttagaat gggattccaa ctcagcacct tttgagtgag attttgagaa gagttgctct
7141 ttggagagca cagtacgatg aaagttgtaa gctgtgttcg ggggggagtt attgtctatc
7201 gttggcctct atggtagaat cagtcggggg acctgagagg cggtggttta ccctgcggcg
7261 gatgtcagcg gttcgagtcc gcttatctcc aactcgtgaa cttagccgat acaaagcttt
7321 atgatagcac ccaatttttc cgattcggcg gttcgatcta tgatttatca ttcatggacg
7381 ttgataagat ccatccattt agcagcacct taggatggca tagccttaaa agtgaagggc
7441 gaggttcaaa cgaggaaagg cttacggtgg atacctaggc acccagagac gaggaagggc
7501 gtagtaatcg acgaaatgct tcggggagtt gaaaataagc atagatccgg agattcccga
7561 atagggcaac ctttcgaact gctgctgaat ccatgggcag gcaagagaca acctggcgaa
7621 ctgaaacatc ttagtagcca gaggaaaaga aagcaaaagc gattcccgta gtagcggcga
7681 gcgaaatggg agcagcctaa accgtgaaaa cggggttgtg ggagagcaat acaagcgtcg
7741 tgctgctagg cgaagcagcc tgaatgctgc accctagatg gcgaaagtcc agtagccgaa
7801 agcatcacta gcttacgctc tgacccgagt agcatggggc acgtggaatc ccgtgtgaat
7861 cagcaaggac caccttgcaa ggctaaatac tcctgggtga ccgatagcga agtagtaccg
7921 tgagggaagg gtgaaaagaa cccccatcgg ggagtgaaat agaacatgaa accgtaagct
7981 cccaagcagt gggaggagcc agggctctga ccgcgtgcct gttgaagaat gagccggcga
8041 ctcataggca gtggcttggt taagggaacc caccggagcc gtagcgaaag cgagtcttca
8101 tagggcaatt gtcactgctt atggacccga acctgggtga tctatccatg accaggatga
8161 agcttgggtg aaactaagtg gaggtccgaa ccgactgatg ttgaagaatc agcggatgag
8221 ttgtggttag gggtgaaatg ccactcgaac ccagagctag ctggttctcc ccgaaatgcg
8281 ttgaggcgca gcagttgact ggacatctag gggtaaagca ctgtttcggt gcgggccgcg
8341 agagcggtct gca
//